Reduced interleukin-6 immunoexpression and birefringent collagen formation indicate that MTA Plus and MTA Fillapex are biocompatible

Abstract

Considering that endodontic sealers release some components which may promote delay in the repair process, our purpose was to evaluate the tissue reaction promoted by MTA Plus and MTA Fillapex in comparison with AH Plus (standard control) and Endofill, which has a long clinical track record. One hundred rats were distributed into five groups: MTA Plus (Avalon Biom Inc., Bradenton, FL, USA), MTA Fillapex (Angelus, Londrina, PR, Brazil), AH Plus (Dentsply DeTrey GmbH, Konstanz, Germany), Endofill (Dentsply, Petrópolis, RJ, Brazil) and CG (control group, empty polyethylene tubes). The polyethylene tubes filled with sealers or empty (CG) were implanted into subcutaneous. After 7, 15, 30 and 60 days, the tubes surrounded by capsules were paraffin-embedded. In HE-stained sections, the volume density of inflammatory cells (VvIC) was estimated in the capsules. The number of interleukin-6-immunolabelled cells (IL-6), a pro-inflammatory cytokine, was also computed in the capsules. The birefringent collagen content was quantified in picrosirius-stained sections. Data were analysed by ANOVA and Tukey tests (p ≤ 0.05). At 7 days, the capsules showed moderate inflammatory reaction. In all groups, VvIC and IL-6-immunostained cells reduced significantly from 7 to 60 days. At 60 days, IL-6 immunoexpression was reduced significantly in MTA Plus and MTA Fillapex in comparison with AH Plus; no difference was found in the VvIC among MTA Plus, MTA Fillapex, AH Plus and CG whereas Endofill exhibited the highest VvIC. The reduction in VvIC was parallel to an increase in the collagen in all the groups, except Endofill. MTA Plus, MTA Fillapex and AH Plus induce a response that culminates in the regression of inflammation and formation of a fibrous capsule over time. The lower IL-6 immunoexpression in the capsules of MTA Plus and MTA Fillapex than AH Plus suggests that the immune response is suppressed more rapidly in the MTA-based sealers.

Mast cells and immunoexpression of FGF‐1 and Ki‐67 in rat subcutaneous tissue following the implantation of Biodentine and MTA Angelus

“…The increase in collagen content within the capsules accompanied by evident reduction in the inflammatory reaction indicate that Biodentine and MTA Angelus may induce a response that culminates in tissue repair (Saraiva et al . ) and, therefore, these materials are biocompatible.…”

“…, Saraiva et al . ). However, cellular and molecular mechanisms involved in replacing the inflammatory reaction by healthy connective tissue, as observed in the repair process, are still poorly understood.…”

Immunoinflammatory response and bioactive potential of GuttaFlow bioseal and MTA Fillapex in the rat subcutaneous tissue

“…The endodontic sealers interfere on the outcome of the endodontic therapy, since the host cells are responsible for production of several growth factors and cytokines. The coordinate action of these chemokines regulates the degree of the inflammatory reaction as well as its regression and tissue repair 5 . A biocompatible material may allow the release of mediators by host cells which promote the regression of the inflammatory reaction 4,18,[20][21][22] and stimulate the tissue repair 5,22 .…”

“…The coordinate action of these chemokines regulates the degree of the inflammatory reaction as well as its regression and tissue repair 5 . A biocompatible material may allow the release of mediators by host cells which promote the regression of the inflammatory reaction 4,18,[20][21][22] and stimulate the tissue repair 5,22 . Among the cytokines and growth factors, the interleukin-6 (IL-6) has a participation in the initiation and maintenance of the inflammatory reaction 4,5,[23][24][25] .…”

“…A biocompatible material may allow the release of mediators by host cells which promote the regression of the inflammatory reaction 4,18,[20][21][22] and stimulate the tissue repair 5,22 . Among the cytokines and growth factors, the interleukin-6 (IL-6) has a participation in the initiation and maintenance of the inflammatory reaction 4,5,[23][24][25] . Other chemokine involved in the inflammatory reaction is the vascular endothelial growth factor (VEGF), an angiogenic factor that increases the vascular permeability 26 favouring the recruitment of inflammatory cells in the beginning of the inflammatory reaction 27 , and plays an essential role in the tissue remodelling 28 and wound healing 27 .…”