Parasit cacing (Helminth) merupakan kendala kesehatan yang berhubungan dengan investasi parasit sering dijumpai pada ayam. Penelitian ini bertujuan untuk mengidentifikasi keragaman parasit cacing yang terdapat pada ayam kampung di Kabupaten Jember. Prosedur pengambilan sampel ekskreta ayam kampung dilakukan secara acak. Sampel ekskreta yang diambil sebanyak 150 sampel di Kabupaten Jember. Identifikasi keragaman spesies cacing dilakukan melalui pemeriksaan telur cacing pada sampel ekskreta dengan metode sedimentasi yang dilakukan di BBVet (Balai Besar Veteriner) Wates, Yogyakarta. Parasit cacing yang teridentifikasi pada sampel ekskreta ayam kampung merupakan cacing dari kelas Nematoda yang terdiri dari Capillaria sp. (24,67%); Heterakis sp. (10,67%); Strongyloides sp. (3,33%); dan Ascaridia sp. (2,67%).
Fasciola gigantica diagnosis usually performed by detection of worm eggs presence in the feces, but this conventional method has many disadvantages. Early diagnosis (early detection) cannot be performed in conventional methods because the worms in the host's body began to lay eggs at the age of 8-12 weeks of patency. The current detection method that is based on antibodyantigen reactions using excreted/secreted (ES) liquid by adult F. gigantica, is believed to be used for the early detection of fasciolosis. This study aimed to characterize the antigenic components of F. gigantica extretory/secretory products that could be used as a vaccine candidate development for early fasciolosis diagnostics. ES products were separated by PEG4000 at various concentrations (8%, 16%, 24%), then precipitates (pellets) obtained were dialyzed and characterized using SDSPAGE and Western blotting. Results from SDS PAGE showed that there were 18 proteins bands with 7-70 kDa molecular weights. Western blotting on pellets derived from PEG separation at various concentrations affirmed that the proteins of 50, 25 and 20 kDa were antigenic at 8% PEG concentration, the 25 kDa and 50 kDa were antigenic at 16% PEG concentrations and the 25 kDa was antigenic at 25% PEG concentration.
Antibiotics are the most widely used feed additives in broiler feed, but dependence on antibiotics has now begun to be reduced when it is known that antibiotics use has produced harmful residuals on both livestock and human health. The study produced synbiotics from the fungus Trichoderma sp. developed in local materials such as cassava and then encapsulated using skim milk and dextrousmalto. Design research in this study experimental design using a factorial Completely Randomized Design (CRD) pattern of three replications. The research concluded most excellent concentration of dried cassava flour was 100% and supplemented with 4% Trichoderma koningii. The greatest number of synbiotics spores 416.2, pH ranged 4.40-5.80, glucose test 0.30%. Result of proximate analysis synbiotics, which have already been encapsulated using maltodkestrin and skim milk contain dry matter 89.11%, crude protein 3.21%, Crude fat 0.73%, Crude fiber 2.87% and Ash 1.25%. The inhibition of encapsulated synbiotics to Salmonella sp shows that the resulted synbiotics would be encapsulated using ratio maltodextrin:skim is 1:10.
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