Ty-3, a begomovirus resistance locus near the Tomato yellow leaf curl virus resistance locus Ty-1 on chromosome 6 of tomato

Ji, Yuanfu; Schuster, David J.; Scott, John F.

doi:10.1007/s11032-007-9089-7

Cited by 150 publications

(55 citation statements)

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“…The Ty - 3 locus has generally shown less dominance, but a wider range of resistance against TYLCV strains and bipartite begomoviruses (Ji et al 2007a). Hybrids with the heterozygous combination of both genes may prove to be effective and durable against a wide array of begomoviruses.…”

Section: Discussionmentioning

confidence: 99%

“…These viruses cause serious losses to tomato ( Solanum lycopersicum L.) production in many tropical and subtropical regions in the world (Ji et al 2007a; Cohen and Lapidot 2007). Whitefly control measures such as the use of insecticides and/or fine-mesh screens or UV-absorbing plastic films/screens can limit disease damage, but epidemics can still occur.…”

Section: Introductionmentioning

confidence: 99%

“…Species that have demonstrated resistance include S. pimpinellifolium, S. peruvianum, S. chilense, S. habrochaites , and S. cheesmaniae (Ji et al 2007b; Pico et al 1996; Scott 2007; Vidavski 2007). So far, as many as five resistance loci have been mapped, i.e., the dominant genes including Ty - 1 , Ty - 2 , Ty - 3 , Ty - 4 , and recessive gene ty - 5 (Zamir et al 1994; Hanson et al 2000; Ji et al 2007a, 2009b; Anbinder et al 2009). Ty - 1 and Ty - 3 were each derived from S. chilense and mapped to nearby positions on chromosome 6 (Ji et al 2007c); however, Verlaan et al (2013) demonstrated that Ty - 1 and Ty - 3 are alleles of the same gene.…”

Section: Introductionmentioning

confidence: 99%

“…Yet, neither gene is effective against bipartite begomoviruses, and the resistance of both has been overcome by some strains of TYLCV (Ji et al 2007a). There is evidence, however, that Ty - 2 can provide an enhanced level of resistance to bipartite begomoviruses when pyramided with Ty - 3 (Mejía et al 2005), potentially making it a more attractive tool to breeders.…”

Section: Introductionmentioning

confidence: 99%

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Fine mapping of the tomato yellow leaf curl virus resistance gene Ty-2 on chromosome 11 of tomato

et al. 2014

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Resistances to begomoviruses, including bipartite tomato mottle virus and monopartite tomato yellow leaf curl virus (TYLCV), have been introgressed to cultivated tomato (Solanum lycopersicum) from wild tomato accessions. A major gene, Ty-2 from S. habrochaites f. glabratum accession “B6013,” that confers resistance to TYLCV was previously mapped to a 19-cM region on the long arm of chromosome 11. In the present study, approximately 11,000 plants were screened and nearly 157 recombination events were identified between the flanking markers C2_At1g07960 (82.5 cM, physical distance 51.387 Mb) and T0302 (89 cM, 51.878 Mb). Molecular marker analysis of recombinants and TYLCV evaluation of progeny from these recombinants localized Ty-2 to an approximately 300,000-bp interval between markers UP8 (51.344 Mb) and M1 (51.645 Mb). No recombinants were identified between TG36 and C2_At3g52090, a region of at least 115 kb, indicating severe recombination suppression in this region. Due to the small interval, fluorescence in situ hybridization analysis failed to clarify whether recombination suppression is caused by chromosomal rearrangements. Candidate genes predicted based on tomato genome annotation were analyzed by RT-PCR and virus-induced gene silencing. Results indicate that the NBS gene family present in the Ty-2 region is likely not responsible for the Ty-2-conferred resistance and that two candidate genes might play a role in the Ty-2-conferred resistance. Several markers very tightly linked to the Ty-2 locus are presented and useful for marker-assisted selection in breeding programs to introgress Ty-2 for begomovirus resistance.Electronic supplementary materialThe online version of this article (doi:10.1007/s11032-014-0072-9) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Fine mapping of the tomato yellow leaf curl virus resistance gene Ty-2 on chromosome 11 of tomato

et al. 2014

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“…Molecular scanning by sequencing PCR fragments showed that Gh13 had an introgression on chromosome 6 from 20 to 32 cM (C. Martin and D.P. Maxwell, personal communication), which corresponds to the location of the Ty-3 locus [47,48]. Gh13 was used in several research projects to determine the effectiveness of the Ty-3 locus in conferring resistance to begomoviruses [40,49].…”

Section: Methodsmentioning

confidence: 99%

Analysis of wild-species introgressions in tomato inbreds uncovers ancestral origins

et al. 2014

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BackgroundDecades of intensive tomato breeding using wild-species germplasm have resulted in the genomes of domesticated germplasm (Solanum lycopersicum) being intertwined with introgressions from their wild relatives. Comparative analysis of genomes among cultivated tomatoes and wild species that have contributed genetic variation can help identify desirable genes, such as those conferring disease resistance. The ability to identify introgression position, borders, and contents can reveal ancestral origins and facilitate harnessing of wild variation in crop breeding.ResultsHere we present the whole-genome sequences of two tomato inbreds, Gh13 and BTI-87, both carrying the begomovirus resistance locus Ty-3 introgressed from wild tomato species. Introgressions of different sizes on chromosome 6 of Gh13 and BTI-87, both corresponding to the Ty-3 region, were identified as from a source close to the wild species S. chilense. Other introgressions were identified throughout the genomes of the inbreds and showed major differences in the breeding pedigrees of the two lines. Interestingly, additional large introgressions from the close tomato relative S. pimpinellifolium were identified in both lines. Some of the polymorphic regions were attributed to introgressions in the reference Heinz 1706 genome, indicating wild genome sequences in the reference tomato genome.ConclusionsThe methods developed in this work can be used to delineate genome introgressions, and subsequently contribute to development of molecular markers to aid phenotypic selection, fine mapping and discovery of candidate genes for important phenotypes, and for identification of novel variation for tomato improvement. These universal methods can easily be applied to other crop plants.Electronic supplementary materialThe online version of this article (doi:10.1186/s12870-014-0287-2) contains supplementary material, which is available to authorized users.

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Integrated Approaches to Manage Tomato Yellow Leaf Curl Viruses

Kadirvel

Venkatesan

Kenyon

et al. 2010

Biocatalysis and Biomolecular Engineering

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Ty-3, a begomovirus resistance locus near the Tomato yellow leaf curl virus resistance locus Ty-1 on chromosome 6 of tomato

Abstract: The column headings of the markers in Table 5 of the original were incorrect. The correct headings are TG231 (left column) and PG3 (right column). The online version of the original article can be found at http:// dx.

Cited by 150 publications

References 34 publications

Fine mapping of the tomato yellow leaf curl virus resistance gene Ty-2 on chromosome 11 of tomato

Fine mapping of the tomato yellow leaf curl virus resistance gene Ty-2 on chromosome 11 of tomato

Analysis of wild-species introgressions in tomato inbreds uncovers ancestral origins

Integrated Approaches to Manage Tomato Yellow Leaf Curl Viruses

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