Improved growth and metabolite accumulation in Codonopsis pilosula (Franch.) Nannf. by inoculation with the endophytic Geobacillu sp. RHBA19 and Pseudomonas fluorescens RHBA17

Zhu, Ning; Meng, Tongtong; Li, Shaowei; Yu, Chenqun; Tang, Dan; Wang, Yonggang; Leng, Feifan; Ma, Junsheng

doi:10.1016/j.jplph.2022.153718

Cited by 9 publications

(3 citation statements)

References 71 publications

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“…High concentrations of ROS can cause lipid peroxidation, membrane degradation, and damage the proteins, lipids, and nucleic acids in plants ( Fan, Subramanian & Smith, 2020 ). The plant antioxidant system, through the combined action of antioxidant enzymes such as superoxide dismutase (SOD), peroxidase (POD), catalase (CAT), and non-enzymatic antioxidants such as malondialdehyde (MDA), proline (Pro), and soluble proteins, eliminates ROS and maintains normal growth and development ( Zhu et al, 2022 ).…”

Section: Discussionmentioning

confidence: 99%

Beneficial endophytic fungi improve the yield and quality of Salvia miltiorrhiza by performing different ecological functions

Li,

Lin,

Qin

et al. 2024

PeerJ

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Background Endophytic fungi can enhance the growth and synthesis of secondary metabolites in medicinal plants. Salvia miltiorrhiza Bunge is frequently employed for treating cardiovascular and cerebrovascular ailments, with the primary bioactive components being salvianolic acid and tanshinone. However, their levels in cultivated S. miltiorrhiza are inferior to that of the wild herbs, so the production of high-quality medicinal herbs is sharply declining. Consequently, the utilization of beneficial endophytic fungi to improve the yield and quality of S. miltiorrhiza holds great significance for the cultivation of medicinal plants. Methods In this study, nine non-pathogenic, endophytic fungal strains were introduced into sterile S. miltiorrhiza seedlings and cultivated both in vitro and in situ (the greenhouse). The effects of these strains on the growth indices, C and N metabolism, antioxidant activity, photosynthesis, and content of bioactive ingredients in S. miltiorrhiza were then evaluated. Results The results showed that the different genera, species, or strains of endophytic fungi regulated the growth and metabolism of S. miltiorrhiza in unique ways. These endophytic fungi primarily exerted their growth-promoting effects by increasing the net photosynthetic rate, intercellular CO2 concentration, and the activities of sucrose synthase, sucrose phosphate synthase, nitrate reductase, and glutamine synthetase. They also enhanced the adaptability and resistance to environmental stresses by improving the synthesis of osmoregulatory compounds and the activity of antioxidant enzymes. However, their regulatory effects on the growth and development of S. miltiorrhiza were affected by environmental changes. Moreover, the strains that significantly promoted the synthesis and accumulation of phenolic acids inhibited the accumulation of tanshinones components, and vice versa. The endophytic fungal strains Penicillium meloforme DS8, Berkeleyomyces basicola DS10, and Acremonium sclerotigenum DS12 enhanced the bioaccumulation of tanshinones. Fusarium solani DS16 elevated the rosmarinic acid content and yields in S. miltiorrhiza. The strain Penicillium javanicum DS5 improved the contents of dihydrotanshinone, salvianolic acid B, and rosmarinic acid. The strains P. meloforme DS8 and B. basicola DS10 improved resistance. Conclusion Various endophytic fungi affected the quality and yield of S. miltiorrhiza by regulating different physiological and metabolic pathways. This study also provides a novel and effective method to maximize the effects of beneficial endophytic fungi by selecting specific strains to design microbial communities based on the different ecological functions of endophytic fungi under varying environments and for specific production goals.

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Section: Discussionmentioning

confidence: 99%

Beneficial endophytic fungi improve the yield and quality of Salvia miltiorrhiza by performing different ecological functions

Li,

Lin,

Qin

et al. 2024

PeerJ

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“…However, when C. pilosula was inoculated with two bacterial strains, Geobacillus sp. RHBA19 and Pseudomonas fluorescens RHBA17, significantly improvements in the root development, photosynthesis and growth of C. pilosula (Zhu et al 2022 ) were observed because these two strains produce indole acetic acid (IAA), siderophores, and several exoenzymes. These two strains also increased the metabolites of C. pilosula , i.e.…”

Section: Introductionmentioning

confidence: 99%

“…These two strains also increased the metabolites of C. pilosula , i.e. polysaccharides and LBT, and enhanced the activities of antioxidant enzymes such as chloramphenicol acetyl transferase (CAT), peroxidase (POD), and superoxide dismutase (SOD) (Zhu et al 2022 ). Exopolysaccharides isolated from endophytic strain 14-DS-1(DSPS) of C. pilosula exhibited anticancer activity by promoting infiltration and activation of macrophages and promoting the production of TNF-α and nitric oxide.…”

Section: Introductionmentioning

confidence: 99%

Antimicrobial activity and enzymatic analysis of endophytes isolated from Codonopsis pilosula

Lodi

Dong

Jiang

et al. 2023

FEMS Microbiology Ecology

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The roots of the medicinal plant Codonopsis pilosula (Franch.) Nannf (C. pilosula) possess most medicinal supplements. In Current research on C. pilosula root endophytes were isolated, identified, and evaluated for their antimicrobial activity against human pathogens such as Escherichia coli, Staphylococcus aureus, Bacillus subtilis, Salmonella typhi, and Pseudomonas aeruginosa and the fungi Candida albicans and Aspergillus niger. Endophytes C.P- 8 and C.P-20 exhibited very significant antimicrobial activity, the secondary metabolite of C.P-8 registered at retention time 24.075 by HPLC analysis. Significant minimum inhibitory concentration (MIC) of C.P-8 was exhibited at 250 µg/ml against Staphylococcus aureus and 500 µg/ml against Bacillus subtilis. Qualitative, quantitative analyses and partial purification of enzymes and purity was analysed by molecular weight determined by SDS‒PAGE of enzymes produced by C.P-20, amylase—64 kDa, protease—64 kDa, chitinase—30 kDa and cellulase—54 kDa. Optimum pH and temperature of the partially purified enzymes, was carried out. The partially purified enzymes from C.P-20 displayed maximum activity at pH 6–7 and temperatures of 40–45 °C. Moreover, the above endophytes will be useful tools for producing active enzymes and active bio-antimicrobial agents against human pathogens.

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PGPR-Mediated Breakthroughs in Plant Stress Tolerance for Sustainable Farming

Karnwal

Shrivastava

Al-Tawaha

et al. 2023

J Plant Growth Regul

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Improved growth and metabolite accumulation in Codonopsis pilosula (Franch.) Nannf. by inoculation with the endophytic Geobacillu sp. RHBA19 and Pseudomonas fluorescens RHBA17

Cited by 9 publications

References 71 publications

Beneficial endophytic fungi improve the yield and quality of Salvia miltiorrhiza by performing different ecological functions

Beneficial endophytic fungi improve the yield and quality of Salvia miltiorrhiza by performing different ecological functions

Antimicrobial activity and enzymatic analysis of endophytes isolated from Codonopsis pilosula

PGPR-Mediated Breakthroughs in Plant Stress Tolerance for Sustainable Farming

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