Algoriphagus aestuarii sp. nov., a member of the Cyclobacteriaceae isolated from a tidal-flat sediment of the Yellow Sea in Korea

Abstract

A Gram-strain-negative, coccoid or oval-shaped, non-motile bacterial strain, designated MDM-1 T , was isolated from a tidal-flat sediment on the Korean peninsula. Strain MDM-1 T was found to grow optimally at pH 7.0-8.0, at 30 8C and in the presence of 2-3 % (w/v) NaCl. A neighbour-joining phylogenetic tree based on 16S rRNA gene sequences showed that strain MDM-1 T falls within the clade comprising species of the genus Algoriphagus, clustering with the type strains of Algoriphagus halophilus, A. lutimaris, A. chungangensis and A. machipongonensis, with which it exhibited 97.2-98.5 % 16S rRNA gene sequence similarity. Sequence similarities to the type strains of the other recognized species of the genus Algoriphagus were 92.8-97.6 %. Strain MDM-1 T was found to contain MK-7 as the predominant menaquinone and iso-C 15 : 0 and summed feature 3 (C 16 : 1 v6c and/or C 16 : 1 v7c) as the major fatty acids. The major polar lipids were identified as phosphatidylcholine, phosphatidylethanolamine and two unidentified lipids. The DNA G+C content of strain MDM-1 T was determined to be 42.7 mol% and the mean DNA-DNA relatedness with A. halophilus KCTC 12051 T , A. lutimaris S1-3 T , A. chungangensis KCTC 23759 T , A. machipongonensis DSM 24695 T and A. ratkowskyi CIP 107452 T was 19.7-5.2 %. Differential phenotypic properties, together with the phylogenetic and genetic distinctiveness, revealed that strain MDM-1 T is distinguishable from recognized species of the genus Algoriphagus. On the basis of the data presented, strain MDM-1 T is proposed to represent a novel species of the genus Algoriphagus, for which the name Algoriphagus aestuarii sp. nov. is proposed. The type strain is MDM-1 T (5KCTC 42199 T 5NBRC 110552 T).

Algoriphagus pacificus sp. nov. and Algoriphagus oliviformis sp. nov., isolated from a mariculture fishpond

“…YJ13C T and H41 T contained MK-7 as the major respiratory quinone, similar to the respiratory quinone profiles of their close neighbours [36][37][38][39][40]. The cellular fatty acid profiles of strains YJ13C T , A. halophilus KCTC 12051 T , A. lutimaris KCTC 22630 T , A. aestuarii KCTC 42199 T and A. iocasae CCTCC AB 2015446 T were a little different from those of strains H41 T , A. terrigena KCTC 12545 T and A. aestuariicola JCM 31546 T .…”

“…YJ13C T and H41 T contained MK-7 as the major respiratory quinone, similar to the respiratory quinone profiles of their close neighbours [36–40]. The cellular fatty acid profiles of strains YJ13C T , A.…”

“…The polar lipids profiles of both YJ13C T and H41 T consisted of phosphatidylethanolamine and several kinds of unidentified lipids, that were similar to the polar lipids profiles of their close neighbours (Fig. S7) [36–40]. Phosphatidylethanolamine was the major polar lipid of species of the genus Algoriphagus and some unknown lipids also occurred in each polar lipids profile of species of the genus Algoriphagus , but the concentrations of these unknown lipids were lower.…”