A moderately halophilic bacterium, strain HNA-14 T , was isolated from a saline-alkali soil sample collected in Shache County, Xinjiang Province. On the basis of the polyphasic taxonomic data, the isolate was considered to be a member of the genus Bacillus. The organism grew optimally at 30 C and pH 8.0. It was moderately halophilic and its optimum growth occurred at 5 10% NaCl. The diamino acid found in the cell-wall peptidoglycan was meso-diaminopimelic acid and the predominant menaquinone was MK-7. The major cellular fatty acids were anteiso-C 15:0 and iso- IntroductionThe genus Bacillus was first described by Cohn in 1872. Early in the history of microbiology, members of the Bacillus genus (266 species) were found ubiquitously, and it is one of the genera with the largest 16S diversity and environmental diversity (Logan et al., 2007). Many halophilic Bacilli were isolated from a wide range of habitats recently, such as the intestinal tract of an earthworm (Hong et al., 2012), foods (Seiler et al., 2012), a hypersaline lake (Bagheri et al., 2012), soybean root (Zhang et al., 2012) and non-saline forest soil (Chen et al., 2011c). In this paper, we report on taxonomic characterization of a moderately halophilic, Gram-stainingpositive and spore-forming bacterial strain, HNA-14 T , which was isolated from a saline-alkali soil sample collected in Shache County, Xinjiang Province, north-west China. On the basis of phenotypic characteristics, chemotaxonomic data, phylogenetic analysis and 16S rRNA data, the isolate is considered to represent a novel species of the genus Bacillus. Materials and MethodsStrains and culture conditions. Strain HNA-14 T was isolated from a saline-alkali soil sample collected in Shache County, Xinjiang Province by the serial dilution plating Full PaperBacillus shacheensis sp. nov., a moderately halophilic bacterium isolated from a saline-alkali soil (Received December 20, 2013; Accepted April 3, 2014) Zuchao Lei,
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