Um cão, fêmea, poodle, 8 anos, apresentou queixa principal de lesão ulcerada em plano nasal e epixtase, com evolução de aproximadamente seis meses. O animal foi submetido a anestesia para retirada do nódulo de 0,5 cm de diâmetro. O material foi encaminhado para exame histopatológico de rotina com coloração de Hematoxilina-Eosina (HE) e respectivamente imuno-histoquímica. No exame histopatológico notou-se uma massa nodular, com bordos mal definidos, constituída por células arredondadas de citoplasma escasso e cromatina nuclear condensadas que iam desde a derme profunda e dissecavam parte da epiderme. Essas células apresentavam um padrão de pleomorfismo alto e alta relação núcleo:citoplasma. Entremeando o estroma observou-se grande quantidade de estruturas vasculares que vão desde a superfície basal até a derme superficial. O exame imuno-histoquímico revelou forte marcação para o complexo principal de histocompatibilidade MHCII, que cora fortemente linfócitos e histiócitos, que correspondem a estrutura tumoral e proliferativa das células neoplásicas do tumor. Os tumores histiocíticos, bem como histiocitomas são relativamente comuns em cães jovens e mais raramente os histiocitomas fibrosos malignos são diagnosticados em cães, ocorrendo mais comumente em humanos. Nesse relato são descritas as características patológicas e imuno-histoquímicas que permitiram o diagnóstico, possibilitando a diferenciação entre histiocitomas não fibróticos e outras neoplasias caninas comuns.

Cases of periodontitis in sheep from the Agreste and Zona da Mata regions of Pernambuco, Northeastern Brazil, are described. Information on breed, age and sex of affected animals as well as clinical and pathological aspects of the disease were obtained during farm visits. Fifteen animals from a total of 450 Santa Inês sheep, aged between three and twelve years, showed low body condition score, dull coat, and difficulty in feeding followed by pain on palpation of the mandibular region. Physical examination of the oral cavity of affected animals and post mortem findings of slaughtered individuals revealed varying degrees of periodontitis, wear, fracture, mobility, and loss of teeth associated with gingival retraction, presence of biofilm and food packing between incisors, premolars and molars teeth. Increased mandibular volume was observed in eight animals and, in two of them, an abscess fistula, exclusively in mandibular teeth. The occurrence of periodontitis in sheep had not been recorded until now in Northeastern Brazil and, thus, this is the first description of cases in the region.

The skin of donkeys and mules represents a promising source of income; however, cultural, productive, and infectious factors can directly interfere with the quality of the integumentary tissue and well-being of these species. The objective of this study is to present a literature review on equine dermatopathies. This literature review included scientific articles related to equine medicine and breeding according to pre-established search terms and expressions published in recently articles. The evaluation of the clinical and pathological behavior of dermatopathies implies the use of control strategies and the recognition of pathological patterns that may be particular to the species.

Trypanosoma vivax is a protozoan originating from the African continent, which, although it has not yet been able to complete its biological cycle in South America, due to the absence of the tsetse fly, can still cause death in ruminants. The objective of this study was to verify the effects of T. vivax on the measurements and indices in sheep that characterize animal performance, as well as on economic losses in meat animals. Twenty intact adult male sheep were used for this study, all of approximately the same ages and weights, reared in confinement, and subjected to the same management and diet, which was balanced and supplemented with adequate minerals. The animals were divided into two groups: the control group (CG) and the infected group (IG), which was inoculated intravenously with 1.3 x 10 5 trypomastigotes of T. vivax. Feed intake was verified daily, whereas the feed conversion (FC), feed efficiency index (FEI), and weight gain were obtained weekly. Total weight gain (TWG) was determined after 70 days post-infection. The economic loss was calculated by subtracting the value obtained (IG) from the expected value (CG), and the difference was expressed as a percentage. A randomized block design was used to isolate the effect of the initial weight. The means were compared by the Student "t" test at 5%. Of the 10 infected animals, one died from the parasitism, yielding a rate much lower than that observed in natural outbreaks. The groups presented similar feed intakes throughout the experimental period; however, the TWG of the infected group was significantly lower (50.7%) than that of the CG. Similarly, the daily weight gain (DWG), feed conversion (FC), and feed efficiency index (FEI) of the IG were significantly lower than those of the CG. In addition, the worst rates of FC and FEI coincided with parasitemia peaks and recurrences, probably due to immunological demand and tissue repair. The abdominal circumference of the infected animals was statistically lower than that of the CG, which may be directly related to fat mobilization, in addition to the possible negative effect on the digestive capacity. The economic losses due to weight alone were 24.07% and could reach 31.66% of the herd value due to animal death. Infection with T. vivax negatively affected animal performance indices, even when the animals were well nourished. Trypanosoma vivax in sheep destined for slaughter caused economic losses of 31.66% due to the decrease in performance and the death rate in the infected group. ResumoO Trypanosoma vivax é um protozoário originário do Continente Africano que mesmo não completando seu ciclo biológico na América do Sul, devido à ausência da mosca tsé-tsé, pode levar ruminantes a óbito. O presente trabalho teve por objetivo verificar, em ovinos infectados, os efeitos do T. vivax em medidas e índices que caracterizam o desempenho animal, bem como perdas econômicas em animais de abate. Para tanto foram utilizados vinte ovinos adultos, machos, inteiros com idade e peso aproximados, criados em confina...

This case study describes the cytological and histopathological findings of cutaneous masses in a bovine, including a peripheral nerve sheath tumor (PNST), vaccine-associated granulomatous inflammation, and eosinophilic inflammation due to parasitosis. A six-year-old undefined cow (SRD) presented with heterogeneous cutaneous lesions including multiple nodules in the left paralumbar fossa, bilaterally at the withers, and scattered along the dorsum, limbs and near the tail; some lesions were associated with ticks. Cytology of these nodules showed benign mesenchymal neoplasia (paralumbar fossa), granulomatous and pyogranulomatous inflammation (withers) and keratin (dorsum). Histopathology, in this order, confirmed PNST, post-vaccination granuloma, and eosinophilic dermatitis. A peripheral nerve sheath tumor was suspected based on the histological findings, showing a well-delineated proliferation of fusiform cells arranged in plexiform structures, which appeared red by Masson's Trichrome stain. The diagnosis was confirmed by immunohistochemistry (anti-S100 antibody). Vaccine reaction often occurs in cattle, and cytological examination is sufficient to determine the inflammatory process. Eosinophilic dermatitis is usually accompanied by perivascular inflammation and reflects the exfoliative process by the oral apparatus of the parasite. ResumoDescrevem-se os achados citológicos e histopatológicos do tumor de bainha de nervo periférico (TBNP), da reação vacinal e da inflamação eosinofílica decorrente dapicada de carrapatoem um bovino. Uma vaca sem raça definida (SRD) de seis anos de idade foi apresentada com diferentes lesões cutâneasnodulares localizadas na fossa paralombar esquerda, bilateralmente na cernelha e dispersos no dorso, membros e próximo à cauda, por vezes associado a carrapatos. Realizou-se citologia e biópsia desses nódulos. Na citologia verificou-se neoplasia mesenquimal benigna (fossa paralombar), inflamação granulomatosa e piogranulomatosa (cernelha) e ceratina (dorso). Na histopatologia, confirmou-se que esses nódulos correspondiam, nessa ordem, a tumor de bainha de nervo periférico, granuloma vacinal e dermatite eosinofílica. O diagnóstico do TBNP foi estabelecido com base nos achados histológicos, que caracterizaram-se por uma proliferação bem delimitada de células fusiformes arranjadas em estruturas plexiformes, corados em vermelho pelo Tricômico de Masson, e confirmado por imuno-histoquímica (anticorpo anti-S100). A reação vacinal ocorre frequentemente em bovinos e o exame citológico é suficiente para determinação do processo inflamatório. Dermatite eosinofílica em geral é acompanhada de inflamação perivascular e perianexal e reflete a ação esfoliativa do aparato bucal do parasita. Palavras-chave: Dermatopatia. Inflamação eosinofílica. Inflamação granulomatosa. Ruminante. Tumor de bainha do nervo periférico.

Newcastle disease virus (NDV), also known as avian paramyxoviruses 1 (APMV-1) is among the most important viruses infecting avian species. Given its widespread circulation, there is a high risk for the reintroduction of virulent strains into the domestic poultry industry, making the surveillance of wild and domestic birds a crucial process to appropriately respond to novel outbreaks. In the present study, we investigated an outbreak characterized by the identification of sick pigeons in a large municipality in Northeastern Brazil in 2018. The affected pigeons presented neurological signs, including motor incoordination, torticollis, and lethargy. Moribund birds were collected, and through a detailed histopathological analysis we identified severe lymphoplasmacytic meningoencephalitis with perivascular cuffs and gliosis in the central nervous system, and lymphoplasmacytic inflammation in the liver, kidney, and intestine. A total of five pigeons tested positive for NDV, as assessed by rRT-PCR targeted to the M gene. Laboratory virus isolation on Vero E6 cells confirmed infection, after the recovery of infectious NVD from brain and kidney tissues. We next characterized the isolated NDV/pigeon/PE-Brazil/MP003/2018 by next-generation sequencing (NGS). Phylogenetic analysis grouped the virus with other NDV class II isolates from subgenotype VI.2.1.2, including two previous NDV isolates from Brazil in 2014 and 2019. The diversity of aminoacid residues at the fusion F protein cleavage site was analyzed identifying the motif RRQKR↓F, typical of virulent strains. Our results all highlight the importance of virus surveillance in wild and domestic birds, especially given the risk of zoonotic NDV.

Cutaneous melanoma is a neoplasm originating from melanocytes. In equines, a variable incidence of 6 to 14% of the total tegumentary neoplasms. The occurrence of metastasis is uncommon. In this sense, the objective was to describe a case of metastatic cutaneous melanoma in the equine species. A 15-year-old mixed-breed mare, died and was sent to perform necroscopic examination. During the processing of samples of various tissues and organs submitted to histopathological analysis. The main macroscopic alterations observed corresponded to proliferations in cutaneous glandular tissue of the udder, mammary lymph nodes, heart, lung, parietal pleura, liver and spleen, with sometimes blackened cutting surface. The morphological pattern, throughout the udder tissue extension, was compatible with melanoma. Histopathological evaluation of the two affected organs revealed a similar microscopic appearance. When associating the anatomopathological findings, a picture of metastatic cutaneous melanoma was defined. Melanoma should be considered as one of the possible diseases that affect the integument wrapping of the udder of mares of advanced age. A thorough evaluation of the animal is useful, since the metastatic potential is a real possibility.

Background: Myxosarcoma is a malignant mesenchymal tumor that arises from fibroblasts and is characterized by a low frequency of metastases, which in turn are highly invasive locally. Even though this type of tumor is relatively well documented, its radiographic and cytomorphological aspects are rarely described. The present report describes a case of cutaneous myxosarcoma in a female dog, and highlights radiographic, cytological, and histopathological findings. Case: An 11-year-old female mongrel dog was examined at the Veterinary Hospital of Federal University of Paraíba. The patient exhibited a firm 20 cm-wide mass covered by skin in the left abdominal wall, a lesion that had been developing for approximately five months. During the clinical examination, the patient demonstrated apathy and difficulty to stand. Initially, exams requested included radiography, ultrasonography, and cytological analysis of biopsy material obtained by fine needle aspiration (FNA). Radiography and ultrasonography were used to investigate the extent of the tumor, as well as invasion of other organs. Radiography revealed that the tumor was radiopaque, homogeneous, and restricted to the skin and subcutaneous tissue; no metastases were observed. Cytological examination allowed observation of a hypocellular sample predominantly composed of isolated pleomorphic fusiform cells embedded in a discrete homogeneous eosinophilic material. These cells exhibited a moderate, well delimited cytoplasm with multiple vacuoles; the end opposed to the nucleus was slender. Their nuclei were eccentric, with punctate chromatin and inconspicuous nucleoli. The patient was subjected to complete surgical excision of the tumor, and the surgical piece was submitted to histopathological examination. The mass was described as single and firm; its dimensions were 20 cm x 16 cm x 15 cm, and its weight was 2.5 kg. Fragments were processed and stained with hematoxylin and eosin (HE) or alcian blue (AB) for evaluation by optical microscopy. Histopathology revealed an infiltrative, expansive, non-encapsulated mass characterized by mesenchymal cells ranging from elongated to stellate. These cells were well individualized, had a scant to moderate cytoplasm, indistinct and slightly eosinophilic borders, and a nucleus that varied from oval to stellate in shape, with highly condensed chromatin and inconspicuous nucleoli. AB-stained specimens exhibited an extensive myxomatous matrix stained in blue, which was intertwined with neoplastic cells. Therefore, the diagnosis of cutaneous myxosarcoma was confirmed. Discussion: The diagnosis of myxosarcoma was established on the basis of radiographic and cytological findings, and it was confirmed by histopathological examination, which sped up decision-making and completion of the case. Tumors of this group are not accompanied by paraneoplastic syndromes; however, given the size and weight of the mass under study, pain may have been the triggering factor of the apathy and difficulty to stand observed here. The age of the patient and t...