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Significance and Impact of the Study: The resuscitation-promoting factor Rpf2 of Rhodococcus erythropolis was expressed and purified. The purified protein showed muralytic activity and protease activity. The purified protein promoted the R. erythropolis cell growth. The addition of the recombinant Rpf2 protein significantly increased petroleum degradation efficiencies of the indigenous micro-organisms of the contaminated soils. The recombinant protein also increased the cultivable petroleum-degrading bacteria diversities of the contaminated soils. The protein also promoted isolation effect of the oildegrading bacteria from the contaminated soils. The Rpf2 of R. erythropolis is an important bacterial growth stimulating factor.

Codonopsis pilosula is a medicinal and edible herb with a rich nutritional value. In Gansu Province, China, its production quality and yield differ during the four seasons. Here, we investigated the differences in the microfloral composition and metabolic functions in the rhizospheric soil and roots of C. pilosula during the four seasons, and we also analyzed their dynamic and synergistic effects on C. pilosula growth and carbohydrate content change. The C. pilosula samples were analyzed for plant physiology, microfloral composition and metabolic functions in the rhizospheric soil and roots using high-throughput sequencing technology. Environmental indices including soil physiochemistry and meteorological conditions were also determined by the coupled chromatography–spectroscopy technique. The results revealed that the C. pilosula growth was affected by temperature, precipitation and light intensity, with the bacterial structures and functions of the soil and root samples showing obvious seasonal changes. Due to the diversity of microbial composition and community metabolic function, and the synergistic effect of microbial and environmental factors, there are significant differences in stress resistance, physiological status and metabolites of C. pilosula in different seasons. Furthermore, the change in seasons was significantly correlated with the quality and yield of C. pilosula. This study provides a scientific basis for soil improvement and the refinement of local Radix C. pilosula cultivation methods.