Hepatocellular carcinoma (HCC) as a harmful malady that truly debilitates human health, it is significant to isolate and on-line profile the phenotype of HCC cells for further diagnosis and therapy....
Temperature
variation at the nanoscale is pivotal for the thermodynamics
and kinetics of small entities. Surface-enhanced Raman spectroscopy
(SERS) is a promising technique for monitoring temperature variations
at the nanoscale. A key but ambiguous topic is methods to design a
sensitive SERS thermometer. Here, we elucidate that the type of chemical
bond of molecular probes and the surface chemical bonding effect are
crucial for maximizing the sensitivity of the SERS thermometer, as
illustrated by the variable-temperature SERS measurements and quantum
chemistry calculations for the frequency–temperature functions
of a series of molecules. The sensitivity of the frequency–temperature
function follows the sequence of triple bond > double bond >
single
bond, which is available for both aliphatic and aromatic molecules.
The surface chemical bonding effect between the SERS substrate and
molecular probe substantially increases the sensitivity of the frequency–temperature
function. These results provide universally available guidelines for
the rational design of a sensitive SERS thermometer by examining the
functional groups of molecular probes.
We developed a new plasmonic nanostripe microcone array
(PNMA)
substrate-integrated microfluidic chip for the simultaneous surface-enhanced
Raman scattering (SERS)-based immunoassay of the creatine kinase MB
isoenzyme (CK-MB) and cardiac troponin (cTnI) cardiac markers. The
conventional immunoassay usually employs a microtiter plate as the
solid capture plate to form the immunocomplexes. However, the two-dimensional
(2D) surface of the microtiter plate limits the capture efficiency
of the target antigens due to the steric hindrance effect. To address
this issue, a gold film-coated microcone array with nanostripes was
developed that can provide a large surface area for capture antibody
conjugation and serve as a SERS-active substrate. This unique nano–microhierarchical
structure showed an excellent light trapping effect and induced surface
plasmon resonance to further enhance the Raman signals of the SERS
nanoprobes. It significantly improved the sensitivity and applicability
of SERS-based immunoassay on the microfluidic chip. With this integrated
microfluidic chip, we successfully performed the simultaneous detection
of CK-MB and cTnI, and the detection limit can reach 0.01 ng mL–1. It is believed that the PNMA substrate-integrated
microfluidic chip would play a critical role in the rapid and sensitive
diagnostics of cardiac diseases.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.