ABSTRAK Penelitian ini dilakukan untuk mengetahui efektifitas pengencer Zorlesco dan modifikasi Zorlesco dengan beberapa jenis susu kacang kedelai dalam mempertahankan fertilitas spermatozoa babi landrace yang disimpan pada suhu 18⁰C. Semen dikoleksi dua kali seminggu menggunakan metode masase dari dua ekor pejantan landrace berumur ±2,5 tahun yang memiliki kodisi tubuh dan organ reproduksi yang sehat. Semen yang memenuhi syarat setelah di evaluasi secara makroskopis dan mikroskopis diencerkan dengan Zorlesco (P0) atau modifikasi Zorlesco dengan suplementasi susu kacang kedelai murni (P1), Metabolis (P2) dan Melilea (P3), dan masing-masing perlakuan diulang lima kali. Semen selanjutnya disimpan pada suhu 18⁰C. Data tentang motilitas dan viabilitas spermatozoa di analisis dengan ANOVA dan dilanjutkan dengan uji Duncan. Hasil analisis statistik menunjukkan bahwa modifikasi pengencer Zorlesco yang ditambahkan dengan susu kedelai 5% tidak dapat (P<0.05) mempertahankan motilitas dan viabilitas spermatozoa lebih tinggi dari pengencer Zorlesco selama 42 jam penyimpanan. Kata Kunci: susu kacang kedelai, modifikasi Zorlesco, motilitas Spermatozoa, viabilitas spermatozoa, babi landrace Sperm Fertility of Landrace Boar in Modified Zorlesco Extender with Soymilk ABSTRACT A research has been done to study the effectiveness of Zorlesco extender and modified Zorlesco extender with soymilk to maintain sperm fertility of landrace boar preserved at 18˚C. Semen was collected twice a week by hand massage from 2 heads of ± 2.5 years old landrace boar. A Completely Randomized Design with 4 treatments and 5 replications was used to organized the experiment. The fresh evaluated semen were extended with zorlesco P(0), zorlesco + hand made soymilk P(1), zorlesco + Metabolis TM soymilk P(2), and zorlesco + Melilea TM soymilk P(3). Extended semen were preserved at 18 0 C. Analysis of variance and Duncan test were used to analyzed data of motility and viability of sperm. The result showed that the modification of diluent Zorlesco added with 5% soymilk could not defend by sperm motility and viability spermatozoa compared with Zorlesco for 42 hours storage (P<0.05).
An experiment had been conducted in Merbaun Village, Regency of Kupang for three months. The objective of the experiment was to know characteristics of Bali cattle Bull i.e. age and body weight, body score condition, linier body size (e.g. chest girth, body length, and shoulder height), libido, scrotum circumference, and semen production. 125 of Bali cattle Bull with aged about 2-2,5 years were used in physical measured while nine Bull were choosen randomly to measure libido and semen production. The results of the experiment showed that the average age and body weight were 25.83 ± 0.3719 months and 207.37 ± 1.84 kg, with BCS of 3.97 ± 0.04 (from 5 score), and the chest girth, body length, and shoulder height were 158.27 ± 0.71 cm, 106.87 ± 0.26 cm, and 108.55 ± 0.37 cm, respectively. About 77 head (61.6%) was brown colour, and the other 48 (38.4%) was black, with moderate of capacity of libido (score 7.22 ± 0.66 from 9 score). The average of scrotum circumference was 25.67±1.20 cm, volume of semen were 2.54±0.69 ml and sperm concentration was 1.26 x 109 per ml repectively. Of the total of 125 Bali cattle young bull, only 26% fulfilled the Indonesia Standard of Bali young bull.Key word : young bull, bali cattel, traditionally livestock.
This study aimed to determine the effect of palmyra juice and sugarcane juice supplementation in durasperm diluent on the quality of Duroc boar liquid semen. The source of semen a from 3 Duroc boar aged 2-3 years, which were collected twice a week using the glove hand method. Semen was evaluated macroscopically and microscopically, good quality semen was divided into three parts and subjected to treatment: durasperm (PO), P1 durasperm + palmyra juice (PJ), P2 durasperm + sugarcane juice (SJ) and then stored in a Styrofoam box at 18 o -20 o C. Observations were made every eight hours. The data obtained were analyzed using Analysis of Variance (ANOVA) using the SPSS 20 program. The results showed that the percentage of motility at 64 hours showed that P1 was 40% higher (P <0.05) than P2 (35.00 ± 1.82) and P0 (27.50 ± 2.88). P1 had a higher percentage of spermatozoa viability 50.94 ± 0.79 (P <0.05) than P2 (45.50 ± 1.94) and P0 (37.75 ± 3.48). P1 has a higher MPU of 52.92 ± 0.61 with P2 (47.38 ± 1.47) and P0 (40.49 ± 3.47). Abnormality and pH were not significantly different (P˃0.05). It was concluded that palmyra juice supplementation diluent (P1) was effective in maintaining motility, viability, abnormalities, MPU and maintaining a stable pH.
The objective of this study was to test banana stem silage as a substitute for corn on the performance and protein utilization rate of Duroc crosses pigs in the rearing phase. A total of 12 Duroc crosses pigs in the rearing phase with an average initial weight of 51.25 kg (KV = 7.02%). A completely randomized design (CRD) with 4 treatments and 3 replicates was used in this study. The treatments were T0: ration without a mixture of fermented banana stems (FBS), T1: ration with 5% FBS instead of cornmeal, T2: ration with 10% FBS instead of cornmeal, and T3: ration with 15% FBS instead of cornmeal. The variables measured were ration consumption, daily weight gain, ration utilization and protein utilization efficiency. The results showed that the use of FBS up to 15% (instead of 40% corn) had no effect on ration consumption, daily weight gain, ration utilization and protein utilization efficiency. Therefore, it can be concluded that banana stems can be used at 15% in the ration of Duroc crosses pigs in the rearing phase as a substitute for corn meal.
The goal of the first research was to find the best level of the used of the fructose into citrate of egg yolk (S-KT) on the presentage of the motility, viability, abnormality and the whole of the plasma membrane liquid semen of pig Landrace kept in 18 o C degree and to be evaluation in six (6) hours. The research design for this stage include five treatments and four (4) replications. They were citrate of egg yolk (S-KT) without fructose (P0), S-KT + fructose 2.5% (P1), S-KT + fructose 5% (P2), S-KT + fructose 7.5% (P3) and S-KT + fructose 10% (P4). The result of the research show the quality of the fresh semen is better because the motility and viability for each spermatozoid is 83.75±4.79% and 92.70±0.14%. In vitro stage for 24 hours into the S-KT liquid that suplement by fructose 5% (P2) showed the motility (40.00±4.08%) and the viability (54.10±4.35%) higher so there was a significant different (P<0.05) compared with another liquid material. The abnormality and the whole of plasma membrane spermatozoid to the fourth liquid didn't have any different (P>0.05). The second research showed the score presentage of pregnant (for animal) and S/C. The highest score percentage of pregnant in this research was P1 with the score was 80%, the lowest in P0 and P3 was 60%, and the lowest score S/C was P1 with the score was 1.25% and the higher in P0 and P2 was 1.67%. The conclusion for the research is the fruktose suplement 5% in citrate of egg yolk can be hold out of the motility and viability of spermatozoid for pig Landrace up to 40% during 24 hours observation in the pregnant score is 67.50%. Key words:Pig semen, citrate of egg yolk, motility, unnatural insemination, pregnant ABSTRAKTujuan penelitian tahap I adalah untuk menentukan level terbaik penggunaan fruktosa dalam pengencer SitratKuning Telur (S-KT) terhadap persentase motilitas, viabilitas, abnormalitas serta keutuhan membran plasma semen cair babi Landrace yang disimpan pada suhu 18 o C dan dievaluasi setiap 6 jam. Rancangan penelitian pada tahap ini terdiri dari 5 perlakuan dan 4 ulangan yaitu sitrat-kuning telur (S-KT) tanpa Fruktosa (P0), S-KT + Fruktosa 2,5% (P1), S-KT + Fruktosa 5% (P2), S-KT + Fruktosa 7,5% (P3) dan S-KT + Fruktosa 10% (P4). Hasil penelitian menunjukkan bahwa kualitas semen segar cukup baik karena motilitas dan viabilitas spermatozoa masing-masing adalah 83,75±4,79% dan 92,70±0,14%. Tahap in vitro selama 24 jam dalam pengencer S-KT yan g suplementasi fruktosa 5% (P2) menunjukkan motilitas (40,00±4,08%) dan viabilitas (54,10±4,35%) lebih tinggi sehingga menunjukan perbedaan nyata (P<0,05) dibandingkan dengan bahan pengencer lainnya. Abnormalitas dan keutuhan membran plasma spermatozoa dalam keempat pengencer tidak berbeda (P>0,05). Penelitian tahap II untuk mengetahui persentase angka kebuntingan dan S/C. Persentase tertinggi angka kebuntingan dalam penelitian ini adalah P1 dengan nilai 80%, terendah pada P0 dan P3 yaitu 60%. Sedangkan nilai S/C terendah adalah P1 dengan nilai 1,25% dan tertinggi pada P0 dan P3 yaitu 1,67%. Kesim...
The present study used the ratio of 80% coconut water and 11 - 20% egg yolk substitution with extract guava ranged from 4 to 9% in order to determine the quality and fertility of Bali cattle storaged at 50C. This study used a complete factorial design with two factors and six treatments. The first factor was coconut water and egg yolk substituted with extract guava extender at different ratio consisted of 6 treatments. They were: (1) T0 (control) = 80% coconut water + 20% egg yolk without extract guava; (2) T1 = 80% coconut water + 16% egg yolk + 4% extract guava; (3) T1 = 80% coconut water + 15% egg yolk + 5% extract guava; (4) T2 = 80% coconut water + 14% egg yolk + 6% extract guava; (5) T3 = 80% coconut water + 13% egg yolk + 7%; and (5) T4 = 80% coconut water + 12% egg yolk + 8% extract guava; and (6) T5 = 80% coconut water + 11% egg yolk + 9% extract guava. The second factor was the length of storage at low temperature which consisted of 3 treatments, including 1 day, 2 days, 3 days, 4 days, 5 days, 6 days and 7 days. The semen used in this study had motility ranged from 50 to 55%. The variables measured in this study were intact plasma membrane (IPM) and intact acrosome hood (ICH). The results showed that the percentage of spermatozoa motility for T1 until the sixth day of storage was 55.51%, T2 until the seventh day of storage was 40.14%, T0 to the fifth day was 41.73%, T3 and T4 until the fourth day was 43.85% and 41.11%, respectively, whereas for T5 only until the third day were 51.00% and T6 until the second day amounted to 48.83%. Viability of Bali cattle spermatozoa in the six treatment groups where egg yolk substituted with extract guava was higher (T1, T2 and T6) compared to T0 from the first day until the fifth day of storage. It can be concluded that substitution of 15% egg yolk with 5% extract guava in 80% coconut water diluents can maintain IPM and ICH spermatozoa of Bali cattle until the sixth day of storaged at 5°C.
This study aims to determine the effect of adding various doses of carrot juice into citrate-egg yolk diluents (C-EY) on bligon buck semen quality. Semen was collected twice a week using an artificial vaginal method of three buck with normal body condition and reproductive organs. Semen was 70% of motility, konsentration ≥1000x106mL and abnormality ≤ 15 diluted with C-EY added with carrot juice at a concentrations: 0% (CJ-0), 10% (CJ-10), 12.5% (CJ-12,5), 15% (CJ-15), 17.5% (CJ-17,5), and 20% (CJ-20), and stored in a refrigerator at 3-5o C. The quality of spermatozoa was evaluated every 24 hours. The Variables measured were spermatozoa motility, viability, and abnormalities. The results showed that spermatozoa preserved by C-EY supplemented by 17.5% carrot juice (CJ-17.5) had a higher quality (P<0.05) compared to the other four treatments, with motility (49.04 ± 1.0%), viability (55.70 ± 1.22%), dan abnormalities (4.57 ± 0.88 %) on the 4th day of storage. This study concludes that 17.5% carrot juice into citrate eggyolk extender improves the bligon buck semen
This study aimed to evaluate study the influence of cattle hypophysis extract (HE) on the reproductive performance of post partum sows. Twenty landrace sows, 45 days after partum were divided into four groups, five sows each. All were injected with HE intramuscularly. Group I (P0) was injected with 3 ml of saline solution, Group II (P1) was injected with 3 ml contain of 10 mg/EH/Kg body weight (BW), Group III (P2) was injected with 3 ml contain of 20 mg/EH/Kg BW, Group IV (P3) was injected with 3 ml contain of 40 mg/EH/Kg BW. Every sows that showed the estrus symptom were inseminated artificially on the second days, twice each in the morning and evening. The intensity of estrus, length of estrus, number of service preconception (S/C), conception rate (CR), litter size, weight of piglet and milk production were recorded. The result showed that the score of estrus intensity in Group I, II, III and IV were 1.40; 2.40; 2.80 and 2.80 respectively with the length of estrus in each group were 4.40; 5.00; 5.00; 5.60 days respectively. The number of service in Group I, II, III and IV of each pregnancy were 4.40; 1.40; 1.00 and 1.20 with the CR were 0; 60; 100; and 80% respectively. The litter size produced of Group I, II, III and IV were 4.00; 6.00; 10.60; 8.40 respectively. The average of piglet body weight of Group I, II, III and IV were 1.20; 1.36; 1.56 and 1.35 kg respectively. The milk production of each sows of Group I, II, III and IV were 13; 41; 93; 88 g respectively. It can be concluded that cattle hypophysis extract improves the performance of post partum sows with the best level was 20 mg EH/kg of body weight. ABSTRAK Penelitian ini bertujuan mempelajari pengaruh pemberian ekstrak hipofisis terhadap performans reproduksi induk babi betina post partum. Penelitian ini menggunakan 20 ekor induk babi betina jenis landrace, dibagi kedalam empat kelompok perlakuan masing-masing terdiri atas lima ekor induk babi. Babi betina yang baru 45 hari menyapih anaknya diinjeksi dengan ekstrak hipofisis (EH) secara intramuskuler. Kelompok I (P0) diinjeksi dengan 3 mL NaCl fisiologis; Kelompok II (P1) diinjeksi dengan 3 mL NaCL mengandung 10 mg EH/kg bobot badan (BB); Kelompok III (P2) diinjeksi dengan 3 mL NaCl mengandung 20 mg EH/kg BB: Kelompok IV (P3) diinjeksi dengan 3 mL NaCl mengandung 20 mg EH/kg BB. Setiap induk babi yang menunjukkan gejala estrus diinseminasi pada hari kedua dengan interval dua kali, pagi dan sore. Peubah yang diamati adalah intensitas estrus, lama estrus, jumlah perkawinan per kebuntingan (S/C), angka konsepsi (conception rate/CR), litter size, bobot lahir anak, dan produksi susu. Hasil penelitian menunjukkan skor intensitas estrus kelompok I, II, III, dan IV masing-masing 1,40; 2,40; 2,80; 2,80 dengan lama estrus masing-masing kelompok secara berturut-turut 4,40; 5,00; 5,00; 5,60 hari. Nilai S/C masing-masing adalah 4,40; 1,40; 1,00; 1,20 dengan CR masing-masing 0; 60; 100; dan 80%. Total jumlah anak yang dihasilkan kelompok I, II, III, dan IV masing-masing adalah 4,00; 6,00; 10,60; dan 8,40 ekor. Rataan bobot lahir anak per ekor pada kelompok I; II; III; dan IV masing-masing adalah 1,20; 1,36; 1,56; dan 1,35 kg. Produksi susu per induk pada kelompok I, II, III, dan IV masingmasing adalah 13; 41; 93, dan 88 g. Simpulan dari penelitian ini adalah ekstrak hipofisis dapat meningkatkan performans induk babi post partum. Dosis terbaik adalah 20 mg EH/kg BB.
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