Kualitas Semen Cair Babi Duroc dalam Pengencer Durasperm yang Disuplementasi Air Buah Lontar dan Sari Tebu

Abstract

This study aimed to determine the effect of palmyra juice and sugarcane juice supplementation in durasperm diluent on the quality of Duroc boar liquid semen. The source of semen a from 3 Duroc boar aged 2-3 years, which were collected twice a week using the glove hand method. Semen was evaluated macroscopically and microscopically, good quality semen was divided into three parts and subjected to treatment: durasperm (PO), P1 durasperm + palmyra juice (PJ), P2 durasperm + sugarcane juice (SJ) and then stored in a Styrofoam box at 18 o -20 o C. Observations were made every eight hours. The data obtained were analyzed using Analysis of Variance (ANOVA) using the SPSS 20 program. The results showed that the percentage of motility at 64 hours showed that P1 was 40% higher (P <0.05) than P2 (35.00 ± 1.82) and P0 (27.50 ± 2.88). P1 had a higher percentage of spermatozoa viability 50.94 ± 0.79 (P <0.05) than P2 (45.50 ± 1.94) and P0 (37.75 ± 3.48). P1 has a higher MPU of 52.92 ± 0.61 with P2 (47.38 ± 1.47) and P0 (40.49 ± 3.47). Abnormality and pH were not significantly different (P˃0.05). It was concluded that palmyra juice supplementation diluent (P1) was effective in maintaining motility, viability, abnormalities, MPU and maintaining a stable pH.

Determination of Alternative Diluents for Preservation of Duroc Boar Semen Through Modification of Various Semen Diluents

“…BTS and durasperm are two commonly used diluents for porcine sperm. Previous studies have shown that both diluents are able to maintain the quality of in vitro preserved porcine sperm, but only for a short period of time ( 17); (6).…”

PENGARUH PERBEDAAN WAKTU EKUILIBRASI TERHADAP KUALITAS SEMEN BEKU BABI LANDRACE DALAM PENGENCER DURASPERM MODIFIKASI DENGAN AIR BUAH LONTAR (Effect of different equilibration time on the quality of landrace boar frozen semen on modification diluent....

“…(Bebas dan Gorda, 2020) melaporkan volume 180 mL; dan (Banamtuan et al 2021), melaporkan volume 101,75±20,46 mL. Perbedaan rataan volume semen dari berbagai penelitian disebabkan karena jenis ternak, usia, berat badan, frekuensi pengambilan semen, lingkungan dan metode koleksi semen (Sangma et al, 2020).…”

“…Perbedaan rataan volume semen dari berbagai penelitian disebabkan karena jenis ternak, usia, berat badan, frekuensi pengambilan semen, lingkungan dan metode koleksi semen (Sangma et al, 2020). Hasil ini juga tidak berbeda jauh dengan laporan Yusuf et al (2017), yakni semen berwarna putih-krem dengan konsistensi encer dan pH 6,72±0,10; ; menurut Banamtuan et al (2021), semen yang dihasilkan berwarna putihkrem, konsistensi encer dan pH 6,8; dan Knox (2011), melaporkan semen berwarna putih susu dengan konsistensi encer; Gadea (2003) pH 7,4±0,2. Kualitas semen segar yang diperoleh adalah motilitas 70,00%, viabilitas 85,82± 4,45%, abnormalitas 3,46±0,33% dan konsentrasi 270,25±60,83 x 106/mL.…”

“…Nilai membran plasma utuh yang diperoleh adalah 86,65 ± 4,22%, hasil ini tidak berbeda jauh dari penelitian yang dilakukan oleh Sangma et al (2020), yang melaporkan nilai MPU 81,74±0,45% dan sedikit lebih tinggi dari yang dilaporkan oleh Banamtuan et al (2021), yakni 90,79±2,79%. Perbedaan nilai membran plasma utuh dipengaruhi bangsa, pola pemeliharaan dan kandungan pengencer (Lubis et al, 2013).…”