Sphingomonas aquatilis sp. nov., Sphingomonas koreensis sp. nov., and Sphingomonas taejonensis sp. nov., yellow-pigmented bacteria isolated from natural mineral water.

Abstract

The taxonomic status of four strains from several mineral water sources in Taejon, Korea, was examined. The DNA base compositions of these strains ranged from 62 to 66 mol %. All of the strains contained sphingolipids and ubiquinone 10 as the main respiratory quinone. The cellular fatty acids of these strains included octadecenoic acid, hexadecanoic acid and 2-hydroxymyristic acid, without 3-hydroxy fatty acids. On the basis of morphological, physiological and chemotaxonomic characteristics, together with 16S rDNA sequence comparison and DNA-DNA reassociation data, three new species of the genus Sphingomonas are proposed for the strains isolated from natural mineral water: Sphingomonas aquatilis sp. nov. for strain JSS7T (= KCTC 2881T = KCCM 41067T), Sphingomonas koreensis sp. nov. for strains JSS26T (= KCTC 2882T = KCCM 41069T) and JSS28 (= KCTC 2883 = KCCM 41066) and Sphingomonas taejonensis sp. nov. for strain JSS54T (= KCTC 2884T = KCCM 41068T).

A Rare Case of Peritoneal Dialysis-Associated Peritonitis with Sphingomonas koreensis

“…There are a few cases of PD-associated peritonitis caused by Sphingomonas paucimobilis reported but none reported for Sphingomonas koreensis to date (4). The first evidence of Sphingomonas koreensis being a human pathogen was just recently published, in a case where it was the causative agent of meningitis in a patient (5). Sphingomonas koreensis is a gram-negative, rod-shaped, aerobic, yellow-pigmented bacterium with single polar flagella and derives its name from the fact that it was first isolated in natural mineral water in Korea in 2001 (6).…”

Proposal to reclassify [Sphingomonas] xenophaga Stolz et al. 2000 and [Sphingomonas] taejonensis Lee et al. 2001 as Sphingobium xenophagum comb. nov. and Sphingopyxis taejonensis comb. nov., respectively

“…The chemical properties of strains [Sphingomonas] xenophaga DSM 6383 T (Stolz et al, 2000) and [Sphingomonas] taejonensis DSM 15583 T (Lee et al, 2001), nitrate reduction, polyamine pattern and fatty acid profiling, also indicate that the strains show a strong associations with members of the genera Sphingobium and Sphingopyxis, respectively.…”

“…On the basis of our findings, we propose to reclassify [Sphingomonas] xenophaga Stolz et al 2000 and [Sphingomonas] taejonensis Lee et al 2001 as Sphingobium xenophagum comb. nov. and Sphingopyxis taejonensis comb.…”

“…The stability of the relationships was assessed by bootstrap analysis of 1000 datasets with SEQBOOT and CONSENSE programs in the PHYLIP package (Fig. 1) (Lee et al, 2001), the highest gene sequence similarities, of 98?7 and 98?2 %, were with Sphingopyxis chilensis DSM 14889 T (Godoy et al, 2003) and Sphingopyxis alaskensis DSM 13593 T (Vancanneyt et al, 2001), respectively. Additionally, the 16S rRNA gene sequences of [Sphingomonas] xenophaga DSM 6383 T (Stolz et al, 2000) and [Sphingomonas] taejonensis DSM 15583 T (Lee et al, 2001) contained the signature nucleotides specific for the genera Sphingobium cluster II and Sphingopyxis cluster IV , respectively, confirming that the strains should be reclassified in the genera Sphingobium and Sphingopyxis, respectively.…”

“…The almost complete 16S rRNA gene sequences for [Sphingomonas] xenophaga DSM 6383 T (1438 nt) (Stolz et al, 2000) and [Sphingomonas] taejonensis DSM 15583 T (1443 nt) (Lee et al, 2001), as well as the sequence of Rhodanobacter lindaniclasticus RP 5557 T (Nalin et al, 1999), were retrieved from the GenBank database at NCBI (http:// www.ncbi.nlm.nih.gov/). The 16S rRNA gene sequences of [Sphingomonas] xenophaga DSM 6383 T and [Sphingomonas] taejonensis DSM 15583 T were aligned with the 16S rRNA gene sequences of representative sphingomonads that encompass the four distinct sphingomonad genera by using CLUSTAL_X (version 1.8b; Thompson et al, 1997).…”