An investigation of root rot in Acacia mangium plantations in Indonesia generated over 1000 fungal isolates from field surveys at six locations. The majority of isolates were identified as Ganoderma philippii or Ganoderma mastoporum by species-specific PCR, but this still left 274 isolates to be identified. As barcoding initiatives for fungi are still in the early stages, to assist identification of these isolates in this way we developed a database of sequences based on identified sporocarps and also searched public DNA sequence databases for sequences with high similarity. The database of sequences from sporocarp collections is more limited than public DNA databases. This resulted in more reliance on public DNA databases for a high proportion of taxa. Several pathogenic species were identified, with Phellinus noxius occurring at several locations and on both A. mangium and Eucalyptus pellita. Ganoderma steyaertanum was isolated from A. mangium roots and Rigidoporus microporus from E. pellita roots, each at a single location only. Potential pitfalls of using public DNA databases for fungal identification are discussed and methods to avoid these and increase confidence in the identification are presented.

DNA sequence variation in the rDNA internal transcribed spacers (ITS) was exploited to design species-specific primers for Ganoderma philippii and Ganoderma mastoporum, two fungi associated with red root rot in Acacia mangium and other perennial crops. Specificity was verified against five other Ganoderma species, 34 other basidiomycete species and two ascomycete species. The PCR tests assisted in the identification of 822 fungal isolates obtained from A. mangium and Eucalyptus pellita plantations in Indonesia. These tests have provided a high level of confidence in the identification of the major root rot pathogens, information that can be used to support the development of management options for their control in A. mangium plantations.

Three progeny trials of nyawai (Ficus variegataBlume ABSTRAKTiga plot uji keturunan nyawai (Ficus variegata Blume) dibangun di Mangunan, Bantul, Yogyakarta. Uji keturunan dikelompokkan dalam tiga sub galur berdasarkan sumber provenansinya: Lombok, Banyuwangi dan Cilacap-Pangandaran. Masing-masing sub galur dibangun dalam Rancangan Acak Lengkap Berkelompok menggunakan sebanyak 15-19 famili. Penelitian ini bertujuan untuk mengetahui variasi pertumbuhan dan parameter genetik tiga sub galur uji keturunan pada umur 6 dan 12 bulan setelah penanaman. Analisis varians digunakan untuk mengetahui pengaruh famili terhadap sifat tinggi dan diameter. Selanjutnya komponen varians pada masing-masing sifat digunakan untuk menaksir nilai heritabilitas dan korelasi genetik. Hasil penelitian menunjukkan persen hidup sampai umur 12 bulan di atas 90% yang mengindikasikan jenis tanaman ini mampu beradaptasi dengan baik pada tapak penelitian. Famili menunjukkan pengaruh yang nyata seiring dengan bertambahnya umur tanaman. Kecuali sifat diameter pada sub galur Banyuwangi, taksiran nilai heritabilitas

Tree diversity in Asia's tropical and subtropical forests is central to nature-based solutions. Species vulnerability to multiple threats, which affect provision of ecosystem services, is poorly understood. We conducted a region-wide, spatially explicit assessment of the

Recent advances in DNA marker technologies have increased the use of single nucleotide polymorphism (SNP) as a genetic marker. We developed SNPs in Paraserianthes falcataria and arranged them for multiplexed DNA typing using single nucleotide primer extension (SNuPE). Seventeen sequence characterized amplified regions (SCARs) were developed from random amplified polymorphic DNA (RAPD). In 12 SCARs of them, a SNP which possessed high heterozygosity was selected, and three sets of multiplexed SNuPE analysis system with 4 SNPs were constructed. Estimating the discrimination power (DP) revealed that set A had the highest DP (0.968), followed by set B and set C. The ability to discriminate could be almost 100% (1.000 of DP) when all sets were used. This SNuPE system provides a practicable method for individual identification of this forest tree species.