Abstract.A research about Citrus medica L. var sarcodactylis had been conducted and it showed a significant antimicrobial activity, thus drive our curiosity to investigate the other variety from the same species, Citrus medica L. var proper. This research focuses in chemical compound study and antimicrobial activity screening against Staphylococcus aureus, Propionibacterium acne, and Candida albicans of Citrus medica L. var Proper leaves' essential oil. The essential oil is distillated from fresh leaves by hydrodestillation. The chemical compound was analysed using GC-MS instrument while the antimicrobial activity was tested using disk diffusion method. The results showed that the major component of the essential oil was Z-citral, citral and limonene compounds. The antimicrobial activity test results against the test microorganism are 9.15±0.15 mm, 11.15±1.3 mm and 8.02±0.48 mm, consecutively, for Staphylococcus aureus, Propionibacterium acne and Candida albicans.
Breadfruit plant has been empirically used by the society as antidiabetic. The aim of this study is to understand the α-glucosidase enzyme inhibitory activity of bread fruit leaves (Artocarpus altilis) and their phytochemical profile. The varieties of leaf used were the yellow and green breadfruit leaves. Each was extracted with multiple maceration using n-hexane and 70% ethanol as solvents. From the phytochemistry identification, it was found that the ethanol extract of green breadfruit leaf contains alkaloid, flavonoid, triterpene/steroid, polyphenol, while the yellow breadfruit leaf contains flavonoid and triterpene/steroid. The result of α-glucosidase inhibitory activity test shows that ethanol extracts of both yellow and green breadfruit leaf (Artocarpus altilis (Parkinson) Forberg) have the greatest enzyme inhibitory activity with IC50 of 9.07 and 11.01 ppm, respectively, compared to the n-hexane extracts of both yellow and green breadfruit leaf with IC50 of 16.16 and 23.24 ppm, respectively. Meanwhile, the positive control, acarbose has the strongest inhibitory activity with IC50 of 6.79 ppm.
Abstract. Rante H, Alam G, Usmar, Zahra S, Kurniawati A, Ali A. 2022. Antimicrobial activity of Streptomyces spp. sponge-associated isolated from Samalona Island of South Sulawesi, Indonesia. Biodiversitas 23: 1392-1398. The resistance case of infectious diseases increases annually, while the necessity of antibiotics as a drug choice for therapy most important is needed. One of the efforts to overcome these problems is an investigation of new antibiotic sources, namely marine organisms. Actinomycetes are microbes well known as more antibiotic-producing than fungi and bacteria. This study aims to the identification of marine actinomycetes isolated from sponge samples in Samalona Island, South Sulawesi, Indonesia. The antimicrobial activity was evaluated against multi-drug resistant (MDR) Staphylococcus aureus, Escherichia coli, and Candida albicans. Isolation was carried out using SCA (Starch Casein Agar) medium by the pour plate method. The selected isolates were designated as SML10h and SML10a. The isolates SML 10h and SML 10a were recovered from sponges Haliclona sp. and Aplysina sp., respectively. The production of antimicrobial substances was performed by fermentation of isolates in SNB (Starch Nitrate Broth) medium for 14 days. The antimicrobial activity was shown by the ethyl acetate extract with 2 mg/20 ?L concentration against all test microbes. Phylogenetic analysis of the 16S rRNA gene sequences revealed that the similarity of SML 10a was most closely related to Streptomyces geysiriensis (98%).
Background:
Endophytic fungi live in plants’ tissue and can produce the same bioactive
compounds as its host plant produces. Syzygiumpolyanthum leaves have known to be one of
the antibacterial compound producers.
Aim and Objective:
This study aimed to characterize morphologically, microscopically, and
molecularly the antibacterial-producing endophytic fungi of Syzygiumpolyanthum leaves.
Methods:
The isolation of endophytic fungi was done by fragment planting method on PDA medium.
The antibacterial screening was performed using the antagonistic test as the first screening
followed by the disc diffusion test method. The morphological characterization was based on isolate’s
mycelia color, growth pattern, margin, and surface texture of the colony, while the microscopic
characterization was based on its hyphae characteristics. The molecular characterization of
the isolate was done by nitrogen base sequence analysis method on nucleotide constituent of ITS
rDNA genes of the isolate.
Results:
The results found that isolate DF1 has antibacterial activity against E.coli, S.aureus,
P.acne, and P.aeruginosa, with the greatest inhibition at 10% concentration of broth fermentation
extract on S.aureus with a diameter of inhibition of 13.77 mm.
Conclusion:
Based on macroscopic, microscopic, and molecular characterization, DF1 isolate is
similar to Ceriporialacerate.
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