ABSTRACT. The purpose of this work was to determine the diversity and population fluctuations of calliphorid flies in the Biological Reserve of Tinguá (ReBio-Tinguá), Nova Iguaçu, state of Rio de Janeiro, Brazil and to correlate their occurrence with the environmental variables of temperature, rainfall and relative air humidity. Specimens of Diptera were collected monthly between June 2002 and January 2005 using four traps placed at four points along a trail and exposed for 48 hours. The traps were baited with sardines and the trapped insects were stored in 70% alcohol. It was collected 8,528 calliphorids, thirteen species were identified among the blowflies including Laneela nigripes Guimarães 1977, Chrysomya megacephala (Fabricius, 1794 (Aldrich, 1922). No significant correlation was found between the abundance of blowflies and the temperature and relative air humidity. Only C. megacephala and C. albiceps showed a positive and significant correlation with rainfall. An analysis of grouping by month (UPGMA) revealed no seasonal difference in the composition of the community, indicating that the community of calliphorid flies is probably more influenced by the ecological niches occupied by each species than by the seasons of the year. KEYWORDS.Seasonal abundance, diversity, Atlantic Forest, blowflies. (Aldrich, 1922). Não foi encontrada correlação significativa entre a abundância dos califorídeos e a temperatura e umidade relativa do ar. Somente C. megacephala e C. albiceps apresentaram correlação positiva e significativa com a precipitação. A análise de agrupamento dos meses (UPGMA) mostrou que não houve diferença na composição da comunidade em relação à sazonalidade, indicando que a comunidade de califorídeos é provavelmente mais influenciada pelos nichos ecológicos ocupados por cada uma das espécies do que pelas estações do ano. RESUMO. Flutuação populacional de califorídeos (Diptera, Calliphoridae) na Reserva Biológica do

-Monthly collections were made using two traps 5 m apart exposed for 48h, containing sardines and installed at points: A -at the edge (500 m from the entrance of the Reserve); B -1200 m from the entrance and 1000 m inside the forest; and C -1700 m from the entrance and 500 m inside the forest. The purpose was to evaluate the abundance and richness of calliphorid species as a function of the environmental conditions using Pearson's correlation, compare the richness of the areas using ANOVA and Tukey's test, compare the abundances of the areas by the Kruskal-Wallis test, and also assess the possible infl uence of the anthropic presence. Rare, intermediary and common species were identifi ed. The collection totalized 8515 Calliphoridae belonging to 26 species, with a predominance of females. None of the 13 species considered common presented a correlation between abundance and temperature: only Cochliomyia hominivorax (Coquerel) and Chrysomya megacephala (Fabricius) were correlated with humidity and only Mesembrinella semihyalina Mello with precipitation. This parameter was the only climatic variable correlated with richness. The greatest abundance and richness of calliphorids occurred in September 2006. From the 13 common species, seven were considered synanthropic, indicating the effect of anthropic action in this site.

A biodiversidade é regulada por vários fatores, em várias escalas, sendo esses fatores locais ou regionais (Moreno 2001). Em uma mesma área geográfica, a diversidade reflete a coexistência entre os organismos, que interagem através da competição por utilizarem as mesmas fontes ou o mesmo ambiente (Whittaker 1967). Em ambientes em equilíbrio competitivo, a diversidade total de espécies apresenta-se normalmente baixa e as espécies são suficientemente diferentes ABSTRACT. Analysis of Calliphoridae (Diptera) fauna in Reserva Biológica do Tinguá, Nova Iguaçu, Rio de Janeiro. The present study investigates the calliphorid fauna in the Biological Reserve of Tinguá using faunistic indices. Monthly samplings were carried out using traps containing sardines that were exposed for 48 hours at the sites: A, at the border of the forest; B, 1,000 m towards the interior of the forest; and C, 500 m towards the interior of the forest. The objective was to study the Calliphoridae fauna in forest environments using faunal indices. Jackknife 2 at site B produced a higher value, however, indicating that it was possible to collect five other species in the reserve and three more species at A and C. Diversity was greatest at B, but evenness was similar at the sites. B and C showed a greater level of similarity in species composition (dendogram); L. nigripes and Mesembrinella bellardiana, which are the most important species at these two sites, appeared grouped together. Chrysomya albiceps, Chrysomya megacephala and Hemilucilia semidiaphana, the main species at Site A, also appear together.KEYWORDS. Blow fly; diversity; evenness; variety.RESUMO. Análise faunística de Calliphoridae (Diptera) da Reserva Biológica do Tinguá, Nova Iguaçu, Rio de Janeiro. O presente estudo investiga a fauna de califorídeos na Reserva Biológica do Tinguá usando como meios os índices faunísticos. Realizaram-se coletas mensais com armadilhas contendo sardinha expostas por 48 horas, nos pontos: A-borda da mata; B-1000 metros para interior da mata; C-500 metros para interior da mata. Objetivou-se estudar a entomofauna califorídica em ambiente florestal através de índices faunísticos.

Samples were collected every month in three different sites of the Reserva Biológica do Tinguá, Brazil: site A was located on the border of the forest and sites B and C were located 1,000 and 500 m, respectively, towards the forest interior. The objective was to determine edge effects on a fragment of the Atlantic Forest. The greatest species richness was observed in sites A and B (23 species), compared with site C (16 species). Site A showed the greatest abundance and constancy, independent of the degree of synanthropy. Asynanthropic species were more abundant and constant in sites B and C. Site B showed the greatest diversity; and sites A and B showed the greatest similarity of populations. There was no significant correlation between Calliphoridae richness and canopy openness except in site C. Richness and abundance were positively correlated with subwood density, except for richness in site B.Keywords: edge effect, diversity, constancy index. Efeitos da fragmentação florestal sobre a dipterofauna (Calliphoridae) na Reserva Biológica do Tinguá, Nova Iguaçu, RJ ResumoForam realizadas coletas mensais em três pontos da Reserva Biológica do Tinguá: o ponto A estava localizado na borda da mata, e os pontos B e C estavam localizados, respectivamente, a 1000 e 500 m no interior da mata. Objetivou-se determinar os efeitos de borda em um fragmento da Mata Atlântica. A riqueza de espécies foi maior nos pontos A e B (23 espécies), em relação ao ponto C (16 espécies). No ponto A, ocorreu maior abundância e constância de califorí-deos, independente do seu grau de sinantropia. Nos pontos B e C, as espécies assinantrópicas foram mais abundantes e constantes. O ponto B apresentou a maior diversidade registrada. Os pontos A e B apresentam maior similaridade quanto às populações. Não houve significativa correlação entre riqueza de califorídeos e abertura do dossel, exceto no ponto C. Riqueza e abundância correlacionaram-se positivamente com densidade do sub-bosque, exceto para riqueza no ponto B.Palavras-chave: efeitos de borda, diversidade, índice de constância.

Abstracti mb_970 137..146Hexamerins and prophenoloxidases (PPOs) proteins are members of the arthropod-haemocyanin superfamily. In contrast to haemocyanin and PPO, hexamerins do not bind oxygen, but mainly play a role as storage proteins that supply amino acids for insect metamorphosis. We identified seven genes encoding hexamerins, three encoding PPOs, and one hexamerin pseudogene in the genome of the parasitoid wasp Nasonia vitripennis. A phylogenetic analysis of hexamerins and PPOs from this wasp and related proteins from other insect orders suggests an essentially order-specific radiation of hexamerins. Temporal and spatial transcriptional profiles of N. vitripennis hexamerins suggest that they have physiological functions other than metamorphosis, which are arguably coupled with its lifestyle.

ABSTRACTthe Calliphoridae are flies of great ecological, medical and sanitary importance because they are decomposers of organic matter, mechanical vectors of pathogenic agents, and causers of myiasis. this paper attempts to ascertain the diversity of Calliphoridae in the tinguá Biological reserve and correlate meteorological data (temperature, relative humidity of the air, and precipitation) with the occurrence of these flies. the study was conducted at a site in the tinguá Biological reserve, located in the municipality of nova iguaçu, state of rio de Janeiro, Brazil. four traps were set up using chicken viscera as bait. the experiment was conducted over the period of May 2001 to April 2002, with two monthly collections. the captured flies were killed with ether and conserved in 70% alcohol and identified in the laboratory of Diptera studies (unirio) and the laboratory of vector insect Biology and Control (fioCruz), both headquartered in the city of rio de Janeiro, rJ, Brazil. A total of 1,987 insects were captured, 37.5% belonging to the family Calliphoridae, 29.4% to Muscidae, 16.0% to sarcophagidae, and 17.1% to other families. the most representative species found was Phaenicia eximia (47.0%), followed by Hemilucilia semidiaphana (23.6%), Mesembrinella bellardiana (13.7%), Hemilucilia segmentaria (7.5%), Chloroprocta idioidea (4.9%), Chrysomya albiceps (1.2%), Chrysomya megacephala (0.9%), Phaenicia sericata (0.6%), Eumesembrinella sp. (0.5%), and Chrysomya putoria (0.1%). large numbers of Calliphoridae were noted in May, June, september and January, coinciding with low rainfall and an average temperature of 21.8 to 27.0 °C. A negative correlation was found between the number of Calliphoridae captured and meteorological factors.Keywords: population dynamics, biological reserve, Calliphoridae. ReSumo

Myiases are infestations with dipteran larvae in both necrosed and living tissues, the food source of these insects. These illnesses occur in warm humid climates, and are most frequent in developing countries. We assessed the epidemiological aspects and the influence of climate on the occurrence of myiases and the bioagents in patients admitted to the federal Hospital do Andaraí in Rio de Janeiro from February 2007 to 2008. The influence of abiotic factors (temperature, humidity, and rainfall) on the incidence of myiases was investigated by using the Pearson's correlation test. Of the 40 patients studied, the prevalence of myiases was higher in adults, particularly in the 40 to 65 year-old (37.5%) African descent males (57.5%). Most of the injuries were caused by trauma (62.5%). Some patients made use of licit (50%) and illicit drugs (17.2%). The occurrence of myiases was not affected by the abiotic factors investigated. The cases reported here were treated in only one hospital, indicating that the disease is relatively common. The most frequent bioagent was Cochliomyia hominivorax (Coquerel), but Cochliomyia macellaria (Fabricius), Chrysomya albiceps (Wiedemann) and Dermatobia hominis (Wiedemann) (Diptera: Calliphoridae) were also detected causing myiases. Chrysomya albiceps is an exotic etiologic agent of myiases.

Some microhymenopterans are parasitoids of flies of forensic importance. Their parasitic habit can alter the duration of post-embryonic development of these flies, altering the postmortem interval. In order to analyze possible alterations occurring during the immature development period of Nasonia vitripennis, this study tested different quantitative associations between female parasitoids and pupae of Chrysomya megacephala, which were defined by: