Molecular Mapping of Ty-4, a New Tomato Yellow Leaf Curl Virus Resistance Locus on Chromosome 3 of Tomato

Ji, Yuanfu; Scott, John F.; Schuster, David J.; Maxwell, D. P.

doi:10.21273/jashs.134.2.281

Cited by 136 publications

(99 citation statements)

References 23 publications

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“…However, results obtained here are compatible with the location for Ty-3, the resistance locus present in these lines (Ji et al 2007b). An additional locus, Ty-4, conferring lesser effect on TYLCV resistance in these same breeding lines derived from LA1932 has been described on the long arm of chromosome 3 (Ji et al 2009a). Lines developed in the present work do not show an S. chilense introgression for this region in chromosome 3.…”

Section: Discussionsupporting

confidence: 47%

“…However, recent work developed with better marker coverage, suggested that Ty-1 and Ty-3 are likely allelic (Verlaan et al 2011). Another TYLCV resistance locus, Ty-4, also derived from LA1932 but conferring a lesser effect on TYLCV resistance, has been mapped on chromosome 3 (Ji et al 2009a). Other resistance loci have been identified from different wild tomato relatives: Ty-2, on chromosome 11 and derived from S. habrochaites (Hanson et al 2006;Ji et al 2009b), and Ty-5, originated from S. peruvianum and mapped on chromosome 4 (Anbinder et al 2009).…”

Section: Introductionmentioning

confidence: 99%

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Genetic control and mapping of Solanum chilense LA1932, LA1960 and LA1971-derived resistance to Tomato yellow leaf curl disease

2012

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Tomato yellow leaf curl disease (TYLCD) is caused by a complex of begomovirus. Breeding for resistance to this disease has mainly been based on Ty-1 gene, derived from Solanum chilense LA1969. Commercial varieties available to date still develop symptoms and suffer yield losses with high inoculum pressure and early infections. It is of interest to incorporate in breeding programs resistance from the different available sources. Lines with resistance to TYLCD derived from S. chilense accessions LA1932, LA1960 and LA1971 were previously developed. The objectives of this work were to study the genetic control of the resistance derived from these accessions and to map the resistance loci. Response to viral infection was assayed in segregating generations derived from these sources. Results obtained were compatible with a monogenic control of resistance. A total of 94 markers were used to locate the S. chilense introgressions in each of the lines. Only the presence of a large introgression in chromosome 6 was common to all lines. Analysis of recombinants allowed localizing the resistance loci in an interval of approximately 25 cM, also common to all five families. This interval includes the region to which two previously S. chilense-derived TYLCD resistance loci have been mapped, the Ty-1/Ty-3 region. This is the first report of LA1960 and LA1971-derived TYLCV resistance loci to be located on chromosome 6. Further work will be done to fine map the loci found in the present work, in order to determine if they are indeed located in the Ty-1/Ty-3 region.

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Section: Discussionsupporting

confidence: 47%

Section: Introductionmentioning

confidence: 99%

Genetic control and mapping of Solanum chilense LA1932, LA1960 and LA1971-derived resistance to Tomato yellow leaf curl disease

2012

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“…Similarly, Ty-2 gene was recently fine mapped and candidate genes that might play role in resistance were identified (Yang et al 2014). An additional gene, Ty-4, was mapped in some lines derived from LA1932 on the chromosome 3 that accounted for only 16 % of the variation in symptom severity (Ji et al 2009b). The mapping results of TYLCD resistance in TY172 showed that the resistance is controlled by a major QTL (ty-5) on chromosome 4 that accounted for nearly 46 % of variation in symptom severity (Anbinder et al 2009).…”

Section: Introductionmentioning

confidence: 99%

Marker assisted selection of Ty-2 and Ty-3 carrying tomato lines and their implications in breeding tomato leaf curl disease resistant hybrids

et al. 2015

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Tomato leaf curl virus disease caused by whitefly-transmitted begomoviruses is a serious tomato production constraint in tropical and subtropical regions of the world. Use of genetic resistance for diseases caused by begomoviruses is an important component of sustainable disease management approaches. To date, six introgressions (Ty genes) derived from different tomato wild species that confer resistance to tomato leaf curl disease have been identified. Among these, dominant Ty-2 and partially dominant Ty-3 are important for the development of resistant hybrids because of their gene action. In the present study we report marker assisted selection of tomato lines carrying Ty-2 and Ty-3 genes and the effect of combining these in tomato hybrids using agroinoculation. The response of lines and hybrids with the Ty-2 gene indicated ineffectiveness of Ty-2 to infection by a bipartite tomato leaf curl virus. The Ty-3 gene showed dosage effect with partial resistance in heterozygous state. However, the level of resistance in hybrids with the combination of Ty-2 and Ty-3 genes was higher and similar to homozygous Ty-3 plants.The Ty-2 gene in either homozygous or heterozygous state enhanced the level of resistance of plants with heterozygous Ty-3. Thus, tomato hybrid breeding programs can be benefited by using tomato lines carrying Ty-2 and Ty-3 resistance genes. The combined results of evaluation of horticultural performance, field resistance and agroinoculation tests demonstrated that the set of tomato lines developed in this study could be useful resources for achieving higher level of resistance in tomato hybrids.

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“…Currently five different resistance loci (Ty-1 through Ty-5) have been identified. These loci originate from different wild tomato accessions and possess various modes of TYLCV resistance (Agrama and Scott 2006;Anbinder et al 2009;Chagué et al 1997;Ji et al 2009;Zamir et al 1994.…”

Section: Introductionmentioning

confidence: 99%

“…Upon whitefly-mediated inoculation of TYLCV, the R line remains symptomless and contains barely detectable amounts of virus, while the S line presents the typical disease symptoms of stunting, leaf yellowing and leaf curling, and contains large amounts of virus. None of the polymorphic DNA markers tagging resistance to TYLCV from S. chilense (Ji et al 2009;Zamir et al 1994) or to Tomato leaf curl virus (TLCV) from S. habrochaites (Hanson et al 2000) have been found to be linked with resistance of line 902 (unpublished results).…”

Section: Introductionmentioning

confidence: 99%

Silencing of a single gene in tomato plants resistant to Tomato yellow leaf curl virus renders them susceptible to the virus

et al. 2009

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A reverse-genetics approach was applied to identify genes involved in Tomato yellow leaf curl virus (TYLCV) resistance, taking advantage of two tomato inbred lines from the same breeding program-one susceptible (S), one resistant (R-that used Solanum habrochaites as the source of resistance. cDNA libraries from inoculated and non-inoculated R and S plants were compared, postulating that genes preferentially expressed in the R line may be part of the network sustaining resistance to TYLCV. Further, we assumed that silencing genes located at important nodes of the network would lead to collapse of resistance. Approximately 70 different cDNAs representing genes preferentially expressed in R plants were isolated and their genes identified by comparison with public databases. A Permease I-like protein gene encoding a transmembranal transporter was further studied: it was preferentially expressed in R plants and its expression was enhanced several-fold following TYLCV inoculation. Silencing of the Permease gene of R plants using Tobacco rattle virus-induced gene silencing led to loss of resistance, expressed as development of disease symptoms typical of infected susceptible plants and accumulation of large amounts of virus. Silencing of another membrane protein gene preferentially expressed in R plants, Pectin methylesterase, previously shown to be involved in Tobacco mosaic virus translocation, did not lead to collapse of resistance of R plants. Thus, silencing of a single gene can lead to collapse of resistance, but not every gene preferentially expressed in the R line has the same effect, upon silencing, on resistance.

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Molecular Mapping of Ty-4, a New Tomato Yellow Leaf Curl Virus Resistance Locus on Chromosome 3 of Tomato

Cited by 136 publications

References 23 publications

Genetic control and mapping of Solanum chilense LA1932, LA1960 and LA1971-derived resistance to Tomato yellow leaf curl disease

Genetic control and mapping of Solanum chilense LA1932, LA1960 and LA1971-derived resistance to Tomato yellow leaf curl disease

Marker assisted selection of Ty-2 and Ty-3 carrying tomato lines and their implications in breeding tomato leaf curl disease resistant hybrids

Silencing of a single gene in tomato plants resistant to Tomato yellow leaf curl virus renders them susceptible to the virus

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