The study was conducted to examine the histological changes i.e. morphology and biometry of immune organs (thymus, spleen and bursa cloacalis or «Fabricius») of broilers in response to dietary dexamethasone (DEX). The day old chicks were obtained from the commercial hatchery and randomly divided into two groups i.e. control and experimental or treated group. The control group was reared on commercial broiler ration and the experimental group (n=25) was maintained on commercial broiler ration with corticosteroid (Dexamethasone-Decason, BP 0.5 mg, Opsonin @ 7 mg/kg feed). Samples (bursa cloacalis, spleen, and thymus) were collected from the ten control and ten experimental broilers at 14 and 28 days of experiment; then tissues were stained with Hematoxylin and Eosin. The biometric measurements of the samples were performed by the calibrated stage micrometer. Finally, the obtained data were analyzed using GraphPad Prism 8 software. In DEX treated group, the morphology of thymus, spleen and bursa cloacalis did not show any abnormal alterations. But their development rate was slower on visual inspection in DEX treated group. The length and width of bursal follicle of bursa cloacalis, thymic lobule of thymus and white pulp of spleen were statistically consisted but numerically decreased in DEX treated group than the control. The present findings suggested that DEX does not affect the histological architectures of immune organs except causing developmental arrest. Numerical decrease in the biometry of immune organs indicates that DEX causes apoptosis of immune cells in lymphoid organs of broiler.
The radiofrequency electromagnetic radiation emitted by smart phones on biological systems has wide media coverage and public concern in recent years. The aim of this study was to explore the effects of fourth-generation cell phone radiation exposure on hematological (Total leukocyte count, Total erythrocyte count, and hemoglobin %), biochemical (Serum creatinine) parameters, and histopathological changes in the kidney and testis of Swiss albino mice. A total of 30 male Swiss albino mice weighing 45–65 g was randomly divided into three groups ( n = 10). The first group A was the control group, the second group B, was exposed to 40 minutes of mobile phone radiation daily, the third group C was exposed to 60 minutes of radiation daily from two 2400 Megahertz fourth-generation connected mobile phones for 60 days, respectively. The electromagnetic radiation frequency radiometer measured the frequency of electromagnetic radiation emitted from cell phones. The specific absorption rate was calculated as 0.087 W/kg. The control group was kept under similar conditions, but the electromagnetic field was not given for the same period. All the mice were sacrificed at the end of the experiment. The blood samples were collected for hematobiochemical study, and then kidney and testis tissues were collected for histopathological study. Results of the study showed that the body weight and total erythrocyte count values were significantly (p < 0.05) decreased while total leukocyte count, hemoglobin %, and serum creatinine values were significantly (p < 0.05) increased in both the radiation exposure groups relative to the control group. Histopathological observation showed the kidney of 60 minutes exposed mice interstitial inflammation that causes marked mononuclear cellular infiltration compared to the 40 minutes and control mice. Compared to control mice, histopathological examinations of testicular tissue from the exposed mice, showed irregular in shapes and non-uniform sizes and fewer spermatogenic cells layer that leads to the larger lumen in the seminiferous tubules. It is concluded that fourth-generation cell phone radiation exposure may affect blood hemostasis and inflammation of mice's kidney and testis tissue. Based on these studies, it is important to increase public consciousness of potential adverse effects of mobile phone radiofrequency electromagnetic radiation exposure.
Formaldehyde (FA) is toxic over a range of doses and of particular concern to anatomists and medical students on laboratory use. FA induces several characteristics of neurotoxicity in addition to systemic effects. Therefore, to know the toxic effects of FA on brain and lungs, mice were collected from international center for diarrheal disease research. They were divided into four groups i.e. control, inhalation, oral and intraperitoneal. The inhalation, oral and intraperitoneal exposure groups further divided into three subgroups which were subjected to exposure of FA daily for 30 days in case of inhalation and oral groups and 10 days in intraperitoneal group for acute toxic effects. Morphological study showed gross abnormalities i.e. congestion on lungs. But no gross abnormal features observed on brain. In 5 ppm treated inhaled group, lungs tissue revealed hemorrhages. In the inhaled groups, brain tissue showed degenerating neurons with either pyknotic or karyorrhectic nuclei are gradually increased in highest concentration group. These data supports the view that FA has adverse effects on the morphology of brain and lungs.
Formaldehyde (FA) is a very reactive one-carbon compound, can react with lipids, proteins, and nucleic acids which are cellular components.FA induces cellular toxic effects in the liver by damaging hepatic parenchyma and impairment of functions. This study was carried out to evaluatethe serum biochemical changes and cytotoxic effects on liver in Swiss albino mice caused by FA toxicity. For this purpose, mice were divided into threeequal groups i.e. Control, oral andintraperitoneal.Oral and intraperitonealgroups were further divided into three subgroups which were subjected to exposure of FA for 30 and 10 consecutive days respectively. After exposure, blood and liver samples were collected and analyzed for biochemical and morphological studies. The serum biochemical parameters like Aspartate Transaminase (AST) and Alanine Transaminase (ALT) were increased significantly (P‹0.05) in mice after FA exposure. The anatomical results revealed gross morphological changes i.e. congestion and petechial hemorrhages on the liver. Histologically, the liver showedscattered lymphocytic infiltration, dilatation of sinusoids, necrosis and degeneration of parenchymatous cells in orally exposed mice (10 mg/kg) and diffuse lymphocytic infiltration, necrosis were seen in intraperitoneallyFA injectedmice at the rate of 7 and 10 mg/kg body weight.All these findings revealed that, FA depending on specific dose leads to an irritant toxic effects on the liver of mice.
Background: Because of the unfavorable side effects and higher cost of synthetic anti-diabetic medicines, an alternative approach to manage diabetes mellitus from an herbal source would be safe and within the affordability of the common people. Hence we investigated the hypoglycemic and pancreatic islets restoration effect of Syzygium cumini seed extract using glibenclamide as positive control. Methods: Six (06) normal control mice and eighteen (18) alloxan induced diabetic mice grouped as diabetic control, Syzygium cumini and standard drug, were used for this experiment. In comparison to a reference drug-glibenclamide (@600μg/kg body weight), Syzygium cumini seed ethanolic extract (@500mg/kg body weight) was given orally once daily for thirty days to counteract alloxan induced alteration in blood glucose level and pancreatic cells morphology. Fasting blood glucose (FBG) level was evaluated at 15 days intervals, whereas mice were ethically sacrificed to collect pancreas at the completion of the experiment and processed for histological examination. Results: Research results revealed that in both the Syzygium cumini and standard drug treated groups, FBG level were significantly lower than the diabetic control group (P < 0.05). Histologically, smaller islets and necrosis, present in the pancreas of diabetic mice were returned to normal following treatment with Syzygium cumini seed extract and standard anti-diabetic drug. Conclusion: Syzygium cumini seed extract could be recommended as anti-diabetic for humans and animals especially in developing and under developed countries where this plant is readily available and affordable.
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