ABSTRAKPenelitian ini bertujuan mengetahui pengaruh injeksi media kultur embrio supernatan ke dalam uterus mencit 2 hari sebelum transfer embrio (TE) terhadap angka implantasi, yang diindikasikan oleh adanya embrio dan leukemia inhibitory factor (LIF) pada uterus. Mencit jenis Swiss Albino dibagi menjadi 2 kelompok yakni kelompok perlakuan dan kelompok kontrol, masing-masing terdiri atas 30 mencit. Kelompok perlakuan mendapat injeksi media kultur embrio supernatan (MKES) dalam uterus 2 hari sebelum TE sedangkan kelompok kontrol mendapat injeksi media kultur embrio (MKE) dalam uterus 2 hari sebelum TE. Dua hari setelah TE (h6 kebuntingan), mencit diperfusi dengan menggunakan larutan para formaldehid 4% dan diambil uterusnya. Uterus diproses untuk blok parafin dan dipotong dengan mikrotom setebal 5 µm. Preparat dideparafinisasi dan diproses imunohistokimia dengan kit ABC dan antibodi LIF. Preparat diperiksa di bawah mikroskop untuk melihat implantasi embrio, distribusi LIF, dan jumlah LIF di desidua dan non-desidua. Angka implantasi antara kelompok perlakuan dan kelompok kontrol masing-masing adalah 52,77 dan 40,88% (P<0,05). Jumlah LIF desidua pada kelompok perlakuan dan kelompok kontrol masing-masing adalah 12,83 dan 8,83 (P<0,05) sedangkan jumlah LIF di non-desidua antara kelompok perlakuan dan kelompok kontrol masing-masing adalah 8,00 dan 4,50. Dapat disimpulkan bahwa injeksi media kultur embrio supernatan 2 hari sebelum TE dapat meningkatkan angka implantasi pada mencit. ____________________________________________________________________________________________________________________ Kata kunci: kultur embrio supernatan, angka implantasi, leukemia inhibitory factor ABSTRACTThe research was conducted to examine the effect of supernatant embryonic media culture injection into mice uterine at 2 days prior to embryo transfer implantation rate, indicated by the presence of implanted embryos and leukemia inhibitory factor (LIF) PENDAHULUANKomunikasi antara embrio dan endometrium (crosstalk) memegang peranan penting dalam keberhasilan implantasi dan kebuntingan. Hal ini dimulai sejak terjadinya embrio dan perjalanan dari saluran tuba Fallopii sampai implantasi pada endometrium. Embrio menghasilkan beberapa faktor selama perkembangannya dan memberi sinyal keberadaannya kepada organ maternal. Dalam 1-2 hari setelah fertilisasi dapat dideteksi adanya early pregnancy factor (EPF) pada sirkulasi darah maternal yang mempunyai sifat immunosuppresive dan berhubungan dengan proliferasi sel dan pertumbuhan (Fan dan Zheng, 1997).Saat terjadinya implantasi embrio, endometrium harus dalam keadaan kondusif untuk memungkinkan perlekatan, implantasi, dan pertumbuhan awal plasenta.Salah satu sinyal dari embrio yang memodulasi reseptor endometrium adalah human chorionic gonadotropin (hCG). Hormon hCG diproduksi oleh blastosis pada hari ke 7-8 pasca fertilisasi. Substansi lain yang disekresi embrio sebelum implantasi adalah platelet activating factor (PAF) yang mungkin bagian dari aktivitas immunosuppressive untuk menginduksi to...
Indonesia sangat kaya dengan keanekaragaman flora yang potensial untuk terapi herbal, salah satunya tanaman koro benguk (Mucuna pruriens) yang bijinya bisa diolah menjadi tempe sebagai konsumsi harian masyarakat di sekitar Yogyakarta dan Jawa Tengah. Biji koro benguk diketahui mengandung L-3,4-dihydroxyphenylalanine (L-DOPA) tinggi dan berpotensi menjadi agen neuroprotektor pada penyakit Parkinson. Penelitian ini bertujuan untuk menguji kandungan L-DOPA mulai dari biji koro benguk segar, rebus, dan bahan olahannya yaitu tempe benguk. Uji dilakukan pada biji koro benguk mentah kulit berwarna putih (BR D) dan hitam (BR A) asal Wonogiri, Jawa Tengah, serta biji koro benguk mentah kulit berwarna putih (KP C), biji koro benguk yang sudah direbus dua kali, dan tempe benguk asal Kulon Progo, Yogyakarta. Sampel diektraksi menggunakan pelarut etanol dan n-propanol, kemudian dianalisis dengan teknik high-performance liquid chromatography (HPLC) untuk melihat kadar kandungan L-DOPAnya. Hasil penelitian menunjukkan, kadar L-DOPA tertinggi (8,56%) ditemukan pada biji koro benguk mentah dengan warna kulit putih asal Wonogiri yang diekstraksi menggunakan pelarut etanol, sedangkan kadar L-DOPA terrendah (0,016%) ditemukan pada sediaan tempe yang diekstraksi dengan n-propanol asal Kulon Progo. Secara umum, ekstraksi menggunakan pelarut etanol memberikan hasil kadar L-DOPA yang lebih tinggi dibandingkan dengan pelarut n-propanol. Semua ekstraksi sampel, mulai biji koro benguk segar, rebus sampai bentuk tempenya mengandung L-DOPA, dengan kadar tertinggi terdapat pada biji koro benguk segar berkulit putih asal Wonogiri, Jawa Tengah yang diektraksi menggunakan etanol, sedangkan kadar terendah dijumpai pada tempe benguk dari Kulon Progo, Yogyakarta yang diekstraksi menggunakan n-propanol.
Aim:n-Propanol extracts from fresh, boiled, and fermented seeds were studied to evaluate their neuroprotective effects in a Parkinson’s disease (PD) rat model, based on the total number of dopaminergic (DA) neurons in the substantia nigra pars compacta (SNpc).Materials and Methods:Rats were induced with paraquat dichloride at a dosage of 7 mg/kg BW intraperitoneally twice a week and at the same time supplemented with extract at a dosage of 70 mg/kg BW orally every day for 3 weeks. On the 24th day, all rats were perfused and fixed with 4% paraformaldehyde. The left part of the SNpc was processed for immunohistochemical staining with tyrosine hydroxylase (TH)-antibody. The total number of DA neurons in SNpc was evaluated with a stereological method.Results:TH-immunoreactive cells found in the SNpc were identified as DA neurons. The average total number of DA neurons in the SNpc increased significantly in the PD rat model that was given an n-propanol extract of boiled and fermented seeds compared with a control PD rat model. Surprisingly, there was no significant difference in the average total number of DA neurons in SNpc between the PD rat model that was given n-propanol extract of fresh seeds and the control PD rat model.Conclusion:n-Propanol extract of boiled and fermented seeds could produce a higher neuroprotective effect against DA neuron than fresh seeds in a PD rat model.
Paraquat dichloride has been used by farmers as a herbicide to kill the grass. On the other hand, paraquat dichloride is harmful if enters to the body, causing Parkinson's disease, since it is disrupting dopamine production in the substantia nigra pars compacta or dopamine pathways Nigro striatal pathway. The study was done to fi nd out the histological changes of catecholaminergic neurons and Nigro striatal pathway caused by paraquat dichloride treatment in Wistar rats as a model of Parkinson's disease.Twenty-two Wistar rats 3,5 months old were divided into 4 groups, 5 rats each. Group I (control group) were injected with aquabidest, while groups II, III, and IV were injected intraperitoneally with paraquat dichloride in aquabidest, with the dosage 5 , 10 and 15 mg/kg bw respectively. The rats were injected once per week for 6 weeks. Three days after the last injection, the rats were anesthetized using xylasin (2 mg/kg) and ketamine (20 mg/kg) intramuscularly, and then were intracardiac perfused using physiological saline as prerinse solution, followed by 10% buffered formalin solution as a fi xative. After animals were fi xed, the brains were removed and embedded in paraffi n block and cut in 12 μm thickness for immunohistochemistry staining using tyrosine hydroxylase antibody. The results of staining then were observed under light microscope and analyzed descriptively.The results showed that the catecholaminergic neurons were distributed in the substantia nigra pars compacta in all treatment groups, however, the cell density were found decreased only in group IV. Catecholaminergic neurons appear in the bipolar and multipolar form, while dopamine 'Nigro striatal pathway' was found exist in all treatment groups. From our study, histologycally the decreased of catecholaminergic neurons is only found in rats that received paraquat dichloride in dose 15 mg/kg bw for 6 weeks.
Background: Angiogenesis is the process through which new blood vessels are formed from existing ones. This process plays an important role in supplying the oxygen and nutrients needed for cellular metabolism and eliminating cell debris during wound healing. Snail mucus can bind to several factors that stimulate angiogenesis, including vascular endothelial growth factor, platelet-derived growth factor, and fibroblast growth factor. The aim of this study is to observe changes in angiogenesis during the healing of wounds topically applied with snail mucus. Methods: Punch biopsy was performed on the back of male Wistar rats to obtain four wounds, and different concentrations of snail mucus were applied to each of these wounds. The animals were sacrificed on days 2, 4, and 7 to observe the extent of angiogenesis during wound healing by microscopy. Results: Two-way ANOVA showed differences in number of blood vessels formed (p = 0.00) and day of observation (p = 0.00) between groups. Post hoc Tukey’s HSD test showed that 24% snail mucus treatment does not significantly affect wound healing (p = 0.488); by contrast, treatment with 48% and 96% snail mucus demonstrated significant effects on angiogenesis (p = 0.01). Spearman’s test showed interactive effects between snail mucus concentration and day of observation on the extent of angiogenesis (p = 0.001, R = 0.946). Conclusion: Topical application of snail mucus gel can increase angiogenesis during wound healing in Wistar rat skin.
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