A moderately halophilic bacterium, strain HNA-14 T , was isolated from a saline-alkali soil sample collected in Shache County, Xinjiang Province. On the basis of the polyphasic taxonomic data, the isolate was considered to be a member of the genus Bacillus. The organism grew optimally at 30 C and pH 8.0. It was moderately halophilic and its optimum growth occurred at 5 10% NaCl. The diamino acid found in the cell-wall peptidoglycan was meso-diaminopimelic acid and the predominant menaquinone was MK-7. The major cellular fatty acids were anteiso-C 15:0 and iso- IntroductionThe genus Bacillus was first described by Cohn in 1872. Early in the history of microbiology, members of the Bacillus genus (266 species) were found ubiquitously, and it is one of the genera with the largest 16S diversity and environmental diversity (Logan et al., 2007). Many halophilic Bacilli were isolated from a wide range of habitats recently, such as the intestinal tract of an earthworm (Hong et al., 2012), foods (Seiler et al., 2012), a hypersaline lake (Bagheri et al., 2012), soybean root (Zhang et al., 2012) and non-saline forest soil (Chen et al., 2011c). In this paper, we report on taxonomic characterization of a moderately halophilic, Gram-stainingpositive and spore-forming bacterial strain, HNA-14 T , which was isolated from a saline-alkali soil sample collected in Shache County, Xinjiang Province, north-west China. On the basis of phenotypic characteristics, chemotaxonomic data, phylogenetic analysis and 16S rRNA data, the isolate is considered to represent a novel species of the genus Bacillus. Materials and MethodsStrains and culture conditions. Strain HNA-14 T was isolated from a saline-alkali soil sample collected in Shache County, Xinjiang Province by the serial dilution plating Full PaperBacillus shacheensis sp. nov., a moderately halophilic bacterium isolated from a saline-alkali soil (Received December 20, 2013; Accepted April 3, 2014) Zuchao Lei,
Polygonum cuspidatum Sieb. et Zucc. is a traditional Chinese medicinal plant that produces polydatin, a glycosylated derivative of resveratrol. Thus far, 244 prokaryotic endophytes have been isolated and identified from P. cuspidatum using 16s rDNA sequence. The results show that the endophytic bacteria in P. cuspidatum belong to five orders, namely, Actinomycetales, Bacillales, Rhizobiales, Pseudomonadales, and Enterobacteriales. At the genus level, 244 strains were identified, including Lysinibacillus,
Two moderately halophilic strains, PT-11(T) and PT-20(T), were isolated from saline alkali soil samples collected in Shache County, Xinjiang Province, China. Both strains are aerobic, Gram-positive, motile rods. Strain PT-11(T) grows at 15-40 °C and at pH 6.5-10.0, while PT-20(T) grows at 15-40 °C and at pH 6.5-11.0. The major cellular fatty acids in both strains include anteiso-C15:0, anteiso-C17:0 and iso-C15:0. For both strains, the polar lipids consist of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, an unidentified phospholipid and several unidentified lipids. In addition, strain PT-20(T) also contains phosphatidylcholine. The major isoprenoid quinone for both strains is MK-7. The genomic G+C content is 36.7 % for PT-11(T) and 39.2 % for PT-20(T). Phylogenetic analyses of 16S rRNA gene sequences indicated that these two isolates are members of the genus Oceanobacillus. DNA-DNA hybridization indicated that strains PT-11(T) and PT-20(T) should be considered two distinct species. On the basis of both phylogenetic and chemotaxonomic data analyses, therefore, we conclude that PT-11(T) and PT-20(T) represent two novel species within the genus Oceanobacillus, for which we propose the names Oceanobacillus rekensis sp. nov. and Oceanobacillus damuensis sp. nov., respectively. The type strains are PT-11(T) (=KCTC 33144(T) = DSM 26900(T)) and PT-20(T) (=KCTC 33146(T) = DSM 26901(T)).
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