Virgibacillus koreensis sp. nov., a novel bacterium from a salt field, and transfer of Virgibacillus picturae to the genus Oceanobacillus as Oceanobacillus picturae comb. nov. with emended descriptions

Lee, Jung-Sook; Lim, Jee Min; Lee, Keun Chul; Lee, Jae-Chan; Park, Yong Ha; Kim, Chang‐Jin

doi:10.1099/ijs.0.63734-0

Cited by 106 publications

(80 citation statements)

References 35 publications

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“…The isoprenoid quinone of strain A1g T was MK-7 and the DNA G+C content was 36.3 mol%. These chemotaxonomic characteristics were in accordance with those given for the genus Oceanobacillus (Yumoto et al, 2005;Lee et al, 2006). Detailed results are given in the species description below and in Table 1. 16S rRNA gene sequence comparisons showed that strain A1g T should be placed within the genus Oceanobacillus, being related most closely to the type strains of O. oncorhynchi subsp.…”

supporting

confidence: 86%

“…The genus Oceanobacillus comprises aerobic, Gram-positive, motile, rod-shaped bacteria that are characterized chemotaxonomically by the presence of menaquinone-7 as the major isoprenoid quinone and anteiso-C 15 : 0 as the predominant cellular fatty acid (Lee et al, 2006).…”

mentioning

confidence: 99%

“…Members of the genus have been isolated from diverse sources such as deep-sea sediment (O. iheyensis Lu et al 2002;O. profundus Kim et al 2007), mural paintings [O. picturae (Heyrman et al 2003) Lee et al 2006], freshwater fish (O. oncorhynchi subsp. oncorhynchi Yumoto et al 2005), algae (O. oncorhynchi subsp.…”

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confidence: 99%

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Oceanobacillus neutriphilus sp. nov., isolated from activated sludge in a bioreactor

Yang

Huo

et al. 2010

International Journal of Systematic and Evolutionary Microbiology

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A Gram-stain-positive, neutrophilic, rod-shaped bacterium, strain A1g T , was isolated from activated sludge of a bioreactor and was subjected to a polyphasic taxonomic characterization. The isolate grew in the presence of 0-17.0 % (w/v) NaCl and at pH 6.0-9.0; optimum growth was observed in the presence of 3.0-5.0 % (w/v) NaCl and at pH 7.0. Strain A1g T was motile, formed cream-coloured colonies, was catalase-and oxidase-positive and was able to hydrolyse aesculin, Tween 40 and Tween 60. Chemotaxonomic analysis revealed menaquinone-7 as the predominant respiratory quinone and anteiso-C 15 : 0 , anteiso-C 17 : 0 , iso-C 16 : 0 and iso-C 15 : 0 as major fatty acids. The genomic DNA G+C content of strain A1g T was 36.3 mol%. Comparative 16S rRNA gene sequence analysis revealed that the new isolate belonged to the genus Oceanobacillus and exhibited closest phylogenetic affinity to the type strains of Oceanobacillus oncorhynchi subsp. incaldanensis (97.9 % similarity) and O. oncorhynchi subsp. oncorhynchi (97.5 %), but less than 97 % sequence similarity with respect to the type strains of other recognized Oceanobacillus species. Levels of DNA-DNA relatedness between strain A1g T and reference strains O. oncorhynchi subsp. incaldanensis DSM 16557 T , O. oncorhynchi subsp. oncorhynchi JCM 12661 T and Oceanobacillus iheyensis DSM 14371 T were 29, 45 and 38 %, respectively. On the basis of phenotypic and genotypic data, strain A1g T is considered to represent a novel species of the genus Oceanobacillus, for which the name Oceanobacillus neutriphilus sp. nov. is proposed. The type strain is A1g T (5CGMCC 1.7693 T 5JCM 15776 T ).

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confidence: 86%

mentioning

confidence: 99%

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Oceanobacillus neutriphilus sp. nov., isolated from activated sludge in a bioreactor

Yang

Huo

et al. 2010

International Journal of Systematic and Evolutionary Microbiology

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“…nov., within the family Bacillaceae. Additionally, this study shows that two species, Virgibacillus koreensis (Lee et al, 2006) and Virgibacillus albus (Zhang et al, 2012), which do not fall within the cluster of the genus Virgibacillus, are very closely related to strain B6B T and, therefore, we propose the transfer of these two species to the new genus Aquibacillus, as Aquibacillus koreensis comb. nov. and Aquibacillus albus comb.…”

mentioning

confidence: 77%

“…The presence of anteiso-C 15 : 0 as the major fatty acid was similar to phylogenetically closely related taxa, while the pattern for other predominant fatty acids of strain B6B T was different from those of V. koreensis IBRC-M 10657 T , V. albus IBRC-M 10798 T and Sediminibacillus halophilus DSM 18088 T (Table S1). Lee et al (2006). DData taken from Zhang et al (2012).…”

Section: Ornithinibacillus Bavariensis Wsbc 24001 T (Y13066) Ornithinmentioning

confidence: 99%

Aquibacillus halophilus gen. nov., sp. nov., a moderately halophilic bacterium from a hypersaline lake, and reclassification of Virgibacillus koreensis as Aquibacillus koreensis comb. nov. and Virgibacillus albus as Aquibacillus albus comb. nov.

Amoozegar

Bagheri

Didari

et al. 2014

International Journal of Systematic and Evolutionary Microbiology

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Aquibacillus halophilus gen. nov., sp. nov., a moderately halophilic bacterium from a hypersaline lake, and reclassification of Virgibacillus koreensis as Aquibacillus koreensis comb. nov. and Virgibacillus albus as Aquibacillus albus comb. nov. A novel Gram-stain-positive, moderately halophilic bacterium, designated strain B6B T , was isolated from the water of an Iranian hypersaline lake, Aran-Bidgol, and characterized taxonomically using a polyphasic approach. Cells of strain B6B T were rod-shaped, motile and produced ellipsoidal endospores in terminal positions in non-swollen sporangia. Strain B6B T was a strictly aerobic bacterium and catalase-and oxidase-positive. The strain was able to grow at NaCl concentrations of 0.5-20.0 % (w/v), with optimum growth occurring at 10.0 % (w/v) NaCl. The optimum temperature and pH for growth were 35 6C and pH 7.0. On the basis of 16S rRNA gene sequence analysis, strain B6B T was shown to belong to the phylum Firmicutes and its closest phylogenetic similarities were with the species Virgibacillus koreensis BH30097 T (97.5 %), Virgibacillus albus YIM 93624 T (97.4 %), Sediminibacillus halophilus EN8d T (96.8 %), Sediminibacillus albus NHBX5 T (96.6 %), Virgibacillus carmonensis LMG 20964 T (96.3 %) and Paraliobacillus quinghaiensis YIM-C158 T (96.0 %), respectively. Phylogenetic analysis revealed that strain B6B T , along with V. koreensis BH30097 T and V. albus YIM 93624 T , clustered in a separate clade in the family Bacillaceae. The DNA G+C content of the novel isolate was 35.8 mol%. DNA-DNA hybridization experiments revealed low levels of relatedness between strain B6B T and V. koreensis BH30097 T (13 %) and V. albus YIM 93624 T (33 %). The major cellular fatty acid of strain B6B T was anteiso-C 15 : 0 (75.1 %) and its polar lipid pattern consisted of phosphatidylglycerol, diphosphatidylglycerol, an unknown phospholipid and an unknown glycolipid. The isoprenoid quinones were MK-7 (90 %) and MK-6 (3 %). The peptidoglycan contained meso-diaminopimelic acid as the diagnostic diamino acid. All of these features support the placement of isolate B6B T within the phylum Firmicutes. It is closely related to V. koreensis and V. albus, but with features that clearly distinguish it from species of the genus Virgibacillus or of other related genera. On the basis of the polyphasic evidence derived in this study, we propose that strain B6B T be placed within a new genus, as Aquibacillus halophilus gen. nov., sp. nov., with B6B T as the type strain (5IBRC-M 10775 T 5KCTC 13828 T ). We also propose that V. koreensisOne supplementary table and three supplementary figures are available with the online version of this paper.The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain B6B T is HQ433456.

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Lentibacillus

Heyrman¹,

Vos²

2015

Bergey's Manual of Systematics of Archaea and Bacteria

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Len.ti.ba.cil'lus. L. adj. lentus slow; L. dim. n. bacillus small rod; N.L. masc. n. Lentibacillus slowly growing bacillus/rod. Firmicutes / “Bacilli” / Bacillales / Bacillaceae / Lentibacillus Rod‐shaped cells, forming terminal endospores that swell the sporangia. Gram‐variable, motile or nonmotile. Colonies are white to cream‐colored, smooth and circular to slightly irregular. Catalase‐positive, oxidase variable, and urease‐negative. Unable to hydrolyze starch, tyrosine, or xanthine. No acid production from D ‐melibiose, raffinose, or L ‐rhamnose. Moderately to extremely halophilic. The major fatty acid is C 15 : 0 anteiso and branched saturated fatty acids account for 95% total fatty acids . The cell‐wall peptidoglycan contains meso ‐diaminopimelic acid at position 3 of the peptide subunit. The predominant menaquinone is MK‐7. The major polar lipids are diphosphatidylglycerol and phosphatidylglycerol. DNA G + C content ( mol %): 42.0–44.0. Type species : Lentibacillus salicampi Yoon, Kang and Park 2002, 2047 VP .

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Virgibacillus koreensis sp. nov., a novel bacterium from a salt field, and transfer of Virgibacillus picturae to the genus Oceanobacillus as Oceanobacillus picturae comb. nov. with emended descriptions

Cited by 106 publications

References 35 publications

Oceanobacillus neutriphilus sp. nov., isolated from activated sludge in a bioreactor

Oceanobacillus neutriphilus sp. nov., isolated from activated sludge in a bioreactor

Aquibacillus halophilus gen. nov., sp. nov., a moderately halophilic bacterium from a hypersaline lake, and reclassification of Virgibacillus koreensis as Aquibacillus koreensis comb. nov. and Virgibacillus albus as Aquibacillus albus comb. nov.

Lentibacillus

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