This study illustrates the novel roles of SodA in serum resistance and biofilm formation in Salm. typhimurium, which may provide a candidate for the biofilm eradication and prevention of infections.
Aims
RpoE is quite immunogenic and can be used as a candidate vaccine for Streptococcus suis infection via immunoproteomics as reported in our previous studies. In this study, we aimed to verify the immunogenicity of recombinant RpoE and its protective effect against of S. suis.
Methods and Results
The RpoE protein was successfully expressed in Escherichia coli, and the purified recombinant protein was mixed with ISA206 to prepare an S. suis subunit vaccine. Mice were immunized with the RpoE subunit vaccine and then infected with the virulent S. suis strain ZY05719. Subunit vaccine‐immunized mice achieved 50% protection, less pathological damage and less bacterial distribution in each organ compared with the control mice. Furthermore, in vitro culture, showed that mouse antisera significantly (P < 0·001) inhibited the growth of S. suis, and qRT‐PCR results showed that RpoE successfully induced the up‐regulation of IL‐6 and TNF‐α cytokines.
Conclusions
RpoE mice were vaccinated to obtain immune protection, which may be candidates for S. suis subunit vaccine.
Significance and Impact of the Study
The results of this study will provide new ideas for the development of safe and effective recombinant subunits vaccines for S. suis.
The effect of glutamine (Gln) on growth performance, intestinal morphology and intestinal barrier function were evaluated in broilers. A total of 320 birds were divided into a control group (CON) and three experimental groups (Gln 1, Gln 2 and Gln 3). Broilers of group CON received basal diet; broilers in group Gln 1, Gln 2 and Gln 3 were supplemented with 0.5%, 1.0% and 1.5 % Gln, respectively, for 42 days. The results indicated that Gln has no influence on the average daily gain (ADG) among the treatments in the periods of 1 to 21 d, 22 to 42 d and 1 to 42 d (P > 0.05). However, Gln improved average daily feed intake (ADFI) and feed intake: average daily gain (F: G), increased the villus height, villus height to crypt depth ratio (V/C) and the activities of sucrose, the ZO1, claudin-1 and occluding mRNA expression levels (P 0.05). Moreover, Gln decreased the crypt depth of jejunum and ileum in broilers at days 21 and 42 (P 0.05). In conclusion, Gln had a positive effect on growth performance and gut parameters by modifying the function of the intestinal mucosa barrier.
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