Oceanobacillus damuensis sp. nov. and Oceanobacillus rekensis sp. nov., isolated from saline alkali soil samples

Long, Xiufeng; Ye, Renyuan; Zhang, Shuai; Liu, Bo; Zhang, Yuqin; Zeng, Zhigang; Tian, Yongqiang

doi:10.1007/s10482-015-0529-9

Cited by 19 publications

(4 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“… f Claus and Berkeley ( 29 ). g Long et al (data were not from type species) ( 30 ). h Yoon et al ( 31 ).…”

Section: Resultsmentioning

confidence: 99%

Novel spore-forming species exhibiting intrinsic resistance to third- and fourth-generation cephalosporins and description of Tigheibacillus jepli gen. nov., sp. nov.

Miliotis,

Sengupta,

Hameed

et al. 2024

mBio

View full text Add to dashboard Cite

A comprehensive microbial surveillance was conducted at NASA’s Mars 2020 spacecraft assembly facility (SAF), where whole-genome sequencing (WGS) of 110 bacterial strains was performed. One isolate, designated 179-BFC-A-HS T , exhibited less than 80% average nucleotide identity (ANI) to known species, suggesting a novel organism. This strain demonstrated high-level resistance [minimum inhibitory concentration (MIC) >256 mg/L] to third-generation cephalosporins, including ceftazidime, cefpodoxime, combination ceftazidime/avibactam, and the fourth-generation cephalosporin cefepime. The results of a comparative genomic analysis revealed that 179-BFC-A-HS T is most closely related to Virgibacillus halophilus 5B73C T , sharing an ANI of 78.7% and a digital DNA-DNA hybridization (dDDH) value of 23.5%, while their 16S rRNA gene sequences shared 97.7% nucleotide identity. Based on these results and the recent recognition that the genus Virgibacillus is polyphyletic, strain 179-BFC-A-HS T is proposed as a novel species of a novel genus, Tigheibacillus jepli gen. nov., sp. nov (type strain 179-BFC-A-HS T = DSM 115946 T = NRRL B-65666 T ), and its closest neighbor, V. halophilus , is proposed to be reassigned to this genus as Tigheibacillus halophilus comb. nov. (type strain 5B73C T = DSM 21623 T = JCM 21758 T = KCTC 13935 T ). It was also necessary to reclassify its second closest neighbor Virgibacillus soli, as a member of a novel genus Paracerasibacillus , reflecting its phylogenetic position relative to the genus Cerasibacillus , for which we propose Paracerasibacillus soli comb. nov. (type strain CC-YMP-6 T = DSM 22952 T = CCM 7714 T ). Within Amphibacillaceae ( n = 64), P. soli exhibited 11 antibiotic resistance genes (ARG), while T. jepli encoded for 3, lacking any known β-lactamases, suggesting resistance from variant penicillin-binding proteins, disrupting cephalosporin efficacy. P. soli was highly resistant to azithromycin (MIC >64 mg/L) yet susceptible to cephalosporins and penicillins. IMPORTANCE The significance of this research extends to understanding microbial survival and adaptation in oligotrophic environments, such as those found in SAF. Whole-genome sequencing of several strains isolated from Mars 2020 mission assembly cleanroom facilities, including the discovery of the novel species Tigheibacillus jepli , highlights the resilience and antimicrobial resistance (AMR) in clinically relevant antibiotic classes of microbes in nutrient-scarce settings. The study also redefines the taxonomic classifications within the Amphibacillaceae family, aligning genetic identities with phylogenetic data. Investigating ARG and virulence factors (VF) across these strains illuminates the microbial capability for resistance under resource-limited conditions while emphasizing the role of human-associated VF in microbial survival, informing sterilization practices and microbial management in similar oligotrophic settings beyond spacecraft assembly cleanrooms such as pharmaceutical and medical industry cleanrooms.

show abstract

“… f Claus and Berkeley ( 29 ). g Long et al (data were not from type species) ( 30 ). h Yoon et al ( 31 ).…”

Section: Resultsmentioning

confidence: 99%

Novel spore-forming species exhibiting intrinsic resistance to third- and fourth-generation cephalosporins and description of Tigheibacillus jepli gen. nov., sp. nov.

Miliotis,

Sengupta,

Hameed

et al. 2024

mBio

View full text Add to dashboard Cite

show abstract

“…At the time of writing, this genus comprises 21 validly named species, including six recently described species, Oceanobacillus limi (Amoozegar et al 2014), Oceanobacillus luteolus (Wu et al 2014), Oceanobacillus arenosus (Kim et al 2015), Oceanobacillus bengalensis (Yongchang et al 2015), Oceanobacillus rekensis and Oceanobacillus damuensis (Long et al 2015). Members of the genus Oceanobacillus have been isolated from a wide variety of environments.…”

Section: Introductionmentioning

confidence: 99%

Oceanobacillus endoradicis sp. nov., an endophytic bacterial species isolated from the root of Paris polyphylla Smith var. yunnanensis

Yang

Tang

Chu

et al. 2016

Antonie van Leeuwenhoek

View full text Add to dashboard Cite

A bacterial strain, py1294(T), isolated from a root of Paris polyphylla Smith var. yunnanensis collected from Yunnan province, southwest China, was characterised by using a polyphasic approach to clarify its taxonomic position. Strain py1294(T) was found to be Gram-positive, aerobic, spore-forming, peritrichous flagella and rod shaped. Growth was found to occur in the presence of 0-8 % (w/v) NaCl (optimum 1-3 %), at pH 6.5-9.5 (optimum 8.0) and at 10-42 °C (optimum 30 °C). The major cellular fatty acids were identified as anteiso-C15:0, anteiso-C17:0, iso-C16:0 and iso-C14:0. The predominant quinone was identified as MK-7 and a minor amount of MK-6 was detected. The diagnostic polar lipids were diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine. The cell wall peptidoglycan was found to contain meso-diaminopimelic acid. Phylogenetic analysis of the 16S rRNA gene sequence showed that strain py1294(T) forms a well-supported clade with Oceanobacillus damuensis PT-20(T) (97.9 % sequence similarity) within the genus Oceanobacillus, although it also shares a high sequence similarity with Ornithinibacillus contaminans (97.5 %). Crucially, the DNA-DNA relatedness value between strain py1294(T) and O. damuensis PT-20(T) was 29.7 ± 3.2 %. The G+C content was determined to be 42.3 mol%. On the basis of the phylogenetic and phenotypic data, a novel species Oceanobacillus endoradicis sp. nov. is proposed, with py1294(T) (=DSM 100726(T) = KCTC 33731(T)) as the type strain.

show abstract

“…So far, there are 17 validly named species in this genus according to List of Prokaryotic Names with Standing in Nomenclature (http://www. bacterio.net/oceanobacillus.html, Lu et al 2001;Heyrman et al 2003;Yumoto et al 2005;Romano et al 2006;Kim et al 2007; Raats and Halpern 2007;Nam et al 2008;Namwong et al 2009;Tominaga et al 2009;Lee et al 2010;Yang et al 2010;Whon et al 2010;Hirota et al 2013a, b;Lee et al 2013;Amoozegar et al 2014;Wu et al 2014a, b;Yu et al 2014;Kim et al 2015); in addition there are also 7 species Roux et al 2013;Jang et al 2014;Liu and Yang 2014;Tang et al 2014;Long et al 2015) with effectively published names. Bacteria included in this genus and those in the genus Ornithinibacillus (Mayr et al 2006) are closely related.…”

Section: Introductionmentioning

confidence: 99%

Oceanobacillus bengalensis sp. nov., a bacterium isolated from seawater of the Bay of Bengal

Ouyang

Xiang

Wang

2015

Antonie van Leeuwenhoek

View full text Add to dashboard Cite

A Gram-stain positive, motile, and subterminal endospore-forming rod-shaped bacterium, designated strain Ma-21(T), was isolated from seawater of the Bay of Bengal. Strain Ma-21(T) was found to grow optimally at 37 °C and pH 8.0 with 3% (w/v) NaCl. Phylogenetic analyses showed that strain Ma-21(T) forms a distinct phylogenetic lineage close to Oceanobacillus chungangensis CAU 1051(T), Oceanobacillus caeni S-11(T), Oceanobacillus arenosus CAU 1183(T), Oceanobacillus halophilum GD01(T) and Ornithinibacillus heyuanensis GIESS003(T) in the family Bacillaceae. The cell wall of strain Ma-21(T) was found to contain meso-diaminopimelic acid as the diagnostic diamino acid, which is in line with those of members of the genus Oceanobacillus. The genomic DNA G+C content was determined to be 35.9 mol%. The only respiratory quinone detected was MK-7. The major cellular fatty acids were identified as anteiso-C(15:0) and anteiso-C(17:0). The major polar lipids were found to be diphosphatidylglycerol and phosphatidylglycerol. On the basis of phylogenetic, chemotaxonomic and phenotypic properties, strain Ma-21(T) is suggested to represent a novel species in the genus Oceanobacillus, for which the name Oceanobacillus bengalensis sp. nov. is proposed. The type strain is Ma-21(T) (=CGMCC 1.12799(T) = KCTC 33416(T) = MCCC 1K00260(T)).

show abstract

Oceanobacillus damuensis sp. nov. and Oceanobacillus rekensis sp. nov., isolated from saline alkali soil samples

Cited by 19 publications

References 37 publications

Novel spore-forming species exhibiting intrinsic resistance to third- and fourth-generation cephalosporins and description of Tigheibacillus jepli gen. nov., sp. nov.

Novel spore-forming species exhibiting intrinsic resistance to third- and fourth-generation cephalosporins and description of Tigheibacillus jepli gen. nov., sp. nov.

Oceanobacillus endoradicis sp. nov., an endophytic bacterial species isolated from the root of Paris polyphylla Smith var. yunnanensis

Oceanobacillus bengalensis sp. nov., a bacterium isolated from seawater of the Bay of Bengal

Contact Info

Product

Resources

About