Aim:This research aimed to identify Staphylococcus species isolated from preputial swabs of healthy Aceh cattle, based on 16S ribosomal RNA gene analysis.Materials and Methods:The bacterium was isolated from preputial swabs of healthy Aceh cattle. The total DNA from the isolated bacteria was extracted using the Genomic DNA Mini Kit followed by polymerase chain reaction (PCR) amplification of the 16S rRNA gene. The product of PCR amplification was then sequenced and aligned to the known sequences in the GenBank database by multiple alignments and was also analyzed by bioinformatics software to construct a phylogenetic tree.Results:The results revealed that the bacterial isolate 3A had genetically closed relation to Staphylococcus pasteuri with <97% maximum identity. Data derived from the phylogenetic tree revealed that the bacterial isolate 3A was also related to Staphylococcus warneri, yet, it shows a different evolutionary distance with the ancestors (S. pasteuri).Conclusion:The results of this research suggested that the bacterium 3A, isolated from preputial swabs of healthy Aceh cattle, is a Staphylococcus species.

Tuna madidihang (Thunnus albacares) merupakan jenis tuna yang memiliki nilai ekonomis penting, dan termasuk ke dalam kelompok jenis pelagis besar, dengan siklus hidupnya berada di perairan tropis dan sub-tropis. Madidihang beruaya pada lintas perairan untuk mencari sumber makanan dan melakukan pemijahan. Populasi madidihang di perairan terancam akibat terjadinya degradasi daerah penangkapan. Hal ini disebabkan oleh penangkapan yang tidak terkontrol, sehingga mengakibatkan jumlah populasi madidihang yang terdapat di perairan Provinsi Aceh semakin mengecil pada ukuran panjang. Upaya dalam menjaga potensi madidihang di perairan Provinsi Aceh dengan menghitung komposisi ukuran hasil tangkapan berdasarkan waktu (temporal) dan daerah penangkapan (spasial), hal ini dapat menjadi langkah dalam menekan tingkat pemanfaatan sumberdaya madidihang belum layak tangkap secara besar-besaran. Metode dalam penelitian meliputi pengumpulan data primer dan data sekunder, serta análisis hasil tangkapan berdasarkan komposisi ukuran panjang untuk menentukan daerah penangkapan madidihang. Tujuan mengetahui komposisi ukuran panjang hasil tangkapan berdasarkan lokasi dan waktu penangkapan, agar dapat menentukan jenis alat tangkap yang dioperasikan untuk menghindari tertangkapnya ikan-ikan madidihang belum layak tangkap (ilegal size). Penentuan daerah penangkapan (fishing ground) madidihang secara spasial dan temporal dapat diinterpretasikan dalam peta daerah penangkapan. Optimasi penangkapan madidihang lebih efektif di perairan Pulau Aceh, Samudera Hindia, dan perairan Pulau Rondo.Kata kunci: Daerah penangkapan ikan, Provinsi Aceh, , Thunnus albacares, tuna Madidihang

Aedes-borne arboviruses have emerged as an important public health problem throughout the world where Aedes spp. are the vectors responsible for this disease. This study was conducted to identify the material types of breeding containers of Aedes spp. larvae as one of the baseline data for Kuta Alam SubDistrict and to suggest suitable Aedes prevention and control strategies. Larvae were identified morphologically and larval indices were also calculated. A total of 2436 containers out of 14 types of containers observed during study periods. The positive breeding containers were surveyed and classified into indoors and outdoors environments. The material of positive breeding container types found was classified into six categories; cement, ceramic, plastic, metal, rubber and glass. As many as 450 of the 2436 potential containers included in the survey, half of the positive containers are cement and plastic materials which represent 80% of the total both in rural and urban areas, indoors and outdoors environment. It was followed by ceramic (15%), metal (3%) and rubber (1%). Aedes spp. larvae were most abundant in cement and plastic material. Therefore, community mobilization towards eliminating human-made containers, either indoor or outdoor, is vital for the dengue control program.

This study was aimed to identify possible critical points of Escherichia coli (E. coli ) O157:H7, a pathogenic agent, contamination in aceh cattle breeding centre. For this purpose, samples were collected from cattle faeces, hand of workers (animal keepers), and water sources in the farm using cross-sectional approach. A number of 85 samples of cattle faecal swab were collected randomly from the animals in the breeding centre. The samples of swab of hand of all workers (15 persons) were collected before and after work. Then, the water sources from 11 cattle house locations in the breeding centre were collected. The water sources were divided into three different locations, namely the water containers, taps, and water puddle on the floors. At each source a number of 11 samples were collected. Isolation of E. coli was conducted on Eosin Methylene Blue Agar (EMBA), followed by identification on Sorbitol MacConkey Agar (SMAC). Then Molecular subtyping of E. coli O157:H7 genes was conducted using multiplex-PCR analysis. Data were analysed descriptively. The results of this study showed that 72 samples (85 %) among 85 samples were positive for E. coli and the rest of samples were positive for other types of bacteria. Sample isolation from swabs of hand was found 3 positive E. coli before work and 1 positive E. coli after work from 15 workers. The most potential water sources for E. coli contamination were the water in taps, and water puddle on the floor of cattle houses. Then, two of samples of E. coli isolated from rectal swab were confirmed as E. coli O157:H7 using PCR test, based on the presence of stx2 gene. In conclusion, the risk of presence of E. coli as zoonotic agents of E. coli O157:H7 in aceh cattle as well as from the farm workers and surrounding area are high. An appropriate control strategy is needed to apply in the aceh cattle farm to prevent from E. coli O157:H7 outbreak in the future.

Snapper (Lutjanus sp.), which is an important marine fish resource, is known to have relatively low movement activity and form relatively small groups. These characteristics make these fish susceptible to various diseases caused by pathogenic bacteria. This study aims to identify the pathogenic bacterial species that contaminate snapper based on 16S rRNA gene analysis. The samples used in this study were the pathogenic bacterial isolates BPK-1 and BPK-2 which had previously been isolated from snapper fish originating from Banda Aceh waters. The bacteria were previously grown in nutrient broth (NB) media, then DNA genome from the bacteria was isolated using Presto ™ Mini g DNA Bacteria Kit, followed by PCR amplification of 16S rRNA gene. PCR products were sequenced and nucleotide sequence data were analyzed by bioinformatics software to construct phylogenetic tree. The 16S rRNA gene sequences of the two isolates BPK-1 and BPK-2 were two different bacterial species. BPK-2 isolate has similarities with Aeromonas sp. with a similarity percentage of 96.69%. While the BPK-1 isolate has similarities with Aeromonas salmonicida with a similarity percentage of 85.88%, so it is suspected that it is a novel species that needs further research.