Paku atai plant (Angiopteris ferox Copel), specifically the tuber has been used empirically
as an anticancer and antidote materials by Dayaknese in West Kutai, with limited
scientific study. Thus, this research had been conducted to determine and evaluate the total
phenolic, flavonoid content of this plant along with the antioxidant and toxicity profile.
The research used several extracts’ solvents including extraction with ethanol (crude
extract, CE) and subsequently partitioned with n-hexane (HF); ethyl acetate (EF) and
ethanol aqueous (EAF). The resulted crude extract and fractions were then analyzed
through colorimetric method to determine the phenolic and flavonoid total; with DPPH
and FRAP to observe the antioxidant activity; and using BSLT method to evaluate the
toxicity activity. The results showed that the EF fraction provided the strongest antioxidant
activity with IC50 value of 13.79 μg/mL and iron reduction with FRAP value of
387.5±6.41 µM/g. However, a high correlation was observed between the antioxidant and
the total phenolic content (r2
= 0.970 - 0.974) but little correlation in total flavonoid (r2
=
0.345 - 0.373). Furthermore, the EF (19.56±7.35) showed the highest toxicity activity
followed by CE (22.42±2.10), HF (39.52±7.38), and EAF (41.75±5.10). Therefore, the
paku atai tuber can be potentially developed as a natural antioxidant and anticancer
material.
Bligo fruit (Benincasa hispida Thunb) is one of medicinal plants that used to cure typhoid fever which caused by Salmonella typhi bacteria. The aim of this research is to find ont the effect of ethanol concentration as a solvent from Bligo fruit (Benincasa hispida Thunb) extracts as an antibacterial, especially to Salmonella typhi. This simplicia was being extracted with 96% ethanol, 70% ethanol, and 50% ethanol by using maceration method for 3x24 hours. The antibacterial activity test was done by diffusion of agar method to the MHA medium by obserring the resistor zone which formed from 96% ethanol, 70% ethanol and 50% ethanol. The diametres that have been obtained are 6,815 mm for 96% ethanol, 25,223 mm for 70% ethanol while for 50% ethanol there is no resisten zone can be seen. From the result canbe conclude that the best concentration of ethanol that has a great potencial as resistor to Salmonella typhi bacteria is the Bligo fruit (Benincasa hispida Thunb) extracts of 70% ethanol.Key words: Bligo fruit (Benincasa hispida Thunb), Antibacterial, Salmonella typhi.
The increasing number of deaths caused by infection encourage researchers conduct early stage in the search for new antibiotics to resolve the issue. This study aims to isolate microbes aktinomicetes contained in karst areas of Bantimurungtourist park district South Sulawesi province to see the antimicrobial inhibitory activity. The initial stage to get new antibiotics, researcher isolated, identified, characterized and tested the antimicrobial activity of isolates to be studied. One of microbes widely studied to be taken of its active compound is aktinomycetes. The research phase is actinomycetes isolation, isolates purification, and testing isolates antagonistic actinomycetes to see whether there is a power resistor formed. From the results obtained 8 isolates actinomycetes isolation, but only 6 isolates that can be grown back properly. Six of isolates actinomycetes obtained, tested antagonist was done to see the activity of inhibitory power. Two of active isolateswas obtained that have inhibitory against several microbes that P. aeruginosa and S. aureus which is then coded isolates of K21 and K32.
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