Promoting effect of the recombinant resuscitation promoting factors‐2 of Rhodococcus erythropolis on petroleum degradation and cultivable bacterial diversities of the oil‐contaminated soils

Abstract

Significance and Impact of the Study: The resuscitation-promoting factor Rpf2 of Rhodococcus erythropolis was expressed and purified. The purified protein showed muralytic activity and protease activity. The purified protein promoted the R. erythropolis cell growth. The addition of the recombinant Rpf2 protein significantly increased petroleum degradation efficiencies of the indigenous micro-organisms of the contaminated soils. The recombinant protein also increased the cultivable petroleum-degrading bacteria diversities of the contaminated soils. The protein also promoted isolation effect of the oildegrading bacteria from the contaminated soils. The Rpf2 of R. erythropolis is an important bacterial growth stimulating factor.

Enzymatic properties and biological activity of resuscitation-promoting factor B of Rhodococcus sp. (GX12401)

“…The application and research of Rhodococcus in the screening of new microorganisms is relatively rare. The existing research shows that the Rpf protein of Rhodococcus erythropolis can increase the diversity of culturable oil-degrading bacteria, and the culturable bacterial phyla increased from 9 to 13 and 16, respectively ( Fu et al, 2021 ); in the exploration of VBNC flora with denitrification ability, a total of 13 strains with heterotrophic nitrification ability were obtained after adding Rpf protein ( Su et al, 2019 ). The heterologously expressed RpfB protein in our laboratory has good enzymatic activity and can revive its own VBNC cells, so the application of RpfB protein will provide more possibilities for subsequent screening of new microbial species and functions.…”