Analysis of wild-species introgressions in tomato inbreds uncovers ancestral origins

Menda, Naama; Strickler, Susan R.; Edwards, Jeremy D.; Bombarely, Aureliano; Dunham, Diane; Martin, Gregory B.; Mejía, Luis C.; Hutton, Samuel F.; Havey, Michael J.; Maxwell, D. P.; Mueller, Lukas A.

doi:10.1186/s12870-014-0287-2

Cited by 31 publications

(18 citation statements)

References 51 publications

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“…Breeders have long recognized the value of crop wild relatives (CWRs) as sources of novel beneficial variation for resistance or tolerance to stresses (Bessey, 1906;Harlan and de Wet, 1971;Hajjar and Hodgkin, 2007). In this respect, most of the modern varieties of several important crops, like wheat or tomato, carry introgressions from wild relatives (Menda et al, 2014;Wulff and Moscou, 2014). CWRs are wild species that are able to exchange genes with the cultivated taxa through sexual or somatic hybridization (Harlan andde Wet, 1971, Maxted et al, 2006).…”

Section: Introductionmentioning

confidence: 99%

Introgressiomics: a new approach for using crop wild relatives in breeding for adaptation to climate change

et al. 2017

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The need to boost agricultural production in the coming decades in a climate change scenario requires new approaches for the development of new crop varieties that are more resilient and more efficient in the use of resources. Crop wild relatives (CWRs) are a source of variation for many traits of interest in breeding, in particular tolerance to abiotic and biotic stresses. However, their potential in plant breeding has largely remained unexploited. CWRs can make an effective contribution to broadening the genetic base of crops and to introgressing traits of interest, but their direct use by breeders in breeding programs is usually not feasible due to the presence of undesirable traits in CWRs (linkage drag) and frequent breeding barriers with the crop. Here we call for a new approach, which we tentatively call 'introgressiomics', which consists of mass scale development of plant materials and populations with introgressions from CWRs into the genetic background of crops. Introgressiomics is a form of

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Section: Introductionmentioning

confidence: 99%

Introgressiomics: a new approach for using crop wild relatives in breeding for adaptation to climate change

et al. 2017

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“…In the case of single, historic, and widely used alleles such as Pm-0 , the potential for historical recombination events in at least some cultivars to have reduced the size of the candidate interval around the gene of interest is high, barring chromosomal inversions or other rearrangements present in the region containing the introgression. Previously, this approach has been used to map other major resistance genes derived from wild species in tomato [37, 38]. Our study has advantages over previous efforts in that only one gene from one wild donor species is known to be widespread among current cultivars for the trait of interest.…”

Section: Introductionmentioning

confidence: 99%

Cultivar-Based Introgression Mapping Reveals Wild Species-Derived Pm-0, the Major Powdery Mildew Resistance Locus in Squash

Holdsworth

LaPlant

Bell³

et al. 2016

PLoS ONE

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Powdery mildew is a major fungal disease on squash and pumpkin (Cucurbita spp.) in the US and throughout the world. Genetic resistance to the disease is not known to occur naturally within Cucurbita pepo and only infrequently in Cucurbita moschata, but has been achieved in both species through the introgression of a major resistance gene from the wild species Cucurbita okeechobeensis subsp. martinezii. At present, this gene, Pm-0, is used extensively in breeding, and is found in nearly all powdery mildew-resistant C. pepo and C. moschata commercial cultivars. In this study, we mapped C. okeechobeensis subsp. martinezii-derived single nucleotide polymorphism (SNP) alleles in a set of taxonomically and morphologically diverse and resistant C. pepo and C. moschata cultivars bred at Cornell University that, by common possession of Pm-0, form a shared-trait introgression panel. High marker density was achieved using genotyping-by-sequencing, which yielded over 50,000 de novo SNP markers in each of the three Cucurbita species genotyped. A single 516.4 kb wild-derived introgression was present in all of the resistant cultivars and absent in a diverse set of heirlooms that predated the Pm-0 introgression. The contribution of this interval to powdery mildew resistance was confirmed by association mapping in a C. pepo cultivar panel that included the Cornell lines, heirlooms, and 68 additional C. pepo cultivars and with an independent F2 population derived from C. okeechobeensis subsp. martinezii x C. moschata. The interval was refined to a final candidate interval of 76.4 kb and CAPS markers were developed inside this interval to facilitate marker-assisted selection.

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“…However, disease resistance traits have been disregarded, resulting in cultivars highly susceptible to many pathogens (Foolad, 2007). Wild tomato species, in contrast, such as S. chilense, S. peruvianum, S. habrochaites, S. parviflorum, S. pennellii or S. pimpinellifolium, are considered a rich source of disease resistance genes and have been utilized in breeding programmes for the introgression of these genes into cultivated varieties to improve their resistance (Menda et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

Diverse responses of wild and cultivated tomato to BABA, oligandrin andOidium neolycopersiciinfection

Satková

Starý

Plešková

et al. 2016

Ann Bot

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Background and Aims Current strategies for increased crop protection of susceptible tomato plants against pathogen infections include treatment with synthetic chemicals, application of natural pathogen-derived compounds or transfer of resistance genes from wild tomato species within breeding programmes. In this study, a series of 45 genes potentially involved in defence mechanisms was retrieved from the genome sequence of inbred reference tomato cultivar Solanum lycopersicum 'Heinz 1706'. The aim of the study was to analyse expression of these selected genes in wild and cultivated tomato plants contrasting in resistance to the biotrophic pathogen Oidium neolycopersici, the causative agent of powdery mildew. Plants were treated either solely with potential resistance inducers or by inducers together with the pathogen.Methods The resistance against O. neolycopersici infection as well as RT-PCR-based analysis of gene expression in response to the oomycete elicitor oligandrin and chemical agent b-aminobutyric acid (BABA) were investigated in the highly susceptible domesticated inbred genotype Solanum lycopersicum 'Amateur' and resistant wild genotype Solanum habrochaites.Key Results Differences in basal expression levels of defensins, germins, b-1,3-glucanases, heveins, chitinases, osmotins and PR1 proteins in non-infected and non-elicited plants were observed between the highly resistant and susceptible genotypes. Moreover, these defence genes showed an extensive up-regulation following O. neolycopersici infection in both genotypes. Application of BABA and elicitin induced expression of multiple defence-related transcripts and, through different mechanisms, enhanced resistance against powdery mildew in the susceptible tomato genotype.Conclusions The results indicate that non-specific resistance in the resistant genotype S. habrochaites resulted from high basal levels of transcripts with proven roles in defence processes. In the susceptible genotype S. lycopersicum 'Amateur', oligandrin-and BABA-induced resistance involved different signalling pathways, with BABAtreated leaves displaying direct activation of the ethylene-dependent signalling pathway, in contrast to previously reported jasmonic acid-mediated signalling for elicitins.

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Analysis of wild-species introgressions in tomato inbreds uncovers ancestral origins

Cited by 31 publications

References 51 publications

Introgressiomics: a new approach for using crop wild relatives in breeding for adaptation to climate change

Introgressiomics: a new approach for using crop wild relatives in breeding for adaptation to climate change

Cultivar-Based Introgression Mapping Reveals Wild Species-Derived Pm-0, the Major Powdery Mildew Resistance Locus in Squash

Diverse responses of wild and cultivated tomato to BABA, oligandrin andOidium neolycopersiciinfection

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