fr/). Members of the genusMaribacter have been isolated from various marine habitats such as green algae, red algae, seawater and marine sediment (Nedashkovskaya and Kim, 2011). In the course of the screening of bacteria with biotechnological potential from marine samples, a bacterial strain, designated CAU 1044 T , was isolated from a seaweed (Undaria pinnatifida) sample collected in Jeju Island (33 u 229 47.300 N 126 u 329 59.320 E) in the Republic of Korea. The purpose of the present study was to establish the taxonomic position of this bacterial strain by using polyphasic characterization.Isolation was performed according to the protocol of Gordon & Mihm (1962) ). The crushed sample was diluted with sterilized saline solution. Serial dilutions were spread onto MA plates and a single colony was subcultivated under aerobic conditions at 30 u C for 3 days. Pure cultures were preserved at 270 u C in marine broth (MB; Difco) supplemented with 25 % (v/v) glycerol. M. arcticus KCTC 22053 T was obtained from the Korean Collection for type Cultures (KCTC; Taejon, Korea) and used as a reference strain in most analyses.Genomic DNA of strain CAU 1044 T was isolated by the method of Marmur (1961). The 16S rRNA gene was amplified by PCR following established procedures (Nam et al., 2004). The amplified 16S rRNA gene was sequenced directly using a BigDye Terminator Cycle Sequencing kit (Applied Biosystems) and an automatic DNA sequencer (model 3730; Applied Biosystems). Multiple alignments with sequences of the type strains of the ten species of the genus Maribacter and calculation of sequence similarity levels were carried out by using the EzTaxon server (http:// Abbreviation: TEM, transmission electron microscopy.The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain CAU 1044T is JN036550.

A Gram-stain-positive, spore-forming, rod-shaped, motile, strictly aerobic bacterial strain, designated CAU 1051 T , was isolated from a sand dune and its taxonomic position was investigated using a polyphasic approach. Strain CAU 1051T grew optimally at pH 5.0 and 30 6C. NaCl was not required for growth but up to 10.0 % (w/v) NaCl was tolerated.

A Gram-stain-negative, non-spore-forming, non-motile, strictly aerobic, rod-shaped bacterial strain, designated CAU 1002 T , was isolated from a tidal flat sediment and its taxonomic position was investigated using a polyphasic approach. Strain CAU 1002 T grew optimally at 30 6C and pH 7.5. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain CAU 1002 T formed a distinct lineage within the genus Algoriphagus and was most closely related to Algoriphagus lutimaris KCTC 22630 T and Algoriphagus halophilus KCTC 12051 T (97.75 and 97.74 % 16S rRNA gene sequence similarity, respectively). The strain contained MK-7 as the major isoprenoid quinone and iso-C 15 : 0 and C 16 : 1 v7c and/or iso-C 15 : 0 2-OH (summed feature 3) as the major fatty acids. The cell-wall peptidoglycan of strain CAU 1002 T contained mesodiaminopimelic acids. The major whole-cell sugars were glucose, arabinose, sucrose, and ribose. The polar lipid profile was composed of phosphatidylethanolamine, five unidentified aminolipids, one unidentified aminophospholipid, one unidentified phospholipid, one unidentified aminoglycolipid, one unidentified glycolipid and twelve unidentified lipids. The DNA G+C content of strain CAU 1002 T was 38.0 mol%. On the basis of phylogenetic inference, phenotypic, chemotaxonomic and genotypic data, strain CAU 1002 T should be classified into the genus Algoriphagus as a member of a novel species, for which the name Algoriphagus chungangensis sp. nov. is proposed. The type strain is CAU 1002 T (5KCTC 23759 T 5CCUG 61890 T ). The description of the genus Algoriphagus is emended.The genus Algoriphagus, a member of the phylum Bacteroidetes, was described by Bowman et al. (2003). At the time of writing, this genus comprised 19 species with validly published names (http://www.bacterio.cict.fr/). Recently, members of the genus Algoriphagus have been isolated from various environmental habitats such as freshwater (Liu et al., 2009;Rau et al., 2012), oilcontaminated soil (Young et al., 2009), a tidal flat sediment (Park et al., 2010) and seawater (Oh et al., 2012). Members of this genus are Gram-negative, rod-shaped, non-motile, non-sporulating, and possess menaquinone-7 (MK-7) as the major respiratory quinone and iso-C 15 : 0 as the predominant cellular fatty acid (Yoon et al., 2006). In the course of screening bacteria with biotechnological potential from marine samples, a bacterial strain, designated CAU 1002 T , was isolated from a tidal flat sediment sample collected from Sido Island (37 u 329 13.050 N 126 u 259 07.240 E) in the Yellow Sea, Republic of Korea. The purpose of the present study was to establish the taxonomic position of this bacterial strain by using a polyphasic approach that included the determination of phenotypic and chemotaxonomic properties, a detailed phylogenetic investigation based on 16S rRNA gene sequences and genetic analysis.The procedure for isolation of the strain followed that of Gordon & Mihm (1962) using marine agar 2216 (MA; Difco), supplemented with cycloheximide (50 m...

A Gram-stain-positive, spore-forming, rod-shaped, motile, strictly aerobic bacterial strain, designated CAU 1033 T , was isolated from soil and its taxonomic position was investigated using a polyphasic approach. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain CAU 1033 T formed a distinct lineage within the genus Bacillus and was most closely related to Bacillus drentensis KCTC 13025 T (similarity 95.9 %). CAU 1033 T contained MK-7 as the only isoprenoid quinone and iso-C 15 : 0 and anteiso-C 15 : 0 as the major fatty acids. The cell wall peptidoglycan of strain CAU 1033 T contained meso-diaminopimelic acid and the major whole-cell sugars were arabinose, sucrose and ribose. The polar lipids were composed of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, two unidentified phospholipids, four unidentified aminophospholipids, an unidentified aminolipid, two unidentified glycolipids and another unidentified polar lipid. The DNA G+C content was 41.4 mol%. On the basis of phenotypic data and phylogenetic inference, strain CAU 1033 T was classified as a representative of a novel species in the genus Bacillus for which the name Bacillus songklensis sp. nov. is proposed. The type strain is CAU 1033 T (5KCTC 13881 T 5CCUG 61889 T ).

A Gram-stain-negative, aerobic, motile, short rod-shaped, catalase-negative and oxidase-positive bacterium, strain CAU 1568T, was isolated from marine sediment sand sampled at Sido Island in the Republic of Korea. The optimum conditions for growth were at 25–30 °C, at pH 6.5–8.5 and with 0–4.0 % (w/v) NaCl. Phylogenetic analysis based on the 16S rRNA gene sequence indicated that strain CAU 1568T was a member of the genus Photobacterium with high similarity to Photobacterium salinisoli JCM 30852T (97.7 %), Photobacterium halotolerans KACC 17089T (97.3 %) and Photobacterium galatheae LMG F28894T (97.3 %). The predominant cellular fatty acids were C16 : 0, summed feature 3 (C16 : 1  ω6c and/or C16 : 1  ω7c) and summed feature 8 (C18 : 1  ω7c and/or C18 : 1  ω6c), with Q-8 as the major of isoprenoid quinone. The polar lipid profile consisted of diphosphatidylglycerol, phosphatidylglycerols, phosphatidylcholine, phosphatidylethanolamine, phospholipid, two aminophospholipids and three unidentified lipids. The whole genome size of strain CAU 1568T was 4.8 Mb with 50.1 mol% G+C content; including 38 contigs and 4233 protein-coding genes. These taxonomic data support CAU 1568T as representing a novel Photobacterium species, for which the name Photobacterium arenosum sp. nov. is proposed. The type strain of this novel species is CAU 1568T (=KCTC 82404T=MCCC 1K05668T).