Um cão, fêmea, poodle, 8 anos, apresentou queixa principal de lesão ulcerada em plano nasal e epixtase, com evolução de aproximadamente seis meses. O animal foi submetido a anestesia para retirada do nódulo de 0,5 cm de diâmetro. O material foi encaminhado para exame histopatológico de rotina com coloração de Hematoxilina-Eosina (HE) e respectivamente imuno-histoquímica. No exame histopatológico notou-se uma massa nodular, com bordos mal definidos, constituída por células arredondadas de citoplasma escasso e cromatina nuclear condensadas que iam desde a derme profunda e dissecavam parte da epiderme. Essas células apresentavam um padrão de pleomorfismo alto e alta relação núcleo:citoplasma. Entremeando o estroma observou-se grande quantidade de estruturas vasculares que vão desde a superfície basal até a derme superficial. O exame imuno-histoquímico revelou forte marcação para o complexo principal de histocompatibilidade MHCII, que cora fortemente linfócitos e histiócitos, que correspondem a estrutura tumoral e proliferativa das células neoplásicas do tumor. Os tumores histiocíticos, bem como histiocitomas são relativamente comuns em cães jovens e mais raramente os histiocitomas fibrosos malignos são diagnosticados em cães, ocorrendo mais comumente em humanos. Nesse relato são descritas as características patológicas e imuno-histoquímicas que permitiram o diagnóstico, possibilitando a diferenciação entre histiocitomas não fibróticos e outras neoplasias caninas comuns.

Puma yagouaroundi (gato-mourisco) é um felino que apresenta vasta distribuição geográfica no Brasil, podendo ser encontrado em vários biomas. Na literatura observa-se escassez de trabalhos sobre sua anatomia macroscópica. O objetivo do trabalho foi a descrição macroscópica do sistema reprodutor masculino visando a obtenção de informações que possam ser úteis ao manejo reprodutivo da espécie. O estudo foi realizado em um cadáver macho doado ao Laboratório de Anatomia Veterinária pelo Hospital Veterinário da Universidade Federal de Campina Grande. O animal foi fixado em formal a 10 % para posterior dissecação e descrição do sistema reprodutor masculino. Foram identificadas as seguintes estruturas funcionais do aparelho genital masculino do gatomourisco: escroto, pênis, testículos, epidídimos, ductos deferentes e as glândulas genitais acessórias, identificando-se apenas a próstata, não possuindo as glândulas bulbouretrais e vesiculares. O escroto com seus constituintes internos situa-se na região subanal. Os testículos apresentaram formato ovóide, posicionado perinealmente, dentro do escroto. O ducto deferente compreende uma estrutura longa, contínua a cauda do epidídimo, desembocando na superfície craniodorsal da próstata. O pênis mostrou-se um órgão curto, achatado látero-lateralmente com presença de espículas e localizado dentro do prepúcio.Palavras Chaves: descrição macroscópica, gato mourisco, sistema reprodutor Gross anatomy of the reproductive organs of male Puma yagouaroundi (Geoffroy, 1803)ABSTRACT: The Puma yagouaroundi (cat-Moorish) is a feline that has wide geographic distribution in Brazil, can be found in various biomes. In the literature there has been few studies on their gross anatomy. The objective was the macroscopic description of the male reproductive system in order to obtain information that might be useful to reproductive management of the species. The study was conducted in a male body donated to Veterinary Anatomy Laboratory at the Veterinary Hospital of the Federal University of Campina Grande. The animal was fixed in 10% formal for further dissection and description of the male reproductive system. the following functional structures of the male genital tract of the cat-Moorish were identified: scrotum, penis, testicles, epididymis, vas deferens and accessory genital glands, identifying only the prostate, not having the bulbourethral and vesicular glands. The scrotum, with its internal components is located in subanal region. The testes showed ovoid shape, positioned perinealmente within the scrotum. The vas deferens comprises a long structure, continuous tail of the epididymis, ending up in Article History:

The skin of donkeys and mules represents a promising source of income; however, cultural, productive, and infectious factors can directly interfere with the quality of the integumentary tissue and well-being of these species. The objective of this study is to present a literature review on equine dermatopathies. This literature review included scientific articles related to equine medicine and breeding according to pre-established search terms and expressions published in recently articles. The evaluation of the clinical and pathological behavior of dermatopathies implies the use of control strategies and the recognition of pathological patterns that may be particular to the species.

A 4-mo-old northern red-shouldered macaw ( Diopsittaca nobilis) was admitted to the veterinary hospital of the Arruda Câmara Zoo, in the State of Paraiba, Brazil, for investigation of an orbital mass. Given rapid progression and lack of response to treatment, the bird was euthanized, and an autopsy was performed. Histologically, the mass consisted of a retrobulbar invasive tumor characterized by tubular and rosette-like structures, with interspersed heteroplastic tissues, such as aggregates of neuroglial cells and islands of hyaline cartilage. The tumor was immunopositive for pancytokeratin, GFAP, NSE, and S100. These findings were compatible with an ocular teratoid medulloepithelioma, a neoplasm best described in humans but also reported rarely in young cockatiels and African Grey parrots.

This case study describes the cytological and histopathological findings of cutaneous masses in a bovine, including a peripheral nerve sheath tumor (PNST), vaccine-associated granulomatous inflammation, and eosinophilic inflammation due to parasitosis. A six-year-old undefined cow (SRD) presented with heterogeneous cutaneous lesions including multiple nodules in the left paralumbar fossa, bilaterally at the withers, and scattered along the dorsum, limbs and near the tail; some lesions were associated with ticks. Cytology of these nodules showed benign mesenchymal neoplasia (paralumbar fossa), granulomatous and pyogranulomatous inflammation (withers) and keratin (dorsum). Histopathology, in this order, confirmed PNST, post-vaccination granuloma, and eosinophilic dermatitis. A peripheral nerve sheath tumor was suspected based on the histological findings, showing a well-delineated proliferation of fusiform cells arranged in plexiform structures, which appeared red by Masson's Trichrome stain. The diagnosis was confirmed by immunohistochemistry (anti-S100 antibody). Vaccine reaction often occurs in cattle, and cytological examination is sufficient to determine the inflammatory process. Eosinophilic dermatitis is usually accompanied by perivascular inflammation and reflects the exfoliative process by the oral apparatus of the parasite. ResumoDescrevem-se os achados citológicos e histopatológicos do tumor de bainha de nervo periférico (TBNP), da reação vacinal e da inflamação eosinofílica decorrente dapicada de carrapatoem um bovino. Uma vaca sem raça definida (SRD) de seis anos de idade foi apresentada com diferentes lesões cutâneasnodulares localizadas na fossa paralombar esquerda, bilateralmente na cernelha e dispersos no dorso, membros e próximo à cauda, por vezes associado a carrapatos. Realizou-se citologia e biópsia desses nódulos. Na citologia verificou-se neoplasia mesenquimal benigna (fossa paralombar), inflamação granulomatosa e piogranulomatosa (cernelha) e ceratina (dorso). Na histopatologia, confirmou-se que esses nódulos correspondiam, nessa ordem, a tumor de bainha de nervo periférico, granuloma vacinal e dermatite eosinofílica. O diagnóstico do TBNP foi estabelecido com base nos achados histológicos, que caracterizaram-se por uma proliferação bem delimitada de células fusiformes arranjadas em estruturas plexiformes, corados em vermelho pelo Tricômico de Masson, e confirmado por imuno-histoquímica (anticorpo anti-S100). A reação vacinal ocorre frequentemente em bovinos e o exame citológico é suficiente para determinação do processo inflamatório. Dermatite eosinofílica em geral é acompanhada de inflamação perivascular e perianexal e reflete a ação esfoliativa do aparato bucal do parasita. Palavras-chave: Dermatopatia. Inflamação eosinofílica. Inflamação granulomatosa. Ruminante. Tumor de bainha do nervo periférico.

Goats and sheep have morphological characteristics for adaptation to desert and semiarid regions. The appearance of scrotum division known as scrotum bipartition has already been reported in goats. This anatomy increases the surface of each testicle exposed to environmental temperature, favoring heat dissipation and improving reproductive efficiency. Considering that there are already studies on the goat species demonstrating the presence of this characteristic as an influence on reproductive parameters, the prevalence of scrotum bipartition was estimated in the sheep herds reared in the municipality of Patos, Paraiba backwoods, Brazil. A total of 331 rams were examined from farms in four municipalities in the micro-region of Patos, Paraiba, Brazil, and the same study was also carried out at the municipal slaughterhouse in this city, where 456 animals were examined. According to the analysis, 66.67% of the farms visited presented one or more sheep with scrotum bipartition, with a prevalence of 11.48% on the farms and 14.47% at the slaughterhouse. The degree of bipartition was 9.59 ± 1.035% of the total scrotum length for the animals in the field and 12.89 ± 0.749% for those from the slaughterhouse, characterizing bipartition of less than 50% of the scrotum length. The variables intensive rearing (OR = 16.6) and the Dorper breed (OR = 6.91) were identified as factors associated to the presence of scrotum bipartition. It was concluded that scrotum bipartition is a characteristic present in sheep reared in the municipality of Patos in the semiarid region of Paraiba state, northeastern Brazil, and high prevalence was observed of farms with bipartition sheep, but a low number of animals with scrotum bipartition was identified.

Background: Histiocytic tumors in felines are nodules that commonly develop on limbs and head extremities. They can be divided into many subtypes including cutaneous histiocytoma, histiocytic sarcoma, reactive fibrohistiocytic nodule, Langerhans cell histiocytosis, and progressive feline dendritic cell. Despite the same origin, they have behaviors that differ from each other, thus it is important to confirm diagnosis with histopathological and immunohistochemical tests, because early identification can facilitate prognosis and treatment. In this study, we describe the pathological and immunohistochemical characteristics, enabling differentiation feline neoplasms derived from histiocytes. Case: A 5-year-old, crossbreed, male, feline presented with a nodulation at the base of the left ear. The mass was slow growing, partially alopecic, with no other changes associated with tumor development. The nodule was round and circumscribed, movable, with an elevated surface. He was referred for surgery and an elliptical sample around the tumor was carefully dissected. Routine histopathological evaluation was performed with hematoxylin and eosin (HE), as well as immunohistochemistry. Histopathology showed circumscribed proliferation of histiocytic cells, with abundant and eosinophilic cytoplasm. The proliferative cells were large and rounded, extending from the superficial dermis and basement membrane to the deep dermis. At the extremities, some cells had visible vacuoles. Mitotic activity ranged from 3 to 4 mitoses per field in 40x magnification. Immunohistochemistry showed positive staining for histocompatibility complex MCII and lysozyme antibodies, marking histiocytic cells. Labeling was positive for CD20 in cells of lymphoid lineage B and negative for E-cadherin. Histiocytic cells did not invade the epidermis; hence, proliferation was classified as nonepitheliotropic. These methods contribute to the literature regarding the diagnosis of this rare tumor. Therefore, histological as well as immunohistochemical evaluation are important bfor confirming clinical diagnosis of histiocytic proliferation non-epitheliotropic. Discussion: Progressive histiocytosis of feline dendritic cells, in both epitheliotropic and non-epitheliotropic forms, is considered a clinically progressive and rare disorder. There are reports which include cytological, clinical, histological and immunohistochemical examinations, but the diagnostic characteristics regarding the non-epitheliotropic classification have not yet been properly identified. Nodulations are predominantly observed in head and limb regions, usually non-ulcerated, which can both increase and decrease in size, and are typically painless. The tumor in the present case was restricted to the base of the ear and no evidence of infiltration or metastasis was found. Progressive histiocytosis may spread and reach the lymphatic system through the lymph nodes, subsequently becoming systemic. The non-aggressive behavior observed in this case is possibly related to the non-epitheliotropic pattern. In the present case, MHC II histocompatibility complex markers, a phenotype compatible with dendritic cells, were used. Lysozyme antibodies marked histiocytic cells and the reactive lymphoid infiltrate was composed of CD20-positive B lymphoid lineage cells. Staining for E-cadherin was negative, negative results in labeling experiments is common, it is dependent upon the cellular origin of the leukocytes present in the sample. Staining for these molecules is recommended for differentiating feline progressive histiocytosis from Langerhans cells. Langerhans cells can be characterized by E-cadherin expression in about 10% of cases and marked T lymphocyte and neutrophil expression in the affected tissue. In this case, the histopathological exam along with immunohistochemistry was essential for differentiating them.

Descreve-se os achados clínicos e patológicos do carcinoma pancreático em dois cães. O primeiro caso se refere a um cão sem raça definida, de quatro anos de idade, com histórico de anorexia, prostração e dispneia, vindo a óbito durante atendimento clínico. O segundo caso se refere a um cão sem raça definida, de oito anos de idade, com histórico de anorexia, adipsia e êmese há dez dias, sem melhora clínica, sendo eutanasiado posteriormente. Em ambos os casos os animais foram encaminhados para necropsia e amostras de tecidos foram enviadas para histopatologia e imuno-histoquímica. No caso 1, os principais achados macroscópicos consistiram em icterícia difusa e presença de massa expansiva, brancacenta, macia e gelatinosa no pâncreas e com metástase para estômago, fígado, rins, músculo abdominal, intestino e pele. Enquanto no caso 2, essa massa envolvia apenas pâncreas, omento e fígado. Em ambos os casos a histopatologia revelou massa não encapsulada, expansiva, constituída por proliferação epitelial pleomórfica arranjada em túbulos, cujo interior possuia material basofílico mucinoso. A mesma proliferação foi observada em estômago, fígado, rins, músculo abdominal, intestino e pele (caso 1) e em omento e fígado (caso 2). O diagnóstico de carcinoma pancreático foi estabelecido através dos achados histopatológicos, histoquímicos e imuno-histoquímico. Dentre os carcinomas pancreáticos destacam-se os neoplasmas mucinosos intraductais papilares, frequentemente diagnosticado em humanos e menos comumente em cães. Esta condição foi observada em cães adultos sem raça definida no Agreste da Paraíba e deve ser inserida no diagnóstico diferencial de patologias do trato gastrointestinal e hepáticas de cães.