Oceanobacillus neutriphilus sp. nov., isolated from activated sludge in a bioreactor

Abstract

A Gram-stain-positive, neutrophilic, rod-shaped bacterium, strain A1g T , was isolated from activated sludge of a bioreactor and was subjected to a polyphasic taxonomic characterization. The isolate grew in the presence of 0-17.0 % (w/v) NaCl and at pH 6.0-9.0; optimum growth was observed in the presence of 3.0-5.0 % (w/v) NaCl and at pH 7.0. Strain A1g T was motile, formed cream-coloured colonies, was catalase-and oxidase-positive and was able to hydrolyse aesculin, Tween 40 and Tween 60. Chemotaxonomic analysis revealed menaquinone-7 as the predominant respiratory quinone and anteiso-C 15 : 0 , anteiso-C 17 : 0 , iso-C 16 : 0 and iso-C 15 : 0 as major fatty acids. The genomic DNA G+C content of strain A1g T was 36.3 mol%. Comparative 16S rRNA gene sequence analysis revealed that the new isolate belonged to the genus Oceanobacillus and exhibited closest phylogenetic affinity to the type strains of Oceanobacillus oncorhynchi subsp. incaldanensis (97.9 % similarity) and O. oncorhynchi subsp. oncorhynchi (97.5 %), but less than 97 % sequence similarity with respect to the type strains of other recognized Oceanobacillus species. Levels of DNA-DNA relatedness between strain A1g T and reference strains O. oncorhynchi subsp. incaldanensis DSM 16557 T , O. oncorhynchi subsp. oncorhynchi JCM 12661 T and Oceanobacillus iheyensis DSM 14371 T were 29, 45 and 38 %, respectively. On the basis of phenotypic and genotypic data, strain A1g T is considered to represent a novel species of the genus Oceanobacillus, for which the name Oceanobacillus neutriphilus sp. nov. is proposed. The type strain is A1g T (5CGMCC 1.7693 T 5JCM 15776 T ).

Oceanobacillus chungangensis sp. nov., isolated from a sand dune

“…Overall, the results obtained in this study are in agreement with previously published data for the 11 recognized species of the genus Oceanobacillus (Lu et al, 2001;Heyrman et al, 2003;Lee et al, 2006Lee et al, , 2010Kim et al, 2007;Raats and Halpern 2007;Nam et al, 2008;Namwong et al, 2009;Tominaga et al, 2009;Yang et al, 2010;Whon et al, 2010 T was harvested from tryptic soy agar (TSA; Difco) after cultivation for 3 days at 30 u C. The physiological age of biomass of all the strains for fatty acid analysis was standardized by observing growth profiles of cultures and harvesting according to a standard MIDI protocol (Sherlock Microbial Identification System, version 6.1). Cellular fatty acid methyl esters (FAMEs) were obtained as described by Minnikin et al (1980), and separated by an automated gas chromatography system (model 6890N and 7683 autosampler; Agilent).…”

“…The genus Oceanobacillus comprises aerobic, Gram-positive, motile, rod-shaped bacteria that are characterized chemotaxonomically by the presence of MK-7 as the major isoprenoid quinone and anteiso-C 15 : 0 as the predominant cellular fatty acid (Namwong et al, 2009;Lee et al, 2010;Whon et al, 2010). At the time of writing, the genus harbours 11 characterized species with validly published names: O. iheyensis (Lu et al, 2001), O. picturae (Heyrman et al, 2003), O. oncorhynchi (Yumoto et al, 2005), O. chironomi (Raats & Halpern, 2007), O. profundus (Kim et al, 2007), O. caeni (Nam et al, 2008), O. kapialis (Namwong et al, 2009), O. sojae (Tominaga et al, 2009), O. neutriphilus (Yang et al, 2010), O. locisalsi (Lee et al, 2010) and O. kimchii (Whon et al, 2010) (http://www. bacterio.net/).…”

Oceanobacillus luteolus sp. nov., isolated from soil

“…As for the type species Oceanobacillus iheyensis DSM 14371 T , the predominant fatty acid is variable in different reports, iso-C 15 : 0 in this study and by Kim et al (2007), but anteiso-C 15 : 0 by Hirota et al (2013a). The DNA G+C content of the two novel strains was 38.6-39.2 mol%, which is in accordance with values for the genus Oceanobacillus (35.8-40.1 mol%; Yang et al, 2010). Both strains contained meso-diaminopimelic acid (meso-DAP) as the diamino acid, and this is the same with other species of the genus Oceanobacillus (Hirota et al, 2013a).…”

Oceanobacillus damuensis sp. nov. and Oceanobacillus rekensis sp. nov., isolated from saline alkali soil samples

“…Members of Oceanobacillus have been isolated from deep-sea sediment, chironomid egg mass, sludge, soil, salt lake, marine solar saltern, sand dune, marine sand, the skin of a rainbow trout, algal mat, painting, wastewater-treatment system, fermented shrimp paste, fermented food, soy sauce production equipment, and indigo fermentation fluid. At the time of this writing, eighteen Oceanobacillus species have been described: O. iheyensis (Lu et al 2001), O. picturae (Heyrman et al 2003;Lee et al 2006), O. oncorhynchi (including two subspecies; Yumoto et al 2005), O. chironomi (Raats and Halpern 2007), O. profundus (Kim et al 2007), O. caeni (Nam et al 2008), O. kapialis (Namwong et al 2009), O. sojae (Tominaga et al 2009), O. locisalsi (Lee et al 2010), O. neutriphilus (Yang et al 2010), O. kimchii (Whon et al 2010), O. chungangensis (Lee et al 2013), O. indicireducens (Hirota et al 2013a, b), O. polygoni (Hirota et al 2013a, b), O. limi (Amoozegar et al 2014), O. luteolus (Wu et al 2014), O. pacificus and O. arenosus (Kim et al 2015).…”