The 16S rDNAs from 38 strains of the genus Nocardioides, two Aeromicrobium species and Terrabacter tumescens were directly sequenced and then analysed. The mean nucleotide similarity value between the type strains of validly described Nocardioides species was 96.1 +/- 3.0%. The mean nucleotide similarity value between the type strains of validly described Nocardioides species and the two Aeromicrobium species was 93.7 +/- 1.4% T. tumescens was distantly related to the genera Nocardioides and Aeromicrobium. The mean intraspecific nucleotide similarity value of 16S rDNA sequences from Nocardioides albus was 99.5 +/- 0.5%. The mean intraspecific nucleotide similarity of 16S rDNA sequences from Nocardioides simplex was 100%, except for N. simplex strains ATCC 13260, ATCC 19565 and ATCC 19566, which were shown not to be members of the genus Nocardioides. 'Nocardioides flavus' strains IFO 14396T and IFO 14397, and 'Nocardioides fulvus' JCM 3335T showed a 16S rDNA similarity value of 100% with Nocardioides luteus KCTC 9575T and Nocardioides albus JCM 5854. 'N. fulvus' IFO 14399 shared its highest 16S rDNA similarity with Nocardioides sp. ATCC 39419 at 99%. 'N. fulvus' IFO 14399 and Nocardioides sp. ATCC 39419 formed a phylogenetic lineage distinct from the genera Nocardioides and Aeromicrobium. 'Nocardioides thermolilacinus' strains IFO 14335T and IFO 14336 displayed a close relationship to the genus Streptomyces. From 16S rDNA sequence analyses, it is considered that some strains that have been attributed to the genus Nocardioides should be taxonomically re-evaluated.

A light-orange-coloured, facultatively anaerobic, rod-shaped bacterium (strain SW28 T ), which was isolated from seawater in Korea, was taxonomically studied by a polyphasic approach. This organism formed round terminal endospores in swollen sporangia. The peptidoglycan type is A4α, based on L-Lys-L-Ala-D-Asp. The predominant menaquinone is MK-7 and the major fatty acid is ante-C 15 :0 . The GMC content of the DNA is 40 mol %. Phylogenetic analysis based on 16S rDNA sequences showed that strain SW28 T falls within the radiation of a cluster comprising the rRNA group 2 bacilli and non-Bacillus-type organisms. Strain SW28T showed the highest degree of relatedness to the type strain of Sporosarcina ureae, sharing 968 % 16S rDNA similarity. Levels of DNA-DNA relatedness between strain SW28 T and S. ureae DSM 2281 T and the type strains of some Bacillus species forming a coherent phylogenetic cluster are less than 125 %. On the basis of phenotypic and chemotaxonomic characteristics, 16S rDNA sequence analysis and DNA-DNA relatedness data, it is proposed that strain SW28T should be placed in the genus Sporosarcina as a new species, Sporosarcina aquimarina sp. nov. The type strain is SW28 T (l KCCM 41039 T l JCM 10887 T ). From the results of the taxonomic re-evaluation, it is also proposed that Bacillus globisporus, Bacillus psychrophilus and Bacillus pasteurii be transferred to the genus Sporosarcina as Sporosarcina globispora, Sporosarcina psychrophila and Sporosarcina pasteurii, respectively.

This paper presents the analysis of a surface-mounted permanent-magnet (SMPM) machine for the sensorless control scheme based on the high-frequency fluctuating voltage signal injection method. A simplified high-frequency model of an SMPM machine in the estimated rotor reference frame is developed and a sensorless rotor position and speed estimation algorithm is described. To support this, the high-frequency impedances of an SMPM machine are analyzed by finite-element analysis (FEA) and compared with measurement results using a pulsewidth-modulation (PWM) inverter system under various injection conditions. The results of the FEA and measurements are coincident with each other with some errors due to the nonlinear behavior of the PWM inverter and the SMPM machine. The analysis results give physical insights into selecting the injection conditions for sensorless operation of the SMPM machine even though adjustments considering nonlinear behaviors of PWM inverters are required in the actual operation for the desired performance. The experimental results of speed and position control using a commercial SMPM machine are presented based on the analysis of the SMPM machine for the sensorless control algorithm.Index Terms-High-frequency signal injection, sensorless operation, surface-mounted permanent-magnet (SMPM) machine. NOMENCLATURE SymbolsCoefficient of rotor position information signal (A). Equivalent proportional gain of a bang-bang controller in rotor position estimator. Proportional and integral gains of proportional-integral (PI) controller in rotor position estimator, respectively. Band of bang-bang controller. Resistance ( ).

The taxonomic status of four strains from several mineral water sources in Taejon, Korea, was examined. The DNA base compositions of these strains ranged from 62 to 66 mol %. All of the strains contained sphingolipids and ubiquinone 10 as the main respiratory quinone. The cellular fatty acids of these strains included octadecenoic acid, hexadecanoic acid and 2-hydroxymyristic acid, without 3-hydroxy fatty acids. On the basis of morphological, physiological and chemotaxonomic characteristics, together with 16S rDNA sequence comparison and DNA-DNA reassociation data, three new species of the genus Sphingomonas are proposed for the strains isolated from natural mineral water: Sphingomonas aquatilis sp. nov. for strain JSS7T (= KCTC 2881T = KCCM 41067T), Sphingomonas koreensis sp. nov. for strains JSS26T (= KCTC 2882T = KCCM 41069T) and JSS28 (= KCTC 2883 = KCCM 41066) and Sphingomonas taejonensis sp. nov. for strain JSS54T (= KCTC 2884T = KCCM 41068T).

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Two Gram-negative, non-motile, non-spore-forming, rod-shaped organisms, strains SW-125T and SW-154T, were isolated from tidal flat sediment of the Yellow Sea in Korea, and subjected to a polyphasic taxonomic study. Strains SW-125T and SW-154T grew optimally at 30–37 °C and in the presence of 2–3 % (w/v) NaCl. They contained ubiquinone-8 (Q-8) as the predominant respiratory lipoquinone and iso-C15 : 0 as the major fatty acid. The DNA G+C contents of strains SW-125T and SW-154T were 44 mol%. Phylogenetic trees based on 16S rRNA gene sequences revealed that the two strains form deep evolutionary lineages of descent within the γ-Proteobacteria. Strains SW-125T and SW-154T exhibited 16S rRNA gene sequence similarity levels of less than 90 % to members of the γ-Proteobacteria used in this analysis. Strains SW-125T and SW-154T showed a 16S rRNA gene sequence similarity level of 98·5 % and a mean DNA–DNA relatedness level of 9·4 %. Therefore, on the basis of phenotypic, phylogenetic and genomic data, a new genus, Kangiella gen. nov., is proposed to accommodate the novel strains, comprising two novel species, Kangiella koreensis sp. nov. (type strain, SW-125T=KCTC 12182T=DSM 16069T) and Kangiella aquimarina sp. nov. (type strain, SW-154T=KCTC 12183T=DSM 16071T).

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