Functional analysis of superoxide dismutase of<i>Salmonella</i>typhimurium in serum resistance and biofilm formation

Wang, Y.; Li, Yang; Zhang, J.; Sun, Lijun; Wang, Wen; Zhang, C.; Wang, Shizhuang

doi:10.1111/jam.14044

Cited by 12 publications

(13 citation statements)

References 26 publications

(30 reference statements)

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“…Typhimurium LSM52 was isolated from a farm, harbours multiple virulence genes and was highly lethal to mice (Wang et al . ). S .…”

Section: Methodsmentioning

confidence: 97%

“…Bacterial strains and culture conditions S. Typhimurium LSM52 was isolated from a farm, harbours multiple virulence genes and was highly lethal to mice (Wang et al 2018). S. Typhimurium was grown in Luria-Bertani (LB) medium at 37°C.…”

Section: Methodsmentioning

confidence: 99%

“…S. Typhimurium was grown in Luria-Bertani (LB) medium at 37°C. The sodA deletion mutant(LSM52DsodA) and complemented strain (LSM52CDsodA) were constructed in our laboratory (Wang et al 2018). The recombinant eukaryotic expression vectors pcDNA-egfp-LC3B were a gift from Professor Ding (Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences).…”

Section: Methodsmentioning

confidence: 99%

“…Typhimurium functions in resistance to serum and oxidative stress, as well as biofilm formation (Wang et al . ). Several studies have shown that the inactivation of sodA (Mn‐SOD) gene in bacteria is associated with a reduced resistance to ROS resulting in attenuation of virulence in animal models (Poyart et al .…”

Section: Introductionmentioning

confidence: 97%

“…SOD activity, which reduces the levels of superoxide anions, can modulate anti-autophagy in Streptococcus suis (Tang et al 2012). We have recently demonstrated that the superoxide dismutase (SodA) of S. Typhimurium functions in resistance to serum and oxidative stress, as well as biofilm formation (Wang et al 2018). Several studies have shown that the inactivation of sodA (Mn-SOD) gene in bacteria is associated with a reduced resistance to ROS resulting in attenuation of virulence in animal models (Poyart et al 2001;Sansone et al 2002;Pacello et al 2008).…”

Section: Introductionmentioning

confidence: 99%

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Infection with sodA mutant of S. Typhimurium leads to up‐regulation of autophagy in Raw264·7 macrophages

Wang

Liu

Zhang

et al. 2019

Lett Appl Microbiol

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Salmonella enterica serovar Typhimurium (S. Typhimurium) inhabits a wide range of hosts, including poultry, and causes acute gastroenteritis in humans that may result in death. Superoxide dismutase (SOD) is an important antioxidant enzyme present in nearly all living cells exposed to oxygen. Recently, we reported the novel roles of SOD in serum resistance and biofilm formation in S. Typhimurium. This study was designed to explore the effect of infection with sodA mutant of S. Typhimurium on the autophagic response of macrophages. Murine macrophage cell line RAW264·7 was infected with wild‐type (LSM52), a sodA deletion mutant (LSM52ΔsodA) and complemented strain (LSM52CΔsodA). We found that sodA deletion triggered remarkable autophagic responses in infected cells, shown as higher concentrations of LC3‐II or Beclin‐1 than those infected with the wild‐type or complemented strain during the first hour post‐infection in S. Typhimurium. Consistent with these results, the number of viable bacteria in cells infected with the sodA mutant was significantly lower than those infected with wild‐type or complemented strains at 1 h, 2 h and 3 h post‐infection in S. Typhimurium. All results indicated that infection with sodA mutant of S. Typhimurium leads to up‐regulation of autophagy in Raw264·7 macrophages. Significance and Impact of the Study Autophagy plays an important role in Salmonella infection although the role of autophagy in Salmonella infection remains unclear. This study was designed to explore the effect of sodA on the autophagic response of macrophage. We found that infection with sodA mutant of Salmonella Typhimurium could lead to up‐regulation of autophagy in Raw264·7 macrophages.

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“…Typhimurium LSM52 was isolated from a farm, harbours multiple virulence genes and was highly lethal to mice (Wang et al . ). S .…”

Section: Methodsmentioning

confidence: 97%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 97%