The association between human papillomavirus type 16 (HPV16) and oral cancer has been widely reported. However, detecting anti-HPV antibodies in patient sera to determine risk for oral squamous cell carcinoma (OSCC) has not been well studied. In the present investigation, a total of 206 OSCC serum samples from the Malaysian Oral Cancer Database & Tissue Bank System, with 134 control serum samples, were analyzed by enzyme-linked immunosorbant assay (ELISA) to detect HPV16-specific IgG and IgM antibodies. In addition, nested PCR analysis using comprehensive consensus primers (PGMY09/11 and GP5+/6+) was used to confirm the presence of HPV. Furthermore, we have evaluated the association of various additional causal factors (e.g., smoking, alcohol consumption, and betel quid chewing) in HPV-infected OSCC patients. Statistical analysis of the Malaysian population indicated that OSCC was more prevalent in female Indian patients that practices betel quid chewing. ELISA revealed that HPV16 IgG, which demonstrates past exposure, could be detected in 197 (95.6%) OSCC patients and HPV16-specific IgM was found in a total of 42 (20.4%) OSCC patients, indicating current exposure. Taken together, our study suggest that HPV infection may play a significant role in OSCC (OR: 13.6; 95% CI: 3.89-47.51) and HPV16-specific IgG and IgM antibodies could represent a significant indicator of risk factors in OSCC patients.
The Cherenkov light lateral distribution function (LDF) was simulated with the CORSIKA code, in the energy range (10 13 -10 16 ) eV. This simulation was performed for conditions and configurations of the Tunka EAS Cherenkov array for two primary particles (p and Fe). Basing on the simulated results, many approximated functions are structured for two primary particles and different zenith angles. This allowed us to reconstruct the EAS events, which is, to determine the type and energy of the primary particles that produced showers from signal amplitudes of Cherenkov radiation which measured with Tunka Cherenkov array experiment. Comparison of the calculated LDF of Cherenkov radiation with that measured at the Tunka EAS array shows the ability for identifying of the primary particle that initiated the EAS cascades determining of its primary energy around the knee region of the cosmic ray spectrum.
In the past two decades, COMSOL Multiphysics Software Package have emerged as a powerful tool for simulation, particularly in Nanotechnology and most importantly in biomedical application and various application involving fluid and solid interactions. Compared with conventional component or system design, distinctive advantages of using COMSOL software for design include easy assessing to the significant parameters in various levels of design, higher throughput, process monitoring with lower cost and less time consuming [1,. This review aims to summarize the recent advancements in various approaches in major types of micro fluidic systems simulations, design application of various COMSOL models especially in biomedical applications. The state-of-the-art of past and current approaches of fluid manipulation as well as solid structure design fabrication was also elaborated. Future trends of using COMSOL in nanotechnology, especially in biomedical engineering perspective.
Cardiovascular disease (CVD) is a major threat to global health, estimated to be the cause 30 % (17.3 million in 2008) of deaths every year, and the number of deaths caused by CVD is expected to increase further, reaching 23.3 million by 2030. Hence, there is a growing demand for simpler sample extraction, rapid screening results, and intervention of the subsequent analysis in emergency units. In this paper, we reviewed CVD biomarkers in blood- and saliva-based specimens. The history of cardiac biomarkers indicates that in the beginning, cardiac troponin I (cTnI) was a widely accepted 'gold standard' marker due to its high specificity and selectivity. Considering the advantages of salivary-based cardiac biomarkers, we examined correlations between non-invasive (salivary) and invasive (blood) diagnoses, and it was found that C-reactive protein (CRP) provides a better correlation. Despite the low abundance of salivary CRP, several reports displayed the detection limit down to pg/ml using existing technologies. Thus, salivary CRP has the potential to be used for future forefront diagnostics for the early assessment of cardiac risks.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.