Induction of resistance in common bean (Phaseolus vulgaris) has been considered a promising alternative to control anthracnose. Among the changes generated in the induction of resistance, structural changes have been reported by several authors as an efficient form of resistance to the stress plants undergo. Histochemical analysis techniques have been used to investigate tissue changes triggered by induction of resistance. Thus, this study aims to investigate certain histochemical changes suffered by common bean plants induced with potassium phosphite and Trichoderma spp. in response to the attack by Colletotrichum lindemuthianum, aiming to determine the host response pattern in terms of structural changes, associating it to possible disease control. Treatments consisted of isolates T. harzianum (isolate TOD1) and T. virens (isolate TM4), leaf fertilizer potassium phosphite Fertilis®, and distilled water (control). Inducers were applied to common bean alone or associated, consisting of five treatments plus the control treatment. The six treatments were evaluated for the absence and presence of C. lindemuthianum in a factorial scheme (6×2). Treatments allowed evaluating the severity of anthracnose in common bean, location of H2O2, lignin deposition, and hypersensitivity response in common bean hypocotyl by histochemical staining. Potassium phosphite and combinations of T. virens and T. harzianum with potassium phosphite efficiently reduced disease severity under greenhouse conditions, reaching 68, 84, and 71%, respectively. Studies with hypocotyl showed that T. harzianum + potassium phosphite and T. virens + potassium phosphite accelerated the H2O2 accumulation process and lignin deposition at the pathogen penetration site, in addition to the hypersensitivity reaction through the resistance-inducing activity, contributing to the protection of common bean against anthracnose caused by C. lindemuthianum.
Basidiomycete fungi that decompose wood produce substances with promising biological activity for the alternative control of plant diseases. The production of these substances can change according to the climatic conditions and the substrate used for fungal cultivation. The objective of this study was to develop a substrate with sawdust from Eucalyptus sp. and to verify its influence on biomass and cinnabarin production by Pycnoporus sanguineus. Sawdust was used in two particle sizes: less than 500 microns (G1) and between 500–841 microns (G2). Four isolates of P. sanguineus were plated on Petri dishes containing potato broth and agar media added with 0%, 1%, 5%, 10%, and 15% sawdust for each particle size. The largest final diameter of the colony and speed of mycelial growth were observed in the substrate with G1 particle size, with the Ps14 isolate showing the highest averages. For these variables, the sawdust concentration did not influence G1 granulometry and provided the highest values in G2 granulometry. Fresh mycelium mass and cinnabarin production showed the highest values in G2, with the isolated Ps13 and Ps08 showing the highest averages, whereas in G1, Ps14 had the best performance for the analyzed variables. These results indicate that sawdust from Eucalyptus sp., at concentrations of 10% and 15%, is an alternative for the in vitro cultivation of P. sanguineus, and that particle size influences the growth speed, fresh mass production, and cinnabarin content.
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