The reduction of CH4 from the digestive tract of ruminants can be done through the use of organic components of plants such as tannins and saponins and the use of probiotics. This study aims to evaluate the addition of organic components and probiotics to the characteristics of rumen fluid and its ability to reduce CH4 in Ongole Cross Breed (PO) cattle. Sengon (Paraserianthes falcataria) leaf meal and Trembesi (Samanea saman) leaf meal are used as organic components due to their tannin and saponin content. Probiotics contain Acetoanaerobium notarae and Saccharomyces cereviseae. This research used total mixed ration as a feed. A total of 24 heads PO cattle were divided into 4 treatments, ie T1 = control treatment; T2 = T1 + organic components, T3 = T1 + Probiotics and T4 = T1 + organic components + probiotics. The research design was a randomized block design. The combination treatment of the addition of organic components and probiotic caused a decrease in the ratio of acetic acid to propionic acid (C 2 :C3), percentage of acetic acid, the concentration of CO2 and CH4, but increases the percentage of propionate acid. The combination of organic components and probiotics is the greatest decrease in CH4 production from enteric fermentation.

About 25% of the anthropogenic methane emissions are due to ruminal fermentation from ruminant livestock. Green medicated supplement (Green MS) is a prototype herb and slow-release urea-based supplement to reduce methane emissions from livestock. This experiment aimed to evaluate the methane emission and digestibility of forage-based rations supplemented with Green MS. This study examines six forages (palm oil leaves, rice straw, Napier grass, sugarcane leaves, native grass and maize straw). That six forages were supplemented with Green MS. The total treatments were 12, with four replications. A completely randomized design was applied in this study. Besides native grass, Green MS can reduce in vitro enteric methane by 0.23 - 3.05 ml/200 mg DM (P < 0.05). However, Green MS did not change the value of all forage’s optimum gas production (a+b). It is interesting to note that gas production from non-soluble fibre (GPNSF) could be enhanced with Green MS in maize straw and native grass (P < 0.05). The response to supplements varies depending on the utilization of the feed substrate in incubation. In conclusion, without affecting feed digestibility, Green MS supplementation could lower enteric methane emissions.

Background and Aim: Among several factors, the sperm quality of poultry is affected by the rooster's body size and the availability of antioxidants like vitamin E. This study aimed to determine the effect of dietary vitamin E supplementation on rooster sperm quality through a meta-analysis. Materials and Methods: After verification and evaluation, a total of 19 articles were included in this study. Data, including dietary vitamin E, semen volume, concentration, total sperm cells, pH, motility, viability, percentage of dead and abnormal sperm, vitamin E sperm content, malondialdehyde (MDA) content, and testosterone levels, were tabulated in a database; these were subsequently analyzed using mixed modeling with vitamin E dose as a fixed effect and study identity as a random effect. Results: Dietary supplementation level of vitamin E significantly (p<0.001) affected sperm concentration, significantly affected motility (p<0.001), significantly affected sperm vitamin E (p<0.001), significantly affected viability (p<0.001), and significantly affected chicken sperm fertility (p=0.001). Vitamin E administration also significantly reduced the number of sperm cell deaths (p<0.001); however, increased dietary levels of vitamin E did not affect semen volume (p=0.853), pH (p=0.951), MDA (p=0.542), the percentage of abnormal sperm cells (p=0.343), nor testosterone levels (p=0.063). Conclusion: Dietary vitamin E supplementation is recommended for male chickens since it generally enhances the quality of their sperm.

This research aims to know the influence of the farmer breeder characteristics on the business scale which measured by livestock number owned. This research was conducted in the city of Probolinggo, East Java. The respondents in this study included beef cattle farmer breeders who are the active members of Bumi Karomah group. The number of respondents were 30 peoples. Data were collected through interviews using a questionnaire. Data in this research were analyzed using multiple regression analysis to test the influence of beef cattle farmer breeder characteristics on business scale. Multiple linear regression analysis result showed that age, number of family burden and cattle raising experience do not affect significantly to business scale owned by farmer breeders with determination coefficient 17% means that the characteristics of breeders on in family burden variables affects the number of business scale only by 17%, and the rest is affected by other variables. Meaning, that the diversity of the three characteristics of the breeder are not strong enough to explain the diversity of the business scale.

The objective of this study was to evaluate the yield, nutrient profile and in vitro digestibility of new BMR mutant lines of sorghum in Indonesia. These mutant lines were GH2.1, GH2.2, GH2.3, GH4.1, GH4.2, GH4.3 and GH4.4. One sorghum mutant line (CTY) and two national sorghum varieties (Super 1 and Bioguma) were also evaluated as controls. In vitro digestibility and rumen fermentation were measured using Ankom Daisy Fermenter and Hohenheim gas test methods, respectively. In vitro measurement consisted of ten treatments with five replications following a completely randomized design. The highest stem sugar content was found in Bioguma (11.22%) and GH4.4 (9.32%) (p<0.05). The Bioguma variety and the GH2.3 mutant line had a higher number of stem segments and fresh forage yield than the Super 1 variety (p<0.05). A greater concentration of crude protein (CP) was observed for the GH.2.1, GH2.2, GH2.3 and GH4.1 lines (p<0.05). The GH2.3 mutant line had the lowest acid detergent lignin (ADL) content (p<0.05), while Bioguma had the highest level of non-fibre carbohydrate (NFC) compounds (p<0.05). The highest relative feed value (RFV) was observed for the GH2.3 line (p<0.05). Furthermore, GH4.2 and GH2.3 had greater in vitro true digestibility (IVTD) (p<0.05) but were not significantly different from Bioguma. Regarding yield characteristics, nutrient composition and in vitro digestibility values, the highest values were found in the Bioguma variety and the GH2.3 mutant line. Except for n-valerate (nC5), significant differences in all rumen fermentation parameters were observed among sorghum cultivars (p<0.05). Regarding the interrelationship between parameters, we found a medium correlation of DMD with the ADL and cellulose content of sorghum forage (R 2 = -0.489 and R 2 = -0.674, respectively). Based on these findings, the GH2.3 BMR mutant line should be further developed as forage sorghum.

Synchronization of feed sources of energy and protein can produce a positive influence on microbial protein synthesis, especially in ruminants that are fed low quality feed such as corn waste. Corn waste, including corn straw and corn peri carp are widely used as beef cattle feed. The objective of this research was to determine the effect of synchronizing the rate degradation of protein and energy of feed base on corn waste on rumen fermentation characteristic and synthesis protein microbial on 24 hours in vitro condition. The three synchronization index levels in corn-based feed waste are 0.4 (low); 0.5 (moderate) and 0.6 (high) were used in this study. Characteristics of rumen fermentation observed were pH, individual volatile fatty acids (acetic acid, propionic acid and butyric acid), NH3, C02, CH4, degradation of organic matter, microbial protein production, and efficiency of microbial protein synthesis. Increasing the synchronization index level caused a significant increase (P<0.05) of NH3 (53.60 to 59.07 mg/L) and efficiency of microbial protein synthesis (14.83 to 17.87) , but high synchronization index decrease (P<0.05) the concentration of acetic acid (22.45 mM to 15.95 mM), estimated CO2 (18.69 to 15.50 mol) and CH4 (11.49 to 7.98) in rumen fluid. This result indicated that the improvement of synchronization index can improve the productivity of livestock through increase efficiency of microbial protein synthesis and it reduce global warming.

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