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Background and Aim: Java Island is one of the islands in Indonesia which has local sheep breeds with specific characteristics and native development geography in certain regions. This study aimed to determine the genetic profiles and maternal origin of six local sheep breeds on Java Island. Materials and Methods: This study was conducted by identifying the profiles of complete mitochondrial DNA (mtDNA) displacement loop (D-loop) region sequences on a total of 22 individual in six local sheep breeds on Java Island, including Javanese thin-tailed (JTT), Javanese Fat-Tailed (JFT), Batur (BTR), Wonosobo (WSB), Garut (GRT), and Priangan (PRG) sheep. The D-loop region was amplified using specific primers, and the polymerase chain reaction (PCR) was performed. The PCR products were purified and sequenced. Results: The mtDNA D-loop analysis identified 21 haplotypes in the analyzed 22 animals with 123 polymorphic sites (V) consisting of 60 singleton variable sites (S) and 63 parsimony informative sites (P). Within all breeds tested, the haplotype diversity, the average number of pairwise differences (K), and nucleotide diversity (Pi) were 0.99567, 25.36364, and 0.02153, respectively. The genetic distance (D) within groups and between groups was 0.001-0.006 and 0.004-0.036, respectively. The phylogeny resulted in the presence of two haplogroups (Hap), which are 5 Hap A and 16 Hap B. All JTT, JFT, BTR, and WSB breeds were in the same cluster in Hap B, whereas GRT and PRG breeds were in clusters in both Hap A and Hap B. Conclusion: The high genetic diversity in six local sheep breeds on Java Island suggests that they originated from different genetic sources. JTT sheep have closer genetic relationships to JFT, BTR, and WSB sheep, and they are close to European sheep, whereas GRT sheep have closer genetic relationships to PRG sheep. Both are closer to Asian sheep than to European sheep.

Indonesia has several breeds of goats as the source of genetic. This study aimed to characterize genetic of Indonesian goats. The phylogenetic relationship among and genetic variability within eight native Indonesian goats were analyzed using mtDNA D-loop region sequences. The results show that the length of complete sequences mtDNA D-loop in Indonesian goats are1212 bp. There are 68 polymorphic sites and 21 different haplotypes. Haplotype diversity and nucleotide diversity based on mtDNA D-loop in eight breeds of Indonesian goats are 0.5000-1.000 and 0.248-1.1595%, respectively. The value of genetic distance between groups of native Indonesian goats ranged from 0-0.011. Indonesia goat genetic distance value against wild goat Capra aegagrus (AB004082) is in the range 0.0038-0.045 while against Capra falconeri (AB044306) is 0.087-0.095. The results indicate that the genetic diversity of Indonesian goats is not abundant. Indonesian goats originated from one maternal. Maternal origin of Indonesian goats based on mtDNA D-loop is lineage B.

Aim: This study aimed to determine the genetic characterization and phylogenetic structure of Indonesian indigenous catfish using cytochrome B (Cyt B) sequences. Materials and Methods: The genomes of 26 catfishes caught from nine rivers from nine different geographical locations around Indonesia were analyzed. The tissue isolation method was used to isolate the total genome of the fishes. Furthermore, polymerase chain reaction was done to amplify the mtDNA Cyt B using the CytBF and CytBR primers. Following sequencing, the analysis of genetic variation and the phylogenetic relationship was performed using MEGA version X software. Results: Cyt B gene sequencing attained a total of 1139 nucleotides encrypting 379 amino acids for all samples. The ClustalW alignment program using MEGA X software revealed 395 substituted nucleotides, which then translated into 63 amino acid variation sites among all 26 samples. No amino acids in catfish BB were different compared to catfish PM, MP, and KR2,3. Catfish MS had one modified amino acid; KR1 and KS had two different amino acids; BF had 38 different amino acids; EM had 31 different amino acids; and BSBJ had 26 different amino acids compared to catfish BB. The most significant alteration of amino acids was between catfish EM and BF (49 amino acids). Conclusion: Indonesian catfish were divided into five clades based on the Cyt B gene. Samples KR and MP (Sumatra); MS and BB (Kalimantan); and PM (Java) were clustered with Hemibagrus nemurus and Hemibagrus wyckioides (Bagridae family). Samples from Kalimantan (KS) and one sample of KR (KR1) from Sumatra were clustered with Sperata seenghala and Hemibagrus spilopterus (Bagridae family). Samples from Java (BSBJ) were clustered with Pseudolais pleurotaenia (Pangasiidae family). Samples EM (Java) were together with Mystus cavasius (Bagridae family). Samples from West Papua were clustered with Potamosilurus latirostris (Ariidae family).

Abstract. Ibrahim A, Artama WT, Budisatria IGS, Yuniawan R, Atmoko BA, Widayanti R. 2021. Regression model analysis for prediction of body weight from body measurements in female Batur sheep of Banjarnegara District, Indonesia. Biodiversitas 22: 2723-2730. Bodyweight is an important aspect of livestock management. The present study was undertaken to estimate correlation coefficients between biometric traits and identify best predictor of body weight in female Batur sheep from body measurements. Data on body weight and body measurements (body length: BL, chest girth: CG and withers height: WH) were collected from 73 female Batur sheep in Batur Village, Banjarnegara District, Central Java Province, Indonesia. Batur sheep were grouped into 3 categories based on their age, namely groups <1.5 years, 1.5-2.5 years and >2.5 years. The data were analyzed using simple, multiple, and automatic linear regression methods using the SPSS computer software version 25 platform. The correlation coefficient, coefficient determination, adjusted coefficient determination, residual standard error, Akaike information criterion, Bayesian information criterion, and Akaike information criterion corrected were used to determine the best regression formula for the prediction of BW. The average BW (kg), BL (cm), CG (cm), and WH (cm) of 49.27, 63.11, 91.41, and 56.82, respectively was observed in the present study. The correlation coefficients of 0.433, 0.866, and 0.369 for BW with BL, CG, and WH were observed in the present study. The best prediction of BW using two predictors (BL and GC) was BW =-56.522 + 0.509BL + 0.843CG, followed by using three predictors (BL, CG, and WH) was BW =-57.897+ 0.505BL + 0.839CG + 0.034WH, and using the only one predictor (CG) was BW =-28.443 + 0.905CG. The study revealed that CG and its combination with other linear body measurements can effectively define the body weight in Batur sheep. However, the highest R2 of 0.782 was observed when CG and BL were used as predictors.

Aim: This study aimed to analyze the genetic variation and phylogenetic reconstruction of Indonesian indigenous catfish using mitochondrial cytochrome oxidase subunit III sequences. Materials and Methods: A total of 19 samples of catfish were collected from seven rivers (Elo [EM], Progo [PM], Kampar [KR], Musi [MP], Mahakam [MS], Kapuas [KS], and Bengawan Solo [BSBJ]) in five different geographical locations in Indonesia. The genome was isolated from the tissue. Mitochondrial DNA cytochrome oxidase subunit III was amplified using polymerase chain reaction (PCR) with CO3F and CO3R primers. The PCR products were sequenced and continued to analyze genetic variation and phylogenetic relationship using MEGA version 7.0 software. Results: Cytochrome c oxidase (COX)-III gene sequencing obtained 784 nucleotides encoding 261 amino acids. Sequenced COX-III gene fragments were aligned along with other catfish from Genbank using ClustalW program and genetic diversity among species was analyzed using the MEGA Version 7.0 software. Among all samples, there were substitution mutations at 78 nucleotide sites, and there were 14 variations in amino acids. Catfish from PM, KR, MP, and KS had the same amino acids as Hemibagrus nemurus (KJ573466.1), while EM catfish had eight different amino acids and catfish BSBJhad 12 different amino acids. Conclusion: Indonesian catfish divided into four clades. BBSJ Catfish were grouped with Pangasianodon gigas, EM catfish were grouped with Mystus rhegma, and KS catfish were grouped with Hemibagrus spilopterus, while catfish MS, KR, PM, and MP were grouped with H. nemurus.

Background and Aim:Excessive use of trypanocidal drugs can lead to cases of drug resistance. Multiple cases of resistance have been widely reported for drugs such as isometamidium chloride and diminazene aceturate. These cases deserve serious attention, especially in Indonesia, where the first case was recorded and where the molecular basis of trypanocidal drug resistance has never been evaluated. This study aimed to analyze the multidrug resistance protein (MRP) gene in Trypanosoma evansi isolates, sampled from Indonesia, by focusing on the phylogenetic relationship between these isolates and other Trypanosoma spp.Materials and Methods:A total of 88 blood samples were drawn from buffaloes in the Ngawi district, Indonesia. Animals infected with T. evansi were detected through the microhematocrit technique and Giemsa blood smear methods. Positive blood samples were used to inoculate in male mice (Mus musculus BALB-C strain) as an animal model for culturing the T. evansi. The genomic DNA of the blood taken from the T. evansi- infected mice was used for polymerase chain reaction amplification, sequencing, and phylogenetic analysis.Results:Two genes were analyzed; the first gene detected for T. evansi corresponded to Trypanosoma brucei with a homology of 99% and the second gene to Trypanosoma brucei gambiense, with a homology of 100%. These two genes of the MRP from T. evansi showed clear similarity to the MRPE and MRPA genes of the T. brucei ssp.Conclusion:The MRP gene is conserved on the subspecies level of T. brucei. Only few point mutations were found between various sequences, which mean that the proteins have the same structure. This is important to treat the parasite with the appropriate drugs in the future.

Sheep play an important role for Indonesian people, especially during Eid al-Adha celebration. There are several criteria in the selection of sheep as sacrificial animals. This study was aimed to observe consumer’s preferences for sheep attributes for Eid al-Adha celebration based on information gathered from roadside sheep traders. This study was conducted by using an in-depth interview and preferences ranking to 36 roadside sheep traders in Yogyakarta, Indonesia. The data were analysed descriptively. The results showed that the preferences for sheep criteria were body characteristics, body weight, body posture, age breed and other desirable criteria such as symmetrical testes, medium tail, intact earlobe and healthy hooves. Body characteristics favoured by consumers were horns, large body size, black ring eye pattern, teeth incisions, dense and nice hair, and white colour pattern of the body, respectively. It is concluded that the preferences for sheep attribute most demanded by consumers for Eid al-Adha celebration in Yogyakarta are body characteristics, wherein horned sheep are frequently requested criteria. Sheep price for Eid al-Adha is determined by religious and cultural criteria.