Bioremediation of the Petroleum Contaminated Desert Steppe Soil with <i>Rhodococcus erythropolis</i> KB1 and Its Effect on the Bacterial Communities of the Soils

Wei, Yanchen; Chen, Jixiang; Wang, Yonggang; Meng, Tongtong; Li, Mei

doi:10.1080/01490451.2021.1964111

Cited by 7 publications

(2 citation statements)

References 32 publications

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“…has been documented to adapt to hydrocarbon assimilation, ecological plasticity, biosafety in relation to humans, and also its effectiveness in situ, on-site, and during bioreactor treatment [ 6 ]. Rhodococcus erythropolis is an Actinobacterium with the versatility to grow in adverse abiotic and biotic stress conditions, including the wide range of complex compounds that it degrades [ 7 , 8 , 9 ]. Highly toxic and flammable, formaldehyde from a phenolic and melamine resin-manufacturing company was degraded and removed by R. erythropolis UPV-1 [ 10 ].…”

Section: Introductionmentioning

confidence: 99%

Biotechnological Key Genes of the Rhodococcus erythropolis MGMM8 Genome: Genes for Bioremediation, Antibiotics, Plant Protection, and Growth Stimulation

Afordoanyi,

Akosah,

Shnakhova

et al. 2023

Microorganisms

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Anthropogenic pollution, including residues from the green revolution initially aimed at addressing food security and healthcare, has paradoxically exacerbated environmental challenges. The transition towards comprehensive green biotechnology and bioremediation, achieved with lower financial investment, hinges on microbial biotechnology, with the Rhodococcus genus emerging as a promising contender. The significance of fully annotating genome sequences lies in comprehending strain constituents, devising experimental protocols, and strategically deploying these strains to address pertinent issues using pivotal genes. This study revolves around Rhodococcus erythropolis MGMM8, an associate of winter wheat plants in the rhizosphere. Through the annotation of its chromosomal genome and subsequent comparison with other strains, its potential applications were explored. Using the antiSMASH server, 19 gene clusters were predicted, encompassing genes responsible for antibiotics and siderophores. Antibiotic resistance evaluation via the Comprehensive Antibiotic Resistance Database (CARD) identified five genes (vanW, vanY, RbpA, iri, and folC) that were parallel to strain CCM2595. Leveraging the NCBI Prokaryotic Genome Annotation Pipeline (PGAP) for biodegradation, heavy metal resistance, and remediation genes, the presence of chlorimuron-ethyl, formaldehyde, benzene-desulfurization degradation genes, and heavy metal-related genes (ACR3, arsC, corA, DsbA, modA, and recG) in MGMM8 was confirmed. Furthermore, quorum-quenching signal genes, critical for curbing biofilm formation and virulence elicited by quorum-sensing in pathogens, were also discerned within MGMM8’s genome. In light of these predictions, the novel isolate MGMM8 warrants phenotypic assessment to gauge its potential in biocontrol and bioremediation. This evaluation extends to isolating active compounds for potential antimicrobial activities against pathogenic microorganisms. The comprehensive genome annotation process has facilitated the genetic characterization of MGMM8 and has solidified its potential as a biotechnological strain to address global anthropogenic predicaments.

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Section: Introductionmentioning

confidence: 99%

Biotechnological Key Genes of the Rhodococcus erythropolis MGMM8 Genome: Genes for Bioremediation, Antibiotics, Plant Protection, and Growth Stimulation

Afordoanyi,

Akosah,

Shnakhova

et al. 2023

Microorganisms

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“…[40][41][42][43][44][45], Rhodococcus sp. [46][47][48][49][50][51]. Among the fungi capable of metabolizing oil-derived substances are Aspergillus sp.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of the Effectiveness of Bioaugmentation-Assisted Phytoremediation of Soils Contaminated with Petroleum Hydrocarbons Using Echinacea purpurea

Wojtowicz,

Steliga,

Kapusta

2023

Applied Sciences

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Phytoremediation supported by bioaugmentation is a promising method considered for cleaning up areas polluted with petroleum hydrocarbons. In this study, phytoremediation was carried out using Echinacea purpurea as a phytoremediant on two types of soil: Soil DW—aged soil taken from an excavation pit, Soil OS—soil taken from an oil spill area. The tests for each soil were carried out in six test systems (non-inoculation, inoculation with the B1 microbial consortium, inoculation with the B2 microbial consortium, inoculation with the B1 microbial consortium with the addition of γ-PGA (γ-poly glutamic acid), inoculation with the B2 microbial consortium with the addition of γ-PGA and inoculation with the γ-PGA solution) for 6 months. The effectiveness of the remediation treatments used was assessed based on chromatographic analyses of soil and plant material (roots, shoots) and toxicological analyses using four types of toxicological tests (PhytotoxkitTM (MicroBioTests Inc., Gent, Belgium), OstracodtoxkitTM (MicroBioTests Inc., Gent, Belgium), Microtox® Solid Phase Test (Modern Water Inc., New Castle, DE, USA), MARA (NCIMB Ltd., Aberdeen, UK)). The research conducted showed that the most effective method of bioremediation of soils contaminated with petroleum hydrocarbons was phytoremediation supported by bioaugmentation with the microbial consortium B2 with γ-PGA, which allowed for reducing the concentration of total petroleum hydrocarbons (TPHs) and polycyclic aromatic hydrocarbons (PAHs) in the tested soils by 53.98% and 49.54% (Soil DW-5) and 60.47% and 37.55% (Soil OS-5), respectively. In turn, the lowest bioremediation efficiency was recorded in non-inoculated systems, for which the concentration of TPHs and PAHs at the end of the study decreased by 18.40% and 16.14% (Soil DW-1) and 21.87% and 18.20% (Soil OS-1), respectively. The results of toxicological analyses confirmed the relationship between the concentration of TPHs and PAHs in the soil and its toxicity level.

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Biodegradation of Environmental Pollutants by Autochthonous Microorganisms – A Precious Service for the Restoration of Impacted Ecosystems

Fernandes

Alexandrino

Mucha

et al. 2022

Assessing the Microbiological Health of Ecosystems

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Bioremediation of the Petroleum Contaminated Desert Steppe Soil with Rhodococcus erythropolis KB1 and Its Effect on the Bacterial Communities of the Soils

Cited by 7 publications

References 32 publications

Biotechnological Key Genes of the Rhodococcus erythropolis MGMM8 Genome: Genes for Bioremediation, Antibiotics, Plant Protection, and Growth Stimulation

Biotechnological Key Genes of the Rhodococcus erythropolis MGMM8 Genome: Genes for Bioremediation, Antibiotics, Plant Protection, and Growth Stimulation

Evaluation of the Effectiveness of Bioaugmentation-Assisted Phytoremediation of Soils Contaminated with Petroleum Hydrocarbons Using Echinacea purpurea

Biodegradation of Environmental Pollutants by Autochthonous Microorganisms – A Precious Service for the Restoration of Impacted Ecosystems

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