Algoriphagus chungangensis sp. nov., isolated from a tidal flat sediment

Abstract

A Gram-stain-negative, non-spore-forming, non-motile, strictly aerobic, rod-shaped bacterial strain, designated CAU 1002 T , was isolated from a tidal flat sediment and its taxonomic position was investigated using a polyphasic approach. Strain CAU 1002 T grew optimally at 30 6C and pH 7.5. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain CAU 1002 T formed a distinct lineage within the genus Algoriphagus and was most closely related to Algoriphagus lutimaris KCTC 22630 T and Algoriphagus halophilus KCTC 12051 T (97.75 and 97.74 % 16S rRNA gene sequence similarity, respectively). The strain contained MK-7 as the major isoprenoid quinone and iso-C 15 : 0 and C 16 : 1 v7c and/or iso-C 15 : 0 2-OH (summed feature 3) as the major fatty acids. The cell-wall peptidoglycan of strain CAU 1002 T contained mesodiaminopimelic acids. The major whole-cell sugars were glucose, arabinose, sucrose, and ribose. The polar lipid profile was composed of phosphatidylethanolamine, five unidentified aminolipids, one unidentified aminophospholipid, one unidentified phospholipid, one unidentified aminoglycolipid, one unidentified glycolipid and twelve unidentified lipids. The DNA G+C content of strain CAU 1002 T was 38.0 mol%. On the basis of phylogenetic inference, phenotypic, chemotaxonomic and genotypic data, strain CAU 1002 T should be classified into the genus Algoriphagus as a member of a novel species, for which the name Algoriphagus chungangensis sp. nov. is proposed. The type strain is CAU 1002 T (5KCTC 23759 T 5CCUG 61890 T ). The description of the genus Algoriphagus is emended.The genus Algoriphagus, a member of the phylum Bacteroidetes, was described by Bowman et al. (2003). At the time of writing, this genus comprised 19 species with validly published names (http://www.bacterio.cict.fr/). Recently, members of the genus Algoriphagus have been isolated from various environmental habitats such as freshwater (Liu et al., 2009;Rau et al., 2012), oilcontaminated soil (Young et al., 2009), a tidal flat sediment (Park et al., 2010) and seawater (Oh et al., 2012). Members of this genus are Gram-negative, rod-shaped, non-motile, non-sporulating, and possess menaquinone-7 (MK-7) as the major respiratory quinone and iso-C 15 : 0 as the predominant cellular fatty acid (Yoon et al., 2006). In the course of screening bacteria with biotechnological potential from marine samples, a bacterial strain, designated CAU 1002 T , was isolated from a tidal flat sediment sample collected from Sido Island (37 u 329 13.050 N 126 u 259 07.240 E) in the Yellow Sea, Republic of Korea. The purpose of the present study was to establish the taxonomic position of this bacterial strain by using a polyphasic approach that included the determination of phenotypic and chemotaxonomic properties, a detailed phylogenetic investigation based on 16S rRNA gene sequences and genetic analysis.The procedure for isolation of the strain followed that of Gordon & Mihm (1962) using marine agar 2216 (MA; Difco), supplemented with cycloheximide (50 m...

Algoriphagus aestuarii sp. nov., a member of the Cyclobacteriaceae isolated from a tidal-flat sediment of the Yellow Sea in Korea

“…Strain MDM-1 T exhibited 16S rRNA gene sequence similarity of 98.5, 98.2, 97.4 and 97.2 % to A. halophilus KCTC 12051 T , A. lutimaris S1-3 T , A. machipongonensis DSM 24695 T and A. chungangensis KCTC 23759 T , respectively, 92.8–97.6 % similarity to the type strains of other species of the genus Algoriphagus and less than 91.5 % similarity to the other strains used in the phylogenetic analysis. The predominant isoprenoid quinone detected in strain MDM-1 T was menaquinone 7 (MK-7), which is compatible with that of A. halophilus , A. lutimaris , A. machipongonensis and A. chungangensis (Nedashkovskaya et al , 2004; Park et al , 2010; Alegado et al , 2013; Kang et al , 2013). The cellular fatty acid profile of strain MDM-1 T is shown in , together with those of the type strains of five phylogenetically closely related species of the genus Algoriphagus grown and analysed under identical conditions.…”

“…The genus Algoriphagus , a member of the family Cyclobacteriaceae , was proposed by Bowman et al (2003) to accommodate a Gram-negative, catalase-negative, oxidase-positive, small coccobacillus, Algoriphagus ratkowskyi . At the time of writing, the genus Algoriphagus was represented by 28 species with validly published names, isolated from various environments, including algae (Nedashkovskaya et al , 2004), a freshwater pipe (Rau et al , 2012), lakes (Van Trappen et al , 2004; Liu et al , 2009), seawater (Lee et al , 2012; Oh et al , 2012), tidal-flat sediments (Park et al , 2010; Li et al , 2011; Kang et al , 2013; Kim et al , 2014; Park et al , 2014) and soil (Yoon et al , 2006; Young et al , 2009). In this study, a novel bacterial strain, designated MDM-1 T , which was isolated from a tidal-flat sediment, is described.…”

“… * Data taken from: a , Nedashkovskaya et al (2004); b , Park et al (2010); c , Alegado et al (2013); d , Kang et al (2013); e , Bowman et al (2003). …”

Algoriphagus confluentis sp. nov., isolated from the junction between the ocean and a freshwater lake

“…The isoprenoid quinones were eluted by a mixture of methanol/2-propanol (2 : 1, v/v) using a flow rate of 1 ml min 21 at room temperature and detected by UV absorbance at 270 nm. The predominant isoprenoid quinone detected in strain HJM-2 T was menaquinone-7 (MK-7) in line with the genus Algoriphagus (Nedashkovskaya et al, , 2007Kang et al, 2013;Park et al, 2014). For cellular fatty acid analysis, cell mass of strain HJM-2 T was harvested from MA plates after cultivation for 3, 5 and 7 days at 30 8C and cell mass of A. taiwanensis JCM 19755 T was harvested from MA plates after cultivation for 5 days at 30 8C.…”

Algoriphagus taeanensis sp. nov., isolated from a tidal flat, and emended description of Algoriphagus hitonicola

“…The genus Algoriphagus , a member of the family Cyclobacteriaceae , was proposed by Bowman et al (2003). Members of the genus are Gram-staining-negative, non-motile and rod-shaped and have iso-C 15 : 0 as the major fatty acid (Kang et al , 2013). At the time of writing, the genus comprises 26 species (http://www.bacterio.net/a/algoriphagus.html).…”