This research was conducted to study hormonal and metabolic statuses and to determine ultrasound criteria for early diagnosis of fetal growth retardation syndrome (FGRS) in dairy cows of Black-motley (n = 41) and Red-motley (n = 56) Holstein breeds. Concentrations of progesterone, testosterone, 17β-estradiol, dehydroepiandrosterone sulfate, cortisol, and triiodothyronine in blood serum were assessed by immune-enzyme analysis. Concentrations of plasma L-ascorbic acid and nitrogen oxides, serum immune globulin, middle molecular peptides, and bactericidal and lysozyme activity were determined by spectrophotometry. On days 38-40 and 60-65 of gestation, FGRS in cows was associated with hypoprogesteronemia, decrease of adrenal and thyroid gland functions, decreased nitric oxide synthesis, low immune responsiveness, and high endogenous intoxication. In conclusion, body length less than 16 mm on days 38-40 and less than 45 mm on days 60-65 are the criteria for designating underdevelopment of embryos and fetuses in dairy cows. FGRS genesis in dairy cows is determined by the embryo's and fetus's malnutrition at the early implantation and placentation stages, caused by the imbalance of endocrine regulation, nitric oxide systems, and endogenous intoxication.
The study aimed to assess the possibilities of applying electronic nose device for early diagnosis of postpartum endometritis in cows using secretions. The experiment included four clinically healthy cows, four cows with endometritis. Samples of secretions were obtained on days 1,3,6,8 after analysis of their odours was carried out with MAG-8 device (Russia). As and measuring array, there were used eight sensors based on piezoelectric crystals frequency of 10,0 MHz and film sorbents on electrodes that respond to compounds of C2-C4 alcohols, linear and cyclic ketones, aldehydes, aliphatic amines, and sulfur-containing organic compounds, acids, ammonia. It was found that during the first three days after calving in cows with postpartum endometritis in comparison with healthy animals, the area of the visual response of the sensors was exceeded by 28,4% the content of alcohols, ketones of linear a structure by 5,2-6,0%, aliphatic acids were less by 14,3% and alkyl amines by 9,2%. On 6-8 days after calving, in the clinical manifestation of endometritis, an excess of the “visual imprint” by 6.1 times (p <0.001), a decrease in the content of aliphatic acids by 22.7% (p < 0.05), and an increase in the concentration of sulfur-containing organic compounds - by 4.9%. Therefore, the electronic nose system can distinguish between the composition of the volatile compounds of the cervical postpartum secretions with the normal and pathological course of the postpartum period.
A significant problem in the dairy farming industry is the decrease in the livestock reproductive capacity due to the ovaries physiological functions violation. This study examines the lipids, proteins and carbohydrates metabolism state, depending on the sexual cyclicity recovery in animals degree, while observing adequate feeding. For the experiment, 16 new-calving Holstein-Friesian cows with an average annual milk yield of 9.5 thousand kg were selected, divided into 2 equal groups, depending on the ovulatory function resumption. All animals received a balanced diet. Blood was taken on days 6, 12, 19, 40 and 68 after calving. Blood serum was separated and the total protein and individual fractions content, as well as the creatinine, urea, total lipids, cholesterol, triglycerides, glucose amount, were determined. To determine the hormonal status, the content of estradiol and progesterone was examined. The reproductive organs condition was assessed using transrectal palpation and using an ultrasound apparatus. We found that cows with ovarian dysfunction are characterized by protein and glucose globulin fraction decreased concentrations in the blood, increased creatinine content and hypothalamus-pituitary-gonad system hormone-producing function inhibition. With an accuracy of 87.5%, the glucose threshold level in the blood serum was revealed, at which ovarian dysfunction can be predicted: a decrease in fertility in cows is observed at a rate of <2.2 mmol/l. Taking into account the previous studies results, further research expediency on finding the hormonal and metabolic status relationship with ethology typological features and the central nervous system state is substantiated.
Chronic endometritis is diagnosed on average in 18.1 % of infertile cows. With chronic endometritis, the uterus has a pronounced cavity of 18.4±0.9 mm (11-35 mm), while the wall thickness of the uterus is on average 8.7±0.37 mm. Morphometrically chronic endometritis is characterized by a decrease in the fraction of functionally active elements of the endometrium (integument epithelium, uterine glands, blood vessels) to 12.17 %. In the development of chronic endometritis, cows compared with clinically healthy animals showed an increase in leukocyte content by 12.2 %, monocytes by 2.5 times, creatinine by 29.7 %, average molecular peptides by 25.0 %, and endogenous intoxication index by 25.4 %, circulating immune complexes by 38.5 %, with a decrease in the bactericidal and lysozyme activity of blood serum, respectively by 10.7 and 12.9 % and phagocytic activity of leukocytes by 12.4 %. The effectiveness of the application of the developed method for the treatment of chronic endometritis, involving the use of aminoseleton five times with a 72-hour interval at a dose of 30, 35, 40, 45 and 50 ml, magestrofan on the 1st and 13th days at a dose of 2 ml, uterotone on 3rd, 4th and 5th days at a dose of 10 ml and intrauterine administration of primalact on 1st-3rd days at a dose of 20 ml, is 88.0 %. Recovery of animals after the treatment is accompanied by a decrease in the inflammatory response, endogenous intoxication, normalization of liver and kidneys, an increase in overall non-specific resistance and a decrease in uterine microbial contamination.
It was shown that intrauterine growth restriction and embryo death at early stages of formation are connected with endocrine, immune, and metabolic disorders in the organisms of inseminated animals and formation of the "mother-embryo-fetus" system (3,5,6).Macro-and microelements play an important role in providing reproductive functions in animals and humans (7-12). Calcium, phosphorus, and magnesium together provide the course of energetic and plastic processes in the organs of the reproductive system, activity of uteroplacental and fetoplacental blood flows, and development of the fetus's bone tissue. Zinc, copper, manganese, selenium, and iodine in the composition of enzymes, hormones, and
The article presents the material on studying the efficacy of the preparation of recombinant interferons containing granulocyte-macrophage colony-stimulating factor (GM-CSF) for the prevention of the post-estral uterine bleeding in dairy cows, accompanied by a retardation in ovulation of the dominant follicle. It has been found that a single administration of the drug containing GM-CSF in its composition to cows at a dose of 5.0 ml during insemination, contributes to the ovulatory functional recovery in 86.7% of animals, and reduces the period from calving to fertilization by 27.7 – 37.8 days. The introduction of the recombinant interferon preparation to cows provides activation of the cellular link in the immune defense, normalization of the blood hormonal-cytokine profile, recovery of the ovulatory function in the genital gonads. This is manifested by an increase in the serum lysozyme activity – by 29.5%, leukocyte phagocytic activity – by 12.4%, phagocytic index – by 38.8%, phagocytic number – by 58.8%, progesterone – by 59.9%, estradiol – by 60.0%, testosterone – by 59.3%, DHEA-S – by 56.8%, IL-4 – by 42.5%, IL-10 – by 53.4%, with a decrease in the content of IL-2 – by 40.4% and IL-1β – by 37.2%.
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