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The antioxidant and anti-adipogenic activities of a mixture of Nelumbo nucifera L., Morus alba L., and Raphanus sativus were investigated and their anti-obesity activities were established in vitro and in vivo. Among the 26 different mixtures of extraction solvent and mixture ratios, ethanol extract mixture no. 1 (EM01) showed the highest antioxidant (α,α-Diphenyl-β-picrylhydrazyl, total phenolic contents) and anti-adipogenic (Oil-Red O staining) activities. EM01 inhibited lipid accumulation in 3T3-L1 adipocytes compared to quercetin-3-O-glucuronide. Furthermore, body, liver, and adipose tissue weights decreased in the high-fat diet (HFD)-EM01 group compared to in the high-fat diet control group (HFD-CTL). EM01 lowered blood glucose levels elevated by the HFD. Lipid profiles were improved following EM01 treatment. Serum adiponectin significantly increased, while leptin, insulin growth factor-1, non-esterified fatty acid, and glucose significantly decreased in the HFD-EM01 group. Adipogenesis and lipogenesis-related genes were suppressed, while fat oxidation-related genes increased following EM01 administration. Thus, EM01 may be a natural anti-obesity agent.

Antioxidants are used to prevent the oxidation of foods. When used for food additive purposes, the dosage should be regulated and the functionality evaluated to ensure stability. In this study, we performed a method validation for the quantitative analysis of rosemary extract residues and evaluated the antioxidant activity of rosemary extract in food matrices. The validated method was able to determine rosemary extract under the optimized high-performance liquid chromatography-photodiode array (HPLC-PDA) conditions. Furthermore, the antioxidant activity was evaluated by peroxide value, acid value, and in terms of the residual antioxidant levels in lard oil. For HPLC-PDA analysis, the limit of detection and quantification of rosemary extracts was ranged from 0.22 to 1.73 μg/mL, 0.66 to 5.23 μg/mL and the recoveries of the rosemary extracts ranged from 70.6 to 114.0%, with relative standard deviations of between 0.2% and 3.8%. In terms of antioxidant activity, carnosic acid performed better than carnosol. Furthermore, by evaluation of the residual antioxidant level using HPLC, we found that carnosic acid is more stable in lard oil than carnosol. These results indicate that rosemary extract can be used as an antioxidant and that the analytical method is suitable for the determination of rosemary extract in various food samples.

Cucurbita moschata is widely planted in most parts of the world, and is rich in carotenoids, vitamins, dietary fiber, minerals, and phenolic compounds. It also has important medicinal value. Some related research has proven that Cucurbita moschata has the potential ability to induce anti-obesity, anti-diabetic, antibacterial, and anticancer effects. At the same time, it has attracted more attention in the medical field. These nutrients and bioactive compounds in Cucurbita moschata have important effects on human health. In order to make better use of this crop, it still needs further study. Therefore, the purpose of this article is to summarize the physicochemical properties and nutritional components of Cucurbita moschata, and to provide a reference for further research on the benefits of on human health.

Korean red pine (Pinus densiflora) belongs to the Genus Pinus, and its bark contains a great amount of naturally occurring phenolic compounds. Until now, few studies have been conducted to assess the neuroprotective effects of Pinus densiflora bark extract against brain ischemic injury. The aim of this study was to investigate the neuroprotective effects of pre-treatment with the extract in the hippocampus following 5-min transient forebrain ischemia in gerbils. Furthermore, this study examined the anti-inflammatory effect as a neuroprotective mechanism of the extract. Pinus densiflora bark was extracted by pure water (100 °C), and this extract was quantitatively analyzed and contained abundant polyphenols, flavonoids, and proanthocyanidins. The extract (25, 50, and 100 mg/kg) was orally administered once a day for seven days before the ischemia. In the gerbil hippocampus, death of the pyramidal neurons was found in the subfield cornu ammonis 1 (CA1) five days after the ischemia. This death was significantly attenuated by pre-treatment with 100 mg/kg, not 25 or 50 mg/kg, of the extract. The treatment with 100 mg/kg of the extract markedly inhibited the activation of microglia (microgliosis) and significantly decreased the expression of pro-inflammatory cytokines (interleukin 1β and tumor necrosis factor α). In addition, the treatment significantly increased anti-inflammatory cytokines (interleukin 4 and interleukin 13). Taken together, this study clearly indicates that pre-treatment with 100 mg/kg of Pinus densiflora bark extract in gerbils can exert neuroprotection against brain ischemic injury by the attenuation of neuroinflammatory responses.

PPP-RTK which takes full advantages of both Real-Time Kinematic (RTK) and Precise Point Positioning (PPP), is able to provide centimeter-level positioning accuracy with rapid integer Ambiguity Resolution (AR). In recent years, with the development of BeiDou Navigation Satellite System (BDS) and Galileo navigation satellite system (Galileo) as well as the modernization of Global Positioning System (GPS) and GLObal NAvigation Satellite System (GLONASS), more than 140 Global Navigation Satellite System (GNSS) satellites are available. Particularly, the new-generation GNSS satellites are capable of transmitting signals on three or more frequencies. Multi-GNSS and multi-frequency observations become available and can be used to enhance the performance of PPP-RTK. In this contribution, we develop a multi-GNSS and multi-frequency PPP-RTK model, which uses all the available GNSS observations, and comprehensively evaluate its performance in urban environments from the perspectives of positioning accuracy, convergence and fixing percentage. In this method, the precise atmospheric corrections are derived from the multi-frequency and multi-GNSS observations of a regional network, and then disseminated to users to achieve PPP rapid AR. Furthermore, a cascade ambiguity fixing strategy using Extra‐Wide‐Lane (EWL), Wide-Lane (WL) and L1 ambiguities is employed to improve the performance of ambiguity fixing in the urban environments. Vehicle experiments in different scenarios such as suburbs, overpasses, and tunnels are conducted to validate the proposed method. In suburbs, an accuracy of within 2 cm in the horizontal direction and 4 cm in the vertical direction, with the fixing percentage of 93.7% can be achieved. Compared to the GPS-only solution, the positioning accuracy is improved by 87.6%. In urban environments where signals are interrupted frequently, a fast ambiguity re-fixing can be achieved within 5 s. Moreover, multi-frequency GNSS signals can further improve the positioning performance of PPP-RTK, particularly in the case of small amount of observations. These results demonstrate that the multi-frequency and multi-GNSS PPP-RTK is a promising tool for supporting precise vehicle navigation.

Integrity relates to the trust that can be placed in the correctness of information supplied by a navigation system. It includes the ability of the navigation system to provide timely warnings to users when the system fails to meet its stated accuracy. Specifically, a navigation system is required to deliver a warning (alarm) when the error in the derived user position solution exceeds an allowable level (alarm limit). This warning must be issued to the user within a given period of time (time-to-alarm) and with a given probability (integrity risk). The two main approaches to monitoring the integrity of satellite navigation systems are Receiver Autonomous Integrity Monitoring (RAIM), and monitoring based on an independent network of integrity monitoring stations and a dedicated Ground Integrity Channel (GIC). More recently Satellite Autonomous Integrity Monitoring (SAIM) methods have also been investigated. This paper presents the results of a study to assess the RAIM capability of the Galileo system when used alone and when combined with the Global Positioning System (GPS). The assessment was based on the Marginally Detectable Error (MDE) algorithm. The results show a significant improvement in the capability to perform RAIM using a combined Galileo/GPS system compared to the performance using the Galileo system alone. This study was supported by Alcatel Space and was a contribution to the Galileo definition studies carried out for the European Community under the GALA project.

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