Maribacter chungangensis sp. nov., isolated from a green seaweed, and emended descriptions of the genus Maribacter and Maribacter arcticus

Abstract

fr/). Members of the genusMaribacter have been isolated from various marine habitats such as green algae, red algae, seawater and marine sediment (Nedashkovskaya and Kim, 2011). In the course of the screening of bacteria with biotechnological potential from marine samples, a bacterial strain, designated CAU 1044 T , was isolated from a seaweed (Undaria pinnatifida) sample collected in Jeju Island (33 u 229 47.300 N 126 u 329 59.320 E) in the Republic of Korea. The purpose of the present study was to establish the taxonomic position of this bacterial strain by using polyphasic characterization.Isolation was performed according to the protocol of Gordon & Mihm (1962) ). The crushed sample was diluted with sterilized saline solution. Serial dilutions were spread onto MA plates and a single colony was subcultivated under aerobic conditions at 30 u C for 3 days. Pure cultures were preserved at 270 u C in marine broth (MB; Difco) supplemented with 25 % (v/v) glycerol. M. arcticus KCTC 22053 T was obtained from the Korean Collection for type Cultures (KCTC; Taejon, Korea) and used as a reference strain in most analyses.Genomic DNA of strain CAU 1044 T was isolated by the method of Marmur (1961). The 16S rRNA gene was amplified by PCR following established procedures (Nam et al., 2004). The amplified 16S rRNA gene was sequenced directly using a BigDye Terminator Cycle Sequencing kit (Applied Biosystems) and an automatic DNA sequencer (model 3730; Applied Biosystems). Multiple alignments with sequences of the type strains of the ten species of the genus Maribacter and calculation of sequence similarity levels were carried out by using the EzTaxon server (http:// Abbreviation: TEM, transmission electron microscopy.The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain CAU 1044T is JN036550.

Maribacter luteus sp. nov., a marine bacterium isolated from intertidal sand of the Yellow Sea

“…At the time of writing, the genus comprises 23 species with validly published names. All members of the genus Maribacter were isolated from diverse marine environments, such as seawater [2,3], marine sediment [4], red alga [5], seaweed [6] and sponge [7]. The genus Maribacter is generally characterized to be Gram-stain-negative, aerobic or facultatively anaerobic, motile by gliding or non-motile.…”

“…The major or only respiratory quinone is menaquinone 6 (MK-6), and the major fatty acids are straight-chain saturated, branched-chain saturated and unsaturated fatty acids iso-C 15 : 0 , iso-C 15 : 1 , C 15 : 0 and iso-C 17 : 0 3-OH. The genomic DNA G+C contents are in the range of 32.6-41.1 % [1,2,6]. In this study, a novel bacterial strain, designated RZ05 T , was isolated and determined using a polyphasic taxonomic approach.…”

Morphogenesis of Ulva mutabilis (Chlorophyta) induced by Maribacter species (Bacteroidetes, Flavobacteriaceae)

“…Indeed, Maribacter chungangensis (Weerawongwiwat et al 2013), Maribacter stanieri (Nedashkovskaya et al 2010) and Maribacter ulvicola (Nedashkovskaya et al 2004a), isolated from green seaweed, and Maribacter arcticus (Cho et al 2008) and Maribacter sedimenticola (Nedashkovskaya et al 2004a), isolated from marine sediment, all displayed similar morphogenetic activities (Figure 2A-E). Importantly, in combination with Roseovarius sp.…”

“…Maribacter are gram-negative, rod shaped cells, which produce non-diffusible yellow to orange pigments. The metabolism is aerobic or facultatively anaerobic (Nedashkovskaya et al 2004a, Barbeyron et al 2008, Lo et al 2013, Weerawongwiwat et al 2013, Hu et al 2015, Jackson et al 2015. They were found in various marine sources and climatic zones (Cho et al 2008, Zhang et al 2009, Tang et al 2015 and could be isolated from the water column (Yoon et al 2005, Barbeyron et al 2008) and the sea sediment (Nedashkovskaya et al 2004a, Cho et al 2008.…”

“…They were found in various marine sources and climatic zones (Cho et al 2008, Zhang et al 2009, Tang et al 2015 and could be isolated from the water column (Yoon et al 2005, Barbeyron et al 2008) and the sea sediment (Nedashkovskaya et al 2004a, Cho et al 2008. Maribacter strains are often associated with the surface of macroalgae such as Ulva fenestra Postels & Ruprecht (Nedashkovskaya et al 2004a, Weerawongwiwat et al 2013, U. mutabilis (Spoerner et al 2012) and Polysiphonia (Nedashkovskaya et al 2007), where they harbour various enzyme activities involved in degradation of, for example, algal polysaccharides (Bakunina et al 2012).…”