There was a significant regression in the inflammatory reaction in the capsules indicating, therefore, that Biodentine is a biocompatible material.
Biodentine and MTA exhibit potential as repair material in the treatment of furcation perforations.
Considering that endodontic sealers release some components which may promote delay in the repair process, our purpose was to evaluate the tissue reaction promoted by MTA Plus and MTA Fillapex in comparison with AH Plus (standard control) and Endofill, which has a long clinical track record. One hundred rats were distributed into five groups: MTA Plus (Avalon Biom Inc., Bradenton, FL, USA), MTA Fillapex (Angelus, Londrina, PR, Brazil), AH Plus (Dentsply DeTrey GmbH, Konstanz, Germany), Endofill (Dentsply, Petrópolis, RJ, Brazil) and CG (control group, empty polyethylene tubes). The polyethylene tubes filled with sealers or empty (CG) were implanted into subcutaneous. After 7, 15, 30 and 60 days, the tubes surrounded by capsules were paraffin-embedded. In HE-stained sections, the volume density of inflammatory cells (VvIC) was estimated in the capsules. The number of interleukin-6-immunolabelled cells (IL-6), a pro-inflammatory cytokine, was also computed in the capsules. The birefringent collagen content was quantified in picrosirius-stained sections. Data were analysed by ANOVA and Tukey tests (p ≤ 0.05). At 7 days, the capsules showed moderate inflammatory reaction. In all groups, VvIC and IL-6-immunostained cells reduced significantly from 7 to 60 days. At 60 days, IL-6 immunoexpression was reduced significantly in MTA Plus and MTA Fillapex in comparison with AH Plus; no difference was found in the VvIC among MTA Plus, MTA Fillapex, AH Plus and CG whereas Endofill exhibited the highest VvIC. The reduction in VvIC was parallel to an increase in the collagen in all the groups, except Endofill. MTA Plus, MTA Fillapex and AH Plus induce a response that culminates in the regression of inflammation and formation of a fibrous capsule over time. The lower IL-6 immunoexpression in the capsules of MTA Plus and MTA Fillapex than AH Plus suggests that the immune response is suppressed more rapidly in the MTA-based sealers.
The experimental materials (CS + ZrO and CS + Nb O ) induced fibroblast proliferation and accelerated the regression of the inflammatory reaction. However, the addition of nanoparticulate radiopacifiers did not improve the biological properties of a calcium silicate-based cement when compared to microparticulate agents.
The collagen-rich capsule formed slowly around Biodentine in comparison with MTA. FGF-1 and mast cells participated in capsule remodelling, stimulating fibroblast proliferation and subsequent collagen production, in response to subcutaneous implants.
Aim:The aim of the following study is to evaluate the cleaning capacity of a hybrid instrumentation technique using Reamer with Alternating Cutting Edges (RaCe) system files in the apical third of mesial roots of mandibular molars.Materials and Methods:Twenty teeth were selected and separated into two groups (n = 20) according to instrumentation technique as follows: BioRaCe - chemomechanical preparation with K-type files #10 and #15; and files BioRaCe BR0, BR1, BR2, BR3, and BR4; HybTec - hybrid instrumentation technique with K-type files #10 and #15 in the working length, #20 at 2 mm, #25 at 3 mm, cervical preparation with Largo burs #1 and #2; apical preparation with K-type files #15, #20, and #25 and RaCe files #25.04 and #30.04. The root canals were irrigated with 1 ml of 2.5% sodium hypochlorite at each change of instrument. The specimens were histologically processed and photographed under light optical microscope. The images were inserted onto an integration grid to count the amount of debris present in the root canal.Results:BioRaCe presented the highest percentage of debris in the apical third, however, with no statistically significant difference for HybTec (P > 0.05).Conclusions:The hybrid technique presented similar cleaning capacity as the technique recommended by the manufacturer.
O tratamento endodôntico permite a permanência de dentes na cavidade oral. No entanto, procedimentos inadequados podem ocasionar o escurecimento dentário, acometendo a estética do sorriso e levando à baixa autoestima do paciente. O objetivo do presente estudo é relatar um caso clínico de retratamento endodôntico não cirúrgico, seguido de clareamento endógeno em dentes anteriores escurecidos para reabilitar os elementos tanto funcional quanto esteticamente. Paciente do gênero feminino, 25 anos, compareceu à clínica de Odontologia de uma Universidade relatando insatisfação com seu sorriso. A queixa principal se fundamentava na baixa autoestima em decorrência do escurecimento dos incisivos central e lateral superiores esquerdos após tratamento endodôntico realizado oito meses antes. Ambos os dentes estavam assintomáticos, mas clinicamente exibiam exposição de guta-percha sem selamento coronário. Radiograficamente o elemento 21 apresentava sobre-extensão de material obturador. A conduta adotada foi a reintervenção endodôntica dos elementos 21 e 22 com sepultamento do fragmento de guta-percha extrarradicular e posterior clareamento dentário interno, além de restaurações em ambos os elementos. O retratamento endodôntico não cirúrgico e o clareamento endógeno foram eficazes para a resolução do caso, pois possibilitaram a manutenção dos elementos dentários em função na cavidade oral e o restabelecimento da estética do sorriso, garantindo satisfação da paciente.Descritores: Endodontia; Retratamento; Clareamento Dental.ReferênciasTorabinejad M, White SN. Endodontic treatment options after unsuccessful initial root canal treatment: alternatives to single-tooth implants. J Am Dent Assoc. 2016;147(3):214-20.Mautone EP, Oliveira EPM, Bonatto SVS, Melo TAF, Queiroz MLP. Desobturação e repreparo do canal radicular: análise da eficácia de duas técnicas preconizadas. Rev Odontol Bras Central. 2014;23(64):58-64.Mozardo DSB, Hussne RP, Nishiyama CK, Bodanezi A, Câmara AS. 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Arch Health Invest. 2017;6(5):213-17.Greenwall-Cohen J, Greenwall LH. The single discoloured tooth: vital and non-vital bleaching techniques. Br Dent J. 2019;226(11):839-49.Kaled GH, Faria MIA, Heck AR, Aragão EM, Morais SH, de Souza RC. Retratamento endodôntico: análise comparativa da efetividade da remoção da obturação dos canais radiculares realizada por três métodos. RGO Rev gauch odontol. 2011;59(1):103-8.Rocha MP, Silva RV, Silva LRM, Rocha TCM, Brito AM, Pereira RP. Retratamento endodôntico não cirúrgico: relato de caso. Rev Odontol Univ Cid São Paulo. 2016;28(3):270-76.Chandrasekar C, Ebenezer AVR, Kumar M, Sivakumar A. A comparative evaluation of gutta percha removal and extrusion of apical debris by rotary and hand files. J Clin Diagn Res. 2014;8(11):ZC110-14.Kasam S, Mariswamy AB. Efficacy of different methods for removing root canal filling material in retreatment: an in-vitro study. J Clin Diagn Res. 2016;10(6):ZC6-10.Ozyurek T, Tek V, Yilmaz K, Uslu G. Incidence of apical crack formation and propagation during removal of root canal filling materials with different engine driven nickel- titanium instruments. Restor Dent Endod. 2017;42(4):332-41.Rubino GA, Candeiro GTM, Freire LG, Iglecias EF, Lemos EM, Caldeira Cl, et al. Micro-CT evalution of guta-percha removal by two retreatment systems. Iran Endod J. 2018; 13(2):221-27.Kesim B, Ustun Y, Aslan T, Topçuoglu HS, Sahin S, Ulusan O. Efficacy of manual and mechanical instrumentation techniques for removal of overextended root canal filling material. Niger J Clin Pract. 2017;20(6):761-66.Lucena MTL, Mantovani M, Fracalossi C, Silva GR. Clareamento interno em dentes desvitalizados com a técnica walking bleach: relato de caso. Uningá Review. 2015;24(1):33-9.Ricucci D, Russo J, Rutberg M, Burleson JA, Spangberg LSW. A prospective cohort study of endodontic treatments of 1,369 root canals: results after 5 years. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2011;112(6):825-42.Gomes AC, Nejaim Y, Silva AIV, Neto FH, Cobenca N, Zaia AA et al. Influence of endodontic treatment and coronal restoration on status of periapical tissues: a cone-beam computed tomographic study. J Endod. 2015;41(10):1614-18.Plotino G, Buono L, Grande NM, Pameijer CH, Somma F. Nonvital tooth bleaching: a review of the literature and clinical procedures. J Endod. 2008;34(4):394-407.Abbott P, Heah SYS. Internal bleaching of teeth: an analysis of 255 teeth. Aust Dent J. 2009; 54(4):326-33.Badole GP, Warhadpande MM, Bahadure RN, Badole SG. Aesthetic rehabilitation of discoloured nonvital anterior tooth with carbamide peroxide bleaching: case series. J Clin Diagn Res. 2013; 7(12):3073-76.Madhu KS, Hegde S, Mathew S, Lata DA, Bhandi SH, Shruthi N. Comparison of radicular peroxide leakage from four commonly used bleaching agents following intracoronal bleaching in endodontically treated teeth: an in vitro study. 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Anatomia e Histologia são a base das Ciências da Saúde, como a Odontologia, pois estudam estruturas e funções do organismo em um contexto macroscópico e microscópico, respectivamente, constituindo as Ciências Morfológicas. O objetivo deste estudo é compreender e avaliar o processo de ensino-aprendizagem de Anatomia e Histologia por estudantes do curso de Odontologia. O método adotado foi a revisão da literatura relativa à importância do ensino de Anatomia e Histologia em Odontologia, além de metodologias ativas de aprendizagem aplicáveis ao processo de ensino-aprendizado destas disciplinas. Realizou-se buscas nas bases de dados Pubmed, Scielo, Lilacs e Google Acadêmico, utilizando-se as palavras-chave “odontologia”, “histologia”, “anatomia” e “ensino”, em português e inglês e sem limite temporal de publicação. Os dados foram interpretados qualitativamente. O ensino-aprendizagem deve ser elaborado segundo os domínios cognitivo, psicomotor e afetivo, em consonância com os objetivos educacionais do curso de graduação. Aprendizagem Baseada em Problema é uma metodologia ativa que promove compreensão adequada e verdadeiramente significativa pela resolução de situações-problema inerentes à profissão. Dispositivos eletrônicos auxiliam no desenvolvimento efetivo de ambas as disciplinas. Conclui-se que metodologias ativas de aprendizagem podem promover maior aprendizado das Ciências Morfológicas, sendo primordial para a futura adequada atuação em Odontologia.
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