Zirconium oxide and niobium oxide used as radiopacifiers in a calcium silicate‐based material stimulate fibroblast proliferation and collagen formation

Silva, G. F.; Guerreiro-Tanomaru, Juliane Maria; Fonseca, Tiago Silva da; Bernardi, M. I. B.; Sasso‐Cerri, Estela; Tanomaru-Filho, Mário; Cerri, Paulo Sérgio

doi:10.1111/iej.12789

Cited by 42 publications

(52 citation statements)

References 47 publications

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“…, Silva et al . ). In the present study, a concomitant presence of FGF‐1‐positive cells and Ki‐67‐labelled cells was observed in the capsules of both calcium silicate materials and in the control group, reinforcing the concept that FGF‐1 stimulates mitotic activity (Zakrzewska et al .…”

Section: Discussionmentioning

confidence: 97%

“…, Silva et al . ). The greatest immunoexpression for FGF‐1 accompanied by accentuated Ki‐67‐immunolabelled cells found at 7 days indicates that FGF‐1 may have stimulated fibroblast proliferation, explaining the significant and gradual increase of these cells in all groups.…”

Section: Discussionmentioning

confidence: 97%

“…In addition, the significant increase in the collagen content in the capsules over time indicates that FGF‐1 may also stimulate fibroblast activity (Silva et al . ).…”

Section: Discussionmentioning

confidence: 97%

“…The morphological evaluation and fibroblast number in the capsules were performed from sections stained with Masson's trichrome (Silva et al . ). For each implant, three non‐serial sections, at intervals of at least 100 μm, were used.…”

Section: Methodsmentioning

confidence: 97%

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Mast cells and immunoexpression of FGF‐1 and Ki‐67 in rat subcutaneous tissue following the implantation of Biodentine and MTA Angelus

et al. 2018

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Section: Discussionmentioning

confidence: 97%

Section: Discussionmentioning

confidence: 97%

“…In addition, the significant increase in the collagen content in the capsules over time indicates that FGF‐1 may also stimulate fibroblast activity (Silva et al . ).…”

Section: Discussionmentioning

confidence: 97%

Section: Methodsmentioning

confidence: 97%

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Mast cells and immunoexpression of FGF‐1 and Ki‐67 in rat subcutaneous tissue following the implantation of Biodentine and MTA Angelus

et al. 2018

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“…The coordinate action of these chemokines regulates the degree of the inflammatory reaction as well as its regression and tissue repair 5 . A biocompatible material may allow the release of mediators by host cells which promote the regression of the inflammatory reaction 4,18,[20][21][22] and stimulate the tissue repair 5,22 . Among the cytokines and growth factors, the interleukin-6 (IL-6) has a participation in the initiation and maintenance of the inflammatory reaction 4,5,[23][24][25] .…”

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confidence: 99%

Immunoinflammatory response and bioactive potential of GuttaFlow bioseal and MTA Fillapex in the rat subcutaneous tissue

Delfino

Guerreiro-Tanomaru

Tanomaru‐Filho

et al. 2020

Sci Rep

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To evaluate the effect of GuttaFlow bioseal (GFB) and MTA Fillapex (MTAF) in comparison with Endofill (EF) in the subcutaneous tissue. Polyethylene tubes with GFB, MTAF, EF or empty tubes (control group; CG) were implanted into subcutaneous of rats. After 7, 15, 30 and 60 days, the capsule thickness, inflammatory reaction, interleukin-6 (IL-6), vascular endothelial growth factor (VEGF), caspase-3, TUNEL-positive cells, von Kossa and ultrastructural features were evaluated. The data were statistically analyzed (p ≤ 0.05). At all periods, the number of IL-6-and VEGF-immunolabelled cells, and capsule thickness were lower in GFB than MTAF, which was lower than EF (p < 0.0001). At 60 days, the number of inflammatory cells was similar in GFB and MTAF (p = 0.58). Significant differences in the number of TUNEL-and caspase-3-positive cells were not observed among GFB, MTAF and CG whereas the highest values were found in EF specimens. The EF specimens exhibited several cells with condensed chromatin, typical of apoptosis. von Kossa-positive and birefringent structures were only observed in GFB and MTAF, suggesting the presence of calcite crystals. Taken together, these results show that cellular and structural damage induced by GFB and MTAF sealers were recovery over time. Moreover, these sealers express bioactive potential in subcutaneous tissue.The clinical use indication of root canal sealers depends on their physicochemical and biological properties. Thus, the biological properties of endodontic sealers and degree of cytotoxicity on different cells, including fibroblasts, osteoblasts and cementoblasts have been widely investigated 1 . However, in vitro studies have the disadvantage of reflecting the response to a specific cell type and, therefore, do not reflect the host response, which is under influence of several cytokines and growth factors 2 . The implant into subcutaneous connective tissue is widely used to evaluate the biocompatibility and the complex cascade of cellular and molecular events induced by dental materials 2-5 .Studies have suggested that silicone-based endodontic sealers exhibit proper physicochemical and biological properties 6,7 . A novel formulation of silicone-based sealer, the GuttaFlow bioseal (GFB; Coltene Whaledent, GmBH + Co KG, Langenau, Switzerland) is a mixture of gutta-percha powder and polydimethylsiloxane with silver nanoparticles added as a preservative; silica, calcium oxide and phosphorous oxide particles were also combined to this mixture to provide bioactivity and stimulate the tissue repair. The GFB has low solubility and porosity, alkalinizing activity and slight calcium release 8,9 . An in vitro study using human periodontal ligament cells has demonstrated that GFB displays better cytocompatibility than AH Plus 10 , which is considered as a gold standard in the clinical endodontic.Considering the suitable biocompatibility of MTA (Mineral Trioxide Aggregate, Angelus, Londrina, PR, Brazil), the manufactures have added tricalcium silicate to resin-based sealers in an attempt to ...

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Higher hydration performance and bioactive response of the new endodontic bioactive cement MTA HP repair compared with ProRoot MTA white and NeoMTA plus

2019

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The aim of this study was to characterize the hydration performance and the bioactive response of the new bioactive endodontic cement MTA HP repair (HP), comparing its physicochemical parameters with those of ProRoot MTA White (Pro) and NeoMTA Plus (Neo). Un-hydrated precursor materials were characterized by X-ray fluorescence, laser diffraction, N 2 physisorption and field emission gun scanning electron microscopy (FEG-SEM). Setting time was assessed according to ASTM specification C 266. Hydrated materials were analyzed by X-ray diffraction, Fourier transform infrared spectroscopy (FT-IR) and (FEG-SEM). Bioactivity evaluation in vitro was carried out, by soaking processed cement disk in simulated body fluid (SBF) during 168 h. The cements surface was studied by FT-IR, FEG-SEM, and energy dispersive X-ray. Release to the SBF media of ionic degradation products was monitored using inductively coupled plasma atomic emission spectroscopy. HP showed shorter initial setting time compared to Pro and Neo and produce a quick and effective bioactive response in vitro in terms of phosphate phase surface coating formation. This higher bioactive response for HP is correlated with increasing calcium aluminate content, increasing surface area of un-hydrated powder precursor and the increasing release capacity of Si ionic products of the final hydrated product. The higher bioactive response of MTA HP repair highlights this material, as very interesting to further investigate its performance to improve the outcome of vital pulp therapy procedures.

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Zirconium oxide and niobium oxide used as radiopacifiers in a calcium silicate‐based material stimulate fibroblast proliferation and collagen formation

Cited by 42 publications

References 47 publications

Mast cells and immunoexpression of FGF‐1 and Ki‐67 in rat subcutaneous tissue following the implantation of Biodentine and MTA Angelus

Mast cells and immunoexpression of FGF‐1 and Ki‐67 in rat subcutaneous tissue following the implantation of Biodentine and MTA Angelus

Immunoinflammatory response and bioactive potential of GuttaFlow bioseal and MTA Fillapex in the rat subcutaneous tissue

Higher hydration performance and bioactive response of the new endodontic bioactive cement MTA HP repair compared with ProRoot MTA white and NeoMTA plus

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