Cyanobacteria have been known as a potential extracellular-polysaccharide (EPS) producer. The objective of this study was to screen the marine cyanobacteria as potential antidiabetic agents. The present investigation was designed to determine the antidiabetic activity of EPS, intracellular-polysaccharide (IPS) and biomass extracts from marine cyanobacteria isolates. 10 cyanobacteria isolates were cultivated in IMK medium, at 25 o C for 21 days. The morphology of cells was identified by a light microscope. EPS and IPS were separated by ethanol precipitation method and their antidiabetic activity was analyzed by the inhibition of α-glucosidase activity method. Results of morphology identification of 10 cyanobacteria isolates consist of Oscillatoria limnetica, Oscillatoria sp., Leptolyngbya sp., Pseudanabaena sp., Lyngbya sp. and Phormidium sp., Coelastrella sp., Aphanothece sp. and Synechococcus sp., and Chroococcus sp. Almost all of EPS from marine cyanobacteria isolates were potential as inhibitor of α-glucosidase, except for Oscillatoria limnetica and Phormidium sp. isolates. The highest activity in α-glucosidase inhibition was detected in Pseudanabaena sp. (14.02%) and Chroococcus sp. (13.0%) isolates.

No abstract

Priatni S, Ratnaningrum D, Kosasih W, Sriendah E, Srikandace Y, Rosmalina T, Pudjiraharti S. 2018. Protein and fatty acidprofile of marine fishes from Java Sea, Indonesia. Biodiversitas 19: 1737-1742. Indonesia is the second largest producer of capturefisheries products in the world and the most capture fisheries production comes from marine fisheries. Marine fish is a source of protein,amino acid, saturated and unsaturated fatty acids, which are important components of diet. The objective of the study was to investigatethe protein and fatty acids profile of nine marine fish samples from Java Sea of Indramayu West Java, Indonesia. The analysis datashowed that the total protein content of fish samples ranged from 61.07% (Pampus argenteus) to 86.56% (Tetraodontidae). Meanwhile,total lipid content of fish samples ranged from 1.73% (Tetraodontidae) to 9.82% (Leiognathus equulus). The concentration of α-AminoNitrogen (AN) of fish protein hydrolysate was ranging from 31 mM (Nemipterus hexodon) to 69 mM (Mystacoleucus padangensis)and% Degree of Hydrolysis (DH) was ranging from 9.33% to 20.39%. The molecular weight of protein fish samples had similar profilesprimarily for almost all samples, which could be observed from a typical band with the weight around 49 kDa. The saturated fatty acid(Ʃ SFA) compositions of fish species ranged from 1094.03-4233.03 μg/g. Oleic acid (MUFA) content of all fish species ranged from257.91-1216.06 μg/g. However, only three fish species contain of Poly Unsaturated Fatty Acid (PUFA) linoleic acid as the following;Selaroides leptolepis (171.36 μg/g), Oxyeleotris marmorata (249.40μg/g) and Tetraodontidae (140.35 μg/g). The highest SFA contentwas found in S. leptolepis with palmitic acid (C16:0) as the dominant saturated fatty acid (2320.88 μg/g). S. leptolepis also containedhigh oleic acid (1216.06 μg/g) and linoleic acid (171.36 μg/g).

No abstract

No abstract

Production of omega-3 fatty acids from lemuru fish by-products was studied by enzymatic hydrolysis using a lipase enzyme in one liter of the batch reactor. The hydrolysis temperature of fish oil was set at 45 to 55 ℃ for 0 to 24 h, whereas agitation from 50 to 150 rpm. RSM-Box Bhenken was used to study the effect of these parameters on omega-3 (EPA, docosahexaenoic acid (DHA), and α-linolenic acid (ALA)) content. The % free fatty acid (FFA), acid index, peroxide index, iodine index, and saponification index of lemuru fish oil was 0.925, 2.52, 42.5, 97.28, and 160.11%, respectively. GC-MS analysis results showed that unsaturated fatty acids content (62.34%), which are consisted of omega-3 (EPA, DHA, and ALA), omega-6 and omega-9, was much higher than saturated acids (12.97%). The experiment data showed that the highest EPA (1.221%) and DHA (0.312%) content were reached at 50 ℃ and 24 h with 150 rpm of agitation. However, through the RSM-Box Bhenken analysis and 3D surface plot, it was suggested that the optimum condition was obtained at 45 ℃ and 24 h with 150 rpm of agitation with the content of EPA, DHA, and ALA were 1.709, 0.49, and 1.237%, respectively.

No abstract

Microalgae are rich source of primary and secondary metabolites which have potential bioactive compounds for application in the pharmaceutical industry. The study of antidiabetic activity from microalgae have been carried out because it can be cultivated in a small area and efficient in light capturing. This study aimed to screen the local marine microalgae which potential as the antidiabetic source. Five microalgae strains (Chlorella sp A and B, Nanochlropsis sp, Porphyridium sp, and Skeletonema sp were screened according to their growth rates, antidiabetic activity (α-glucosidase inhibition) and biomass yield. Results showed that Porphyridium sp cells were grown very active compared to other strains. After 8 days of cultivation, the cells’ number of Porphyridium sp was 345x103 cells/ml. The highest activity in α-glucosidase inhibition was detected in Porphyridium sp biomass (12.63%). The biomass yield of Porphyridium sp (6.71 g) was obtained after cultivated at 5 L scale production. The exponential growth rate of Porphyridium sp which cultivated with aeration pump system (Rexp = 0.177, TD = 3.8 days) was higher than cultivated with shaker incubator system (Rexp = 0.045, TD = 15.4 days). We concluded that Porphyridium sp is the best microalgae compared to other microalgae both its productivity and antidiabetic activity. The cultivation of Porphyridium sp by using aeration pump system is much recommended.