Immunogenicity and protective ability of RpoE against
            <i>Streptococcus suis</i>
            serotype 2

Li, Yang; Du, Yansheng; Mao, Chenlong; Li, J.; Jin, Muchun; Sun, Lijun; Wang, Y.

doi:10.1111/jam.14874

Cited by 8 publications

(14 citation statements)

References 34 publications

(39 reference statements)

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“…Since then, neutrophils in the lesions were characterized in various swine studies [9,129,130]. In mouse models, neutrophils also infiltrate both systemic [101,[131][132][133][134][135][136] and brain lesions [7,137].…”

Section: S Suis-induced Lesions Contain High Infiltration Of Neutrophilsmentioning

confidence: 99%

“…The presence of neutrophils in the lesions allows scoring the importance of the disease induced by S. suis since the accumulation of neutrophils witnesses a severe inflammation. Thus, neutrophil infiltration was investigated to measure the protection against S. suis in immunization trials in pigs and mice [39,131,135,137,138]; to compare the pathological differences between serotype 2 and 9 in infected pigs [41]; to clarify the importance of virulence factors in the bacterial pathogenesis [89,132]; and to evaluate the severity of the disease caused by the coinfection of S. suis and the porcine reproductive and respiratory syndrome virus [139].…”

Section: S Suis-induced Lesions Contain High Infiltration Of Neutrophilsmentioning

confidence: 99%

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Neutrophils in Streptococcus suis Infection: From Host Defense to Pathology

2021

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Streptococcus suis is a swine pathogen and zoonotic agent responsible for economic losses to the porcine industry. Infected animals may develop meningitis, arthritis, endocarditis, sepsis and/or sudden death. The pathogenesis of the infection implies that bacteria breach mucosal host barriers and reach the bloodstream, where they escape immune-surveillance mechanisms and spread throughout the organism. The clinical manifestations are mainly the consequence of an exacerbated inflammation, defined by an exaggerated production of cytokines and recruitment of immune cells. Among them, neutrophils arrive first in contact with the pathogens to combat the infection. Neutrophils initiate and maintain inflammation, by producing cytokines and deploying their arsenal of antimicrobial mechanisms. Furthermore, neutrophilic leukocytosis characterizes S. suis infection, and lesions of infected subjects contain a large number of neutrophils. Therefore, this cell type may play a role in host defense and/or in the exacerbated inflammation. Nevertheless, a limited number of studies addressed the role or functions of neutrophils in the context of S. suis infection. In this review, we will explore the literature about S. suis and neutrophils, from their interaction at a cellular level, to the roles and behaviors of neutrophils in the infected host in vivo.

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Section: S Suis-induced Lesions Contain High Infiltration Of Neutrophilsmentioning

confidence: 99%

Section: S Suis-induced Lesions Contain High Infiltration Of Neutrophilsmentioning

confidence: 99%

Neutrophils in Streptococcus suis Infection: From Host Defense to Pathology

2021

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“…Several antigenic proteins of S. suis have been tested as subunit vaccine candidates, but S. suis vaccine development is still ongoing [ 2 , 9 ]. Compared to intracellular proteins, extracellular and surface proteins of bacterial pathogens have the advantage of being easily recognized by the infected host and thus are candidates for vaccine development [ 10 , 30 ].…”

Section: Discussionmentioning

confidence: 99%

“…Therefore, it is necessary to develop an efficient vaccine to protect both pigs and humans from S. suis infection. Subunit vaccines based on proteins conserved among serotypes provide a strong cross-immunity, which may be more useful in the field if they protect against challenge with strains of heterologous serotypes [ 8 , 9 , 10 ]. In this study, three S. suis virulence-associated proteins—muramidase-released protein (MRP), glyceraldehyde-3-phosphate dehydrogenase (GAPDH), and dihydrolipoamide dehydrogenase (DLD), which are highly conserved in SS2, SS7, and SS9—were considered subunit vaccine candidates [ 11 , 12 ].…”

Section: Introductionmentioning

confidence: 99%

TLR4 Agonist Combined with Trivalent Protein JointS of Streptococcus suis Provides Immunological Protection in Animals

et al. 2021

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Streptococcus suis (S. suis) serotype 2 (SS2) is the causative agent of swine streptococcosis and can cause severe diseases in both pigs and humans. Although the traditional inactive vaccine can protect pigs from SS2 infection, novel vaccine candidates are needed to overcome its shortcomings. Three infection-associated proteins in S. suis—muramidase-released protein (MRP), glyceraldehyde-3-phosphate dehydrogenase (GAPDH), and DLD, a novel putative dihydrolipoamide dehydrogenase—have been previously identified by immunoproteomic assays. In this study, the effective immune protection of the recombinant trivalent protein GAPDH-MRP-DLD (JointS) against SS2, SS7, and SS9 was determined in zebrafish. To improve the immune efficacy of JointS, monophosphoryl lipid A (MPLA) as a TLR4 agonist adjuvant, which induces a strong innate immune response in the immune cells of mice and pigs, was combined with JointS to immunize the mice. The results showed that immunized mice could induce the production of a high titer of anti-S. suis antibodies; as a result, 100% of mice survived after SS2 infection. Furthermore, JointS provides good protection against virulent SS2 strain infections in piglets. Given the above, there is potential to develop JointS as a novel subunit vaccine for piglets to prevent infection by SS2 and other S. suis serotypes.

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“…Th1-type and Th2-type immune responses are critical for resistance to S. suis infection [9]. Sera, lung, and spleen samples of immunized mice at 10 days post-booster immunization were collected, and ELISA was used to analyze these samples for the induction level of IFN-γ and IL-4, as indicator cytokines for Th1-type and Th2-type immune responses in S. suis infection, respectively [23][24][25][26][27]. For sera samples, compared with the PBS control group, the immunization of mice with rPstB or rPstB-epitope generated significantly higher levels of both IFN-γ and IL-4.…”

Section: Protection Of Rpstb and Rpstb-epitope Against S Suis Infecti...mentioning

confidence: 99%

Subunit Vaccine Targeting Phosphate ABC Transporter ATP-Binding Protein, PstB, Provides Cross-Protection against Streptococcus suis Serotype 2, 7, and 9 in Mice

Yan

Yao

Pan

et al. 2023

Veterinary Sciences

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Streptococcus suis is a significant pathogen in pigs and a newly emerging zoonotic agent in humans. The presence of multiple serotypes and strains with diversified sequence types in pig herds highlights the need for the identification of broadly cross-reactive universal vaccine antigen targets, capable of providing cross-protection against S. suis infection. Subunit vaccines based on the conserved proteins shared between different S. suis serotypes are potential candidates for such a universally protective vaccine. In the present study, phosphate ABC transporter ATP-binding protein PstB (PstB), an immunogenic protein of the S. suis bacterium, was expressed and purified, and then subjected to cross-protection evaluation in mice. The PstB protein showed nearly 100% amino acid similarity across a panel of 31 S. suis isolates representing different serotypes, which were collected from different countries. A recombinant PstB (rPstB) protein (S. suis serotype 2) was recognized by rabbit sera specific to this serotype, and induced high levels of IFN-γ and IL-4 in mice immunized with the recombinant protein. These cytokines are considered important for protection against S. suis infection. Immunization of mice with rPstB resulted in an 87.5% protection against challenge with S. suis serotype 2 and 9 strains, suggesting a high level of cross-protection for S. suis serotypes 2 and 9. A lower protection rate (62.5%) was observed in mice challenged with the S. suis serotype 7 strain. These data demonstrate that PstB is a promising target antigen for development as a component of a universal subunit vaccine against multiple S. suis serotypes.

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Immunogenicity and protective ability of RpoE against Streptococcus suis serotype 2

Cited by 8 publications

References 34 publications

Neutrophils in Streptococcus suis Infection: From Host Defense to Pathology

Neutrophils in Streptococcus suis Infection: From Host Defense to Pathology

TLR4 Agonist Combined with Trivalent Protein JointS of Streptococcus suis Provides Immunological Protection in Animals

Subunit Vaccine Targeting Phosphate ABC Transporter ATP-Binding Protein, PstB, Provides Cross-Protection against Streptococcus suis Serotype 2, 7, and 9 in Mice

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