Simultaneous single-cell phenotype analysis of hepatocellular carcinoma CTCs using a SERS-aptamer based microfluidic chip

Gao, Rongke; Zhan, Changbiao; Wu, Chun-Yu; Lu, Yang; Cao, Baoqiang; Huang, Jing; Wang, Feng; Yu, Liandong

doi:10.1039/d1lc00516b

Cited by 35 publications

(21 citation statements)

References 56 publications

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“…The manufacturing procedure adopts rapid prototyping technology and UV lithography technology . Briefly, a negative SU-8 2100 photoresist mold was processed on a silicon wafer using a high-resolution photomask on the mask aligner (MDA-400M, MIDAS, Korea).…”

Section: Methodsmentioning

confidence: 99%

“…The manufacturing procedure adopts rapid prototyping technology and UV lithography technology. 42 Briefly, a negative SU-8 2100 photoresist mold was processed on a silicon wafer using a highresolution photomask on the mask aligner (MDA-400M, MIDAS, Korea). As the PDMS prepolymer and the curing agent were mixed in a ratio of 10:1 (w/w), the bubbles were removed using a vacuum pump.…”

Section: Design and Fabrication Of The Integrated Microfluidic Chipmentioning

confidence: 99%

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SERS-Based Immunoassay of Myocardial Infarction Biomarkers on a Microfluidic Chip with Plasmonic Nanostripe Microcones

Gao

Mao

et al. 2022

ACS Appl. Mater. Interfaces

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We developed a new plasmonic nanostripe microcone array (PNMA) substrate-integrated microfluidic chip for the simultaneous surface-enhanced Raman scattering (SERS)-based immunoassay of the creatine kinase MB isoenzyme (CK-MB) and cardiac troponin (cTnI) cardiac markers. The conventional immunoassay usually employs a microtiter plate as the solid capture plate to form the immunocomplexes. However, the two-dimensional (2D) surface of the microtiter plate limits the capture efficiency of the target antigens due to the steric hindrance effect. To address this issue, a gold film-coated microcone array with nanostripes was developed that can provide a large surface area for capture antibody conjugation and serve as a SERS-active substrate. This unique nano–microhierarchical structure showed an excellent light trapping effect and induced surface plasmon resonance to further enhance the Raman signals of the SERS nanoprobes. It significantly improved the sensitivity and applicability of SERS-based immunoassay on the microfluidic chip. With this integrated microfluidic chip, we successfully performed the simultaneous detection of CK-MB and cTnI, and the detection limit can reach 0.01 ng mL–1. It is believed that the PNMA substrate-integrated microfluidic chip would play a critical role in the rapid and sensitive diagnostics of cardiac diseases.

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Section: Methodsmentioning

confidence: 99%

Section: Design and Fabrication Of The Integrated Microfluidic Chipmentioning

confidence: 99%

SERS-Based Immunoassay of Myocardial Infarction Biomarkers on a Microfluidic Chip with Plasmonic Nanostripe Microcones

Gao

Mao

et al. 2022

ACS Appl. Mater. Interfaces

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“…For example, based on the SERS aptamer, Gao et al. [ 103 ] developed a microfluidic chip for in situ single-cell phenotyping by characterizing the expression levels of surface biomarkers of CTCs. ( Fig.…”

Section: Aptamer-based Microfluidics For Ctc Detectionmentioning

confidence: 99%

Recent progress in aptamer-based microfluidics for the detection of circulating tumor cells and extracellular vesicles

Sun

et al. 2023

Journal of Pharmaceutical Analysis

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“…In previous studies, the Raman technique was used to capture hepatic carcinoma cells from whole blood samples and to sort Escherichia coli from aqueous samples. 18 , 19 Device-based dielectrophoresis (DEP) can be used to isolate bone marrow-derived mesenchymal stem cells from a heterogeneous cell mixture and to select monocytes from whole blood samples. 20 , 21 Acoustic techniques have been used to separate U87 cancer cells from red blood cells and to sort prostate cancer cells from whole blood samples.…”

Section: Introductionmentioning

confidence: 99%

Separation and single-cell analysis for free gastric cancer cells in ascites and peritoneal lavages based on microfluidic chips

Zhao¹,

Han²,

Xu³

et al. 2023

eBioMedicine

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Simultaneous single-cell phenotype analysis of hepatocellular carcinoma CTCs using a SERS-aptamer based microfluidic chip

Abstract: Hepatocellular carcinoma (HCC) as a harmful malady that truly debilitates human health, it is significant to isolate and on-line profile the phenotype of HCC cells for further diagnosis and therapy....

Cited by 35 publications

References 56 publications

SERS-Based Immunoassay of Myocardial Infarction Biomarkers on a Microfluidic Chip with Plasmonic Nanostripe Microcones

SERS-Based Immunoassay of Myocardial Infarction Biomarkers on a Microfluidic Chip with Plasmonic Nanostripe Microcones

Recent progress in aptamer-based microfluidics for the detection of circulating tumor cells and extracellular vesicles

Separation and single-cell analysis for free gastric cancer cells in ascites and peritoneal lavages based on microfluidic chips

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