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The aim of this study was to investigate the effects of glutamine (Gln) on the intestinal mucosal structure and immune cells of broilers infected with Salmonella Enteritidis. 160 1-d-old commercial Arbor Acres (AA) broilers were randomly selected to receive one of four treatments, each of which had 5 replicates. Each replicate consisted of 8 chicks subjected to a 21-d feeding trial. Group I served as the unchallenged (CON). All birds in groups II (SCC) -IV were challenged with 2.0 × 10 4 CFU/mL of S. Enteritidis. The birds in groups III and IV were treated with 0.5% and 1.0% Gln. The results showed that S. Enteritidis infection led to a decrease in the relative length and weight, villus height:crypt depth (VH:CD) of the jejunum and ileum, the number of intraepithelial lymphocyte cells, and number of goblet cells and an increase in the number of mast goblet cells compared with the measurements of these parameters in the CON group (p<0.05). In addition, the Gln groups had increased relative length and weight, VH:CD of the jejunum and ileum, numbers of intraepithelial lymphocyte cells, and numbers of goblet cells and decreased crypt depth in the jejunum and ileum and numbers of mast goblet cells compared with the measurements of these parameters in the SCC group (p<0.05). It was concluded that Gln added to broiler diets can effectively alleviate the intestinal mucosal damage caused by S. Enteritidis infection and improve its normal defense barrier function.

The effect of glutamine (Gln) on growth performance, intestinal morphology and intestinal barrier function were evaluated in broilers. A total of 320 birds were divided into a control group (CON) and three experimental groups (Gln 1, Gln 2 and Gln 3). Broilers of group CON received basal diet; broilers in group Gln 1, Gln 2 and Gln 3 were supplemented with 0.5%, 1.0% and 1.5 % Gln, respectively, for 42 days. The results indicated that Gln has no influence on the average daily gain (ADG) among the treatments in the periods of 1 to 21 d, 22 to 42 d and 1 to 42 d (P > 0.05). However, Gln improved average daily feed intake (ADFI) and feed intake: average daily gain (F: G), increased the villus height, villus height to crypt depth ratio (V/C) and the activities of sucrose, the ZO1, claudin-1 and occluding mRNA expression levels (P 0.05). Moreover, Gln decreased the crypt depth of jejunum and ileum in broilers at days 21 and 42 (P 0.05). In conclusion, Gln had a positive effect on growth performance and gut parameters by modifying the function of the intestinal mucosa barrier.

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This study was conducted to investigate the effects of glutamine (Gln) on blood biochemistry and activity of serum enzymes in broilers challenged with Salmonella enteritidis. 240 one-day-old Arbor Acres broilers were distributed into four groups treated in a completely randomized design for 21 days. The groups included a noninfection control (CON); infected with S. enteritidis (SCC); infected +0.5% Gln (Gln 1), and infected +1.0% Gln (Gln 2). The plasma total protein, albumin, and globulin concentration, AST, LDH and CK activity in SCC group were lower than the CON overall except the albumin at 14, 21 d, and CK at 14 d. SCC group had lower RBC at 7 d; lower MCHC at 14 d; higher MCHC, and MCH at 7 d, or 21 d; higher WBC at d 7, 14, and 21; higher γ-GGT activity at d 14, and 21than the CON group. Gln group had higher plasma total protein, albumin, and globulin concentration, AST, LDH and CK activity than those in SCC overall except the albumin at 14, 21 d, and CK at 14 d. Gln group had higher RBC at 7 d; higher MCHC at 14 d; lower MCHC, and MCH at 7 d, or 21 d; lower WBC at d 7, 14, and 21, and lower γ-GGT activity at d 14, and 21 than the SCC group. These results suggest that Gln could effective improve the physiological responses and maintain the normal health status of S. enteritidis infection of broilers.