Fertilitas In Vitro dan In Vivo Spermatozoa Babi Landrace pada Pengencer Sitrat Kuning Telur yang Disuplementasi Berbagai Level Fruktosa pada Penyimpanan Suhu 180C

Abstract

The goal of the first research was to find the best level of the used of the fructose into citrate of egg yolk (S-KT) on the presentage of the motility, viability, abnormality and the whole of the plasma membrane liquid semen of pig Landrace kept in 18 o C degree and to be evaluation in six (6) hours. The research design for this stage include five treatments and four (4) replications. They were citrate of egg yolk (S-KT) without fructose (P0), S-KT + fructose 2.5% (P1), S-KT + fructose 5% (P2), S-KT + fructose 7.5% (P3) and S-KT + fructose 10% (P4). The result of the research show the quality of the fresh semen is better because the motility and viability for each spermatozoid is 83.75±4.79% and 92.70±0.14%. In vitro stage for 24 hours into the S-KT liquid that suplement by fructose 5% (P2) showed the motility (40.00±4.08%) and the viability (54.10±4.35%) higher so there was a significant different (P<0.05) compared with another liquid material. The abnormality and the whole of plasma membrane spermatozoid to the fourth liquid didn't have any different (P>0.05). The second research showed the score presentage of pregnant (for animal) and S/C. The highest score percentage of pregnant in this research was P1 with the score was 80%, the lowest in P0 and P3 was 60%, and the lowest score S/C was P1 with the score was 1.25% and the higher in P0 and P2 was 1.67%. The conclusion for the research is the fruktose suplement 5% in citrate of egg yolk can be hold out of the motility and viability of spermatozoid for pig Landrace up to 40% during 24 hours observation in the pregnant score is 67.50%. Key words:Pig semen, citrate of egg yolk, motility, unnatural insemination, pregnant ABSTRAKTujuan penelitian tahap I adalah untuk menentukan level terbaik penggunaan fruktosa dalam pengencer SitratKuning Telur (S-KT) terhadap persentase motilitas, viabilitas, abnormalitas serta keutuhan membran plasma semen cair babi Landrace yang disimpan pada suhu 18 o C dan dievaluasi setiap 6 jam. Rancangan penelitian pada tahap ini terdiri dari 5 perlakuan dan 4 ulangan yaitu sitrat-kuning telur (S-KT) tanpa Fruktosa (P0), S-KT + Fruktosa 2,5% (P1), S-KT + Fruktosa 5% (P2), S-KT + Fruktosa 7,5% (P3) dan S-KT + Fruktosa 10% (P4). Hasil penelitian menunjukkan bahwa kualitas semen segar cukup baik karena motilitas dan viabilitas spermatozoa masing-masing adalah 83,75±4,79% dan 92,70±0,14%. Tahap in vitro selama 24 jam dalam pengencer S-KT yan g suplementasi fruktosa 5% (P2) menunjukkan motilitas (40,00±4,08%) dan viabilitas (54,10±4,35%) lebih tinggi sehingga menunjukan perbedaan nyata (P<0,05) dibandingkan dengan bahan pengencer lainnya. Abnormalitas dan keutuhan membran plasma spermatozoa dalam keempat pengencer tidak berbeda (P>0,05). Penelitian tahap II untuk mengetahui persentase angka kebuntingan dan S/C. Persentase tertinggi angka kebuntingan dalam penelitian ini adalah P1 dengan nilai 80%, terendah pada P0 dan P3 yaitu 60%. Sedangkan nilai S/C terendah adalah P1 dengan nilai 1,25% dan tertinggi pada P0 dan P3 yaitu 1,67%. Kesim...

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