Additive manufacturing leveraged microfluidic setup for sample to answer colorimetric detection of pathogens

Yedire, Sripadh Guptha; Hosseini, Imman Isaac; Shieh, Hamed; Khorrami Jahromi, Arash; AbdelFatah, Tamer; Jalali, Mahsa; Mahshid, Sara

doi:10.1039/d3lc00429e

Cited by 3 publications

(2 citation statements)

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“…, finger-powered, 17 capillary-driven, 18 and simple suction-based systems 19 ) have superiority in terms of being compatible with POC devices. 20,21 These kinds of microfluidics systems have also rapid and mass-producible manufacturing processes in most cases. Additive manufacturing (3D printing) techniques can substantially address the outstanding challenges associated with conventional microfabrication techniques ( e.g.…”

Section: Introductionmentioning

confidence: 99%

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Additively manufactured multiplexed electrochemical device (AMMED) for portable sample-to-answer detection

Khorrami Jahromi,

Siavash Moakhar,

Yedire

et al. 2023

Lab Chip

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Section: Introductionmentioning

confidence: 99%

“…22,23 Indeed, additive manufacturing (AM) can bring about outcomes comparable to traditional methods in terms of temporal and spatial resolution, material, and mechanical properties while being cost-effective, rapid, and scalable. 14,21…”

Section: Introductionmentioning

confidence: 99%

Additively manufactured multiplexed electrochemical device (AMMED) for portable sample-to-answer detection

Khorrami Jahromi,

Siavash Moakhar,

Yedire

et al. 2023

Lab Chip

Self Cite

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AI‐Assisted Plasmonic Enhanced Colorimetric Fluidic Device for Hydrogen Peroxide Detection from Cancer Cells

del Real Mata,

Yedire,

Jalali

et al. 2024

Adv Materials Technologies

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Hydrogen peroxide (H2O2) is an essential molecule to various physiological processes and is commonly used for the detection and monitoring of glucose and cell viability. Furthermore, it is identified as a signal of oncogenic growth due to its widespread presence within the cancer cell environment. However, the low concentrations of H2O2 released by cancer cells' metabolism challenge current detection methods' capabilities and their practicality for translation to clinical applications. Colorimetric assays with simple readouts are a promising solution, provided that their sensitivity and rapidity in detecting H2O2 improve. Here, a plasmonic enhanced nanopatterned platform is proposed coupled with an Amplex Red assay to monitor the color change of H2O2 released from cancer cells. The nanopatterned platform embedded into a multiplexed microfluidic device enhances the kinetics of the reaction ≈7 times. This approach has reached a limit of detection of 1 pm when tested in breast (MCF‐7) and prostate (PC‐3) cancer media. The collected color images are processed and analyzed by a machine learning algorithm that categorizes them into “high” or “low‐to‐no” concentrations of H2O2 with 91% accuracy. This study is a step toward developing a device for highly sensitive H2O2 detection that is easily adaptable, user‐friendly, portable, and automated.

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Fully integrated and automated centrifugal microfluidic chip for point-of-care multiplexed molecular diagnostics

Xiao,

Zhou,

Liu

et al. 2024

Biosensors and Bioelectronics

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Additive manufacturing leveraged microfluidic setup for sample to answer colorimetric detection of pathogens

Abstract: Colorimetric readout for the detection of infectious diseases is gaining traction at the point of care/need owing to its ease of analysis and interpretation, and integration potential with highly specific...

Cited by 3 publications

References 71 publications

Additively manufactured multiplexed electrochemical device (AMMED) for portable sample-to-answer detection

Additively manufactured multiplexed electrochemical device (AMMED) for portable sample-to-answer detection

AI‐Assisted Plasmonic Enhanced Colorimetric Fluidic Device for Hydrogen Peroxide Detection from Cancer Cells

Fully integrated and automated centrifugal microfluidic chip for point-of-care multiplexed molecular diagnostics

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