Rapid simultaneous SERS detection of dual myocardial biomarkers on single-track finger-pump microfluidic chip

Liu, Yiyuan; Gao, Rongke; Zhuo, Ying; Wang, Yeru; Jia, Huakun; Chen, Xiaozhe; Lu, Yang; Zhang, Dongzhi; Yu, Liandong

doi:10.1016/j.aca.2022.340673

Cited by 19 publications

(12 citation statements)

References 36 publications

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“…Recently, different types of nanomaterial-based PoCT devices have been developed and utilized various optical read-out techniques: Ps-AuNPs-Aptamer-BSA microparticles label-free microfluidic paper-based colorimetric sensing device (μ-PAD) for cTnT via smartphone-based image analysis; red fluorescent-based PoC LFIA systems for the detection of cTnI; QBs@SiO 2 -COOH/ quantum-dot nanobeads LFIA colorimetric assay system for cTnI; functionalized gold chip-based PoC-SPR biosensor for the detection of NT-proBNP; Nile blue tagged Ag/AuNPs core/shell-based SERS LFA strip for multiplex detection of cardiac biomarkers of Myo, cTnI, and CK-MB in real samples; malachite green isothiocyanate tagged with AuNPs@anti-cTnI and Nile blue tagged with AuNPs@anti-MB immunoassays-based finger-pump microfluidic chip for simultaneous SERS detection of cTnI and CK-MB …”

Section: Discussionmentioning

confidence: 99%

“…Yu et al demonstrated a SERS-based finger-pump microfluidic chip for simultaneous detection of cTnI and CK-MB using two types of immunoassays such as malachite green isothiocyanate tagged with AuNPs@anti-cTnI and Nile blue tagged with AuNPs@anti-MB. 240 The finger-pump microfluidic chip contains three parts: (i) three inlets for sample flow injection, (ii) detection chamber for collecting the immunocomplexes using a round magnet, and (iii) finger-pump which is seen in Figure 11f. Antibodies conjugated SERS nanoprobes to form "sandwich" immunocomplexes of cTnI and CK-MB, and the LOD was 0.01 ng/mL.…”

Section: Point-of-care Testing (Poct) For Cardiac Biomarkersmentioning

confidence: 99%

Section: Point-of-care Testing (Poct) For Cardiac Biomarkersmentioning

confidence: 99%

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Optical Nanobiosensor-Based Point-of-Care Testing for Cardiovascular Disease Biomarkers

Vairaperumal,

Huang,

Liu

2023

ACS Appl. Bio Mater.

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Cardiovascular disease is a global health threat, and detecting cardiac biomarkers is essential for early-stage diagnosis and personalized treatment. Traditional approaches have limitations, but optical nanobiosensors offer rapid, highly selective, and sensitive detection. Optical nanobiosensors generate biosignals that transfer light signals while analytes bind with the bioreceptors. Optical nanobiosensors have advantages such as ease of monitoring, low cost, a wide detection range, and high sensitivity without any interference. An optical nanobiosensor platform is a promising approach for point-of-care cardiac biomarker detection with a low detection limit. This review mainly focuses on the detection of cardiovascular disease biomarkers based on various optical nanobiosensor approaches that have been reported during the last five years, and we have categorized them based on optical signal readouts. A detailed discussion of the classification of cardiovascular disease biomarkers, design strategies of optical biosensors, types of optically active nanomaterials, types of bioreceptors, functionalization techniques, various assay types, and sensing mechanisms is presented. Then, we summarize the optical signaling readout-based various nanobiosensors systems for the detection of cardiovascular disease biomarkers. Finally, we summarize and conclude with the recent development of point-of-care testing (PoCT) for cardiovascular disease biomarkers used in various optical readout techniques.

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Section: Discussionmentioning

confidence: 99%

Section: Point-of-care Testing (Poct) For Cardiac Biomarkersmentioning

confidence: 99%

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Optical Nanobiosensor-Based Point-of-Care Testing for Cardiovascular Disease Biomarkers

Vairaperumal,

Huang,

Liu

2023

ACS Appl. Bio Mater.

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“…It is worth noting that these techniques may address some challenges such as complex design strategies, variability among users, the requirement of an external operator, and complicated micro-valving setups often require extensive microfabrication and assembly processes. [50][51][52] Additive manufacturing (3D printing) techniques can substantially address the challenges associated with microfabrication and with microfluidic handling on a large scale. 53,54 Indeed AM can bring about outcomes comparable to traditional lithography in terms of temporal and spatial resolution, material, and mechanical properties while being cost-effective, rapid, and scalable.…”

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Additive manufacturing leveraged microfluidic setup for sample to answer colorimetric detection of pathogens

Yedire,

Hosseini,

Shieh

et al. 2023

Lab Chip

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One‐Stop Plasmonic Nanocube‐Excited SERS Immunoassay Platform of Multiple Cardiac Biomarkers for Rapid Screening and Progressive Tracing of Acute Myocardial Infarction

Lee,

Kim,

Song

et al. 2023

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Rapid and precise acute myocardial infarction (AMI) diagnosis is essential for preventing patient death. In addition, the complementary roles of creatine kinase muscle brain (CK‐MB) and cardiac troponin I (cTnI) cardiac biomarkers in the early and late stages of AMI demand their simultaneous detection, which is difficult to implement using conventional fluorescence and electrochemical technologies. Here, a nanotechnology‐based one‐stop immuno‐surface‐enhanced Raman scattering (SERS) detection platform is reported for multiple cardiac indicators for the rapid screening and progressive tracing of AMI events. Optimal SERS is achieved using optical property‐based, excitation wavelength‐optimized, and high‐yield anisotropic plasmonic gold nanocubes. Optimal immunoassay reaction efficiencies are achieved by increasing immobilized antibodies. Multiple simultaneous detection strategies are implemented by incorporating two different Raman reports with narrow wavenumbers corresponding to two indicators and by establishing a computational SERS mapping process to accurately detect their concentrations, irrespective of multiple enzymes in the human serum. The SERS platform precisely estimated AMI onset and progressive timing in human serum and made rapid AMI identification feasible using a portable Raman spectrometer. This integrated platform is hypothesized to significantly contribute to emergency medicine and forensic science by providing timely treatment and observation.

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Rapid simultaneous SERS detection of dual myocardial biomarkers on single-track finger-pump microfluidic chip

Cited by 19 publications

References 36 publications

Optical Nanobiosensor-Based Point-of-Care Testing for Cardiovascular Disease Biomarkers

Optical Nanobiosensor-Based Point-of-Care Testing for Cardiovascular Disease Biomarkers

Additive manufacturing leveraged microfluidic setup for sample to answer colorimetric detection of pathogens

One‐Stop Plasmonic Nanocube‐Excited SERS Immunoassay Platform of Multiple Cardiac Biomarkers for Rapid Screening and Progressive Tracing of Acute Myocardial Infarction

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