Quantitative proteomic analysis of marine biofilms formed by filamentous cyanobacterium

Romeu, Maria J.; Domínguez-Pérez, Dany; Almeida, Daniela; Morais, João; Araújo, Mário Jorge; Osório, Hugo; Campos, Alexandre; Vasconcelos, Vítor; Mergulhão, Filipe

doi:10.1016/j.envres.2021.111566

Cited by 11 publications

(22 citation statements)

References 78 publications

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“…3 ), as already reported for different filamentous cyanobacterial strains 27 , 30 , 43 . In the present study, differences in biofilm development at different shear conditions were noticed in the early stages of biofilm development, but major differences were found in the maturation phase of biofilm development, as previously observed for filamentous 27 , 30 , 43 and coccoid cyanobacteria 25 . Probably, the increased shear rate has a weaker impact during adhesion and initial biofilm formation, but later on, it may promote sloughing and erosion events, affecting biofilm cohesion and structure.…”

Section: Discussionsupporting

confidence: 80%

“…Similar to a previous quantitative study, the majority of identified proteins were related to metabolic processes 30 . Due to optimizations performed in the experimental protocol, the total number of proteins identified in the present study was much higher (1877 vs 546), a 3.5-fold increase compared with our last publication using the FASP + SP3 protocol 30 . These results highly improved the quality of data (e.g., more peptides identified, fewer gaps in the proteomic profile comprising six replicates per condition tested).…”

Section: Discussionsupporting

confidence: 80%

“…Chlorophyll a content monitorization proved to be a useful tool for following cyanobacterial biofilm growth since a correlation between chlorophyll a production and biofilm wet weight was observed (Supplementary Fig. 3 ), as already reported for different filamentous cyanobacterial strains 27 , 30 , 43 . In the present study, differences in biofilm development at different shear conditions were noticed in the early stages of biofilm development, but major differences were found in the maturation phase of biofilm development, as previously observed for filamentous 27 , 30 , 43 and coccoid cyanobacteria 25 .…”

Section: Discussionsupporting

confidence: 75%

“…However, to identify pathways that are triggered under different circumstances to which cells were subjected, quantitative proteomic analyses are the most relevant tool 28 , 29 . A recent quantitative study performed with the unidentified filamentous cyanobacterium LEGE 06007 revealed 41 Differentially Expressed Proteins (DEPs) 30 . The proteomic profile can help in the characterization of virulence factors and cellular processes involved in biofilm regulation.…”

Section: Introductionmentioning

confidence: 99%

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Hydrodynamic conditions affect the proteomic profile of marine biofilms formed by filamentous cyanobacterium

Romeu

Domínguez-Pérez

Almeida

et al. 2022

npj Biofilms Microbiomes

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Proteomic studies on cyanobacterial biofilms can be an effective approach to unravel metabolic pathways involved in biofilm formation and, consequently, obtain more efficient biofouling control strategies. Biofilm development by the filamentous cyanobacterium Toxifilum sp. LEGE 06021 was evaluated on different surfaces, glass and perspex, and at two significant shear rates for marine environments (4 s−1 and 40 s−1). Higher biofilm development was observed at 4 s−1. Overall, about 1877 proteins were identified, and differences in proteome were more noticeable between hydrodynamic conditions than those found between surfaces. Twenty Differentially Expressed Proteins (DEPs) were found between 4 s−1 vs. 40 s−1. On glass, some of these DEPs include phage tail proteins, a carotenoid protein, cyanophynase glutathione-dependent formaldehyde dehydrogenase, and the MoaD/ThiS family protein, while on perspex, DEPs include transketolase, dihydroxy-acid dehydratase, iron ABC transporter substrate-binding protein and protein NusG. This study contributes to developing a standardized protocol for proteomic analysis of filamentous cyanobacterial biofilms. This kind of proteomic analysis can also be useful for different research fields, given the broad spectrum of promising secondary metabolites and added-value compounds produced by cyanobacteria, as well as for the development of new antibiofilm strategies.

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Section: Discussionsupporting

confidence: 80%

Section: Discussionsupporting

confidence: 80%

Section: Discussionsupporting

confidence: 75%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Hydrodynamic conditions affect the proteomic profile of marine biofilms formed by filamentous cyanobacterium

Romeu

Domínguez-Pérez

Almeida

et al. 2022

npj Biofilms Microbiomes

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“…Moreover, one of the key issues in the development of new AF surfaces is the necessary screening of candidate surfaces (usually tested in vitro in a first step prior to in situ testing) before scale-up and final performance evaluation. Since it has been shown that hydrodynamics can severely affect biofilm formation [ 87 , 88 , 89 , 90 ] and gene expression by fouling organisms [ 91 , 92 ], it is recommended that these tests be performed in controlled hydrodynamic conditions that mimic the final application scenario [ 93 ].…”

Section: Resultsmentioning

confidence: 99%

Antifouling Performance of Carbon-Based Coatings for Marine Applications: A Systematic Review

2022

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Although carbon materials are widely used in surface engineering, particularly graphene (GP) and carbon nanotubes (CNTs), the application of these nanocomposites for the development of antibiofilm marine surfaces is still poorly documented. The aim of this study was, thus, to gather and discuss the relevant literature concerning the antifouling performance of carbon-based coatings against marine micro- and macrofoulers. For this purpose, a PRISMA-oriented systematic review was conducted based on predefined criteria, which resulted in the selection of thirty studies for a qualitative synthesis. In addition, the retrieved publications were subjected to a quality assessment process based on an adapted Methodological Index for Non-Randomized Studies (MINORS) scale. In general, this review demonstrated the promising antifouling performance of these carbon nanomaterials in marine environments. Further, results from the revised studies suggested that functionalized GP- and CNTs-based marine coatings exhibited improved antifouling performance compared to these materials in pristine forms. Thanks to their high self-cleaning and enhanced antimicrobial properties, as well as durability, these functionalized composites showed outstanding results in protecting submerged surfaces from the settlement of fouling organisms in marine settings. Overall, these findings can pave the way for the development of new carbon-engineered surfaces capable of preventing marine biofouling.

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Understanding the flow behavior around marine biofilms

Romeu,

Miranda,

de Jong

et al. 2024

Biofilm

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Quantitative proteomic analysis of marine biofilms formed by filamentous cyanobacterium

Cited by 11 publications

References 78 publications

Hydrodynamic conditions affect the proteomic profile of marine biofilms formed by filamentous cyanobacterium

Hydrodynamic conditions affect the proteomic profile of marine biofilms formed by filamentous cyanobacterium

Antifouling Performance of Carbon-Based Coatings for Marine Applications: A Systematic Review

Understanding the flow behavior around marine biofilms

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